Distinct Variations in Gene Expression and Cell Composition across Lichen Planus Subtypes
Abstract
:1. Introduction
2. Results
2.1. Clinically Known LP Subtypes Show Distinct Gene Expression Patterns
2.2. LP Subtypes Exhibit Pathway-Specific Differences in Gene Expression
2.3. Variations in Cellular Compositions Distinguish Clinical LP Subtypes
3. Discussion
4. Materials and Methods
4.1. Human Skin Samples
4.2. NanoString Analyses
4.3. Multiplex Immunohistochemistry
4.4. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cell Types | Classical LP | Genital LP | Oral LP | Lichen Planopilaris | NDC |
---|---|---|---|---|---|
Total T cells | 48.8% | 24.7% | 53.0% | 7.04% | 3.61% |
CD4 + T cells | 8.5% | 3.14% | 0.108% | 0.069% | 0% |
CD8 + T cells | 6.44% | 4.87% | 5.27% | 2.17% | 0.373% |
Tregs | 30.3% | 14.6% | 39.2% | 3.51% | 2.29% |
Granzyme B+ cells | 1.15% | 0.619% | 0.434% | 0.110% | 0.117% |
IL17A+ cells | 2.39% | 1.54% | 8.02% | 1.18% | 0.831% |
Keratinocytes | 24.9% | 25.4% | 24.6% | 54.0% | 62.1% |
Total macrophages | 12.3% | 9.26% | 11.2% | 3.96% | 0.193% |
M1 macrophages | 0.0397% | 0.243% | 0.266% | 0.0092% | 0% |
M2 macrophages | 0.176% | 0.191% | 0.124% | 0.212% | 0.0126% |
Neutrophilic granulocytes | 0.301% | 2.51% | 0.730% | 0.446% | 0.0084% |
Other | 13.8% | 38.1% | 10.5% | 34.6% | 34.1% |
Characteristics of Patients | Number of Patients, No. | Age y, Mean (SD) | Female/Male Sex, Ratio | Other Autoimmune Disease, No. | HBV or HCV Infection, No. | Topical Therapy at Time of Biopsy *, No. | Disease Onset Until Biopsy Months, Mean (SD) ** | |
---|---|---|---|---|---|---|---|---|
Classical LP | NanoString | 5 | 64.0 (13.2) | 2/3 | 0 | 0 | 0 | 4 (1.8) |
IHC | 6 | 65.0 (15.3) | 3/3 | 0 | 0 | 0 | 10 (14.8) | |
Oral LP | NanoString | 3 | 49.0 (8.9) | 1/2 | 0 | 0 | 0 | 2 (0.6) |
IHC | 3 | 52.0 (6.6) | 1/2 | 0 | 0 | 0 | 11 (5.7) | |
Genital LP | NanoString | 4 | 59.8 (17.4) | 0/4 | 0 | 1 | 1 | 14 (14.1) |
IHC | 4 | 56.5 (19.6) | 3/1 | 0 | 0 | 0 | 11 (2.7) | |
Lichen planopilaris | NanoString | 5 | 56.2 (17.6) | 5/0 | 2 | 0 | 2 | 24 (14.4) |
IHC | 3 | 45.7 (17.5) | 2/1 | 1 | 0 | 0 | 24 (20.2) | |
Healthy skin (NDC) | NanoString | 3 | 81.0 (7.9) | 1/2 | 0 | 0 | 0 | 0 |
IHC | 4 | 57.0 (20.4) | 0/4 | 0 | 0 | 0 | 0 |
Opal | T-Cell Panel | Macrophage Panel | ||
---|---|---|---|---|
Antibody | Dilution | Antibody | Dilution | |
480 | CD8 | 1:200 | pSTAT1 | 1:50 |
520 | FoxP3 | 1:100 | ||
570 | IL17A | 1:200 | MPO | 1:150 |
620 | GranzymeB | 1:100 | c-Maf | 1:50 |
690 | PanCK | 1:100 | PanCK | 1:100 |
780 | CD4 | 1:100 | CD68 | 1:100 |
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Knoch, C.; Baghin, V.; Turko, P.; Winkelbeiner, N.; Staeger, R.; Wei, K.; Banzola, I.; Mellett, M.; Levesque, M.P.; Kuendig, T.; et al. Distinct Variations in Gene Expression and Cell Composition across Lichen Planus Subtypes. Int. J. Mol. Sci. 2024, 25, 9720. https://doi.org/10.3390/ijms25179720
Knoch C, Baghin V, Turko P, Winkelbeiner N, Staeger R, Wei K, Banzola I, Mellett M, Levesque MP, Kuendig T, et al. Distinct Variations in Gene Expression and Cell Composition across Lichen Planus Subtypes. International Journal of Molecular Sciences. 2024; 25(17):9720. https://doi.org/10.3390/ijms25179720
Chicago/Turabian StyleKnoch, Cadri, Veronika Baghin, Patrick Turko, Nicola Winkelbeiner, Ramon Staeger, Kongchang Wei, Irina Banzola, Mark Mellett, Mitchell P. Levesque, Thomas Kuendig, and et al. 2024. "Distinct Variations in Gene Expression and Cell Composition across Lichen Planus Subtypes" International Journal of Molecular Sciences 25, no. 17: 9720. https://doi.org/10.3390/ijms25179720