Influence of the Chemical Properties of Cereal Grains on the Structure and Metabolism of the Bacteriome of Rhyzopertha dominica (F.) and Its Development: A Cause–Effect Analysis
Abstract
:1. Introduction
2. Results
2.1. Relationship between the Development of R. dominica and Chemical Parameters of Cereal Grains
2.2. Analysis of the Bacteriobiome of R. dominica
2.3. Metabolism of the R. dominica Bacteriobiome
2.4. Global Analysis of the Relationships between the Tested Parameters
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Experiment with Different Feed Variants
4.3. Chemical Analyses of Grains
4.4. Biochemical Properties of Insects
4.5. Statistical Calculations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Generation | Generation I | Generation II | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Species of the | Background without Feed | Wheat | Barley | Wheat | Barley | ||||||||
Cultivar | Impresja | Rusałka | Varius | Trofeum | Radek | Ismena | Impresja | Rusałka | Varius | Trofeum | Radek | Ismena | |
g__Pantoea | 23.3 | 18.9 | 18.6 | 24.2 | 29.2 | 12.9 | 12.3 | 19.7 | 35.3 | 36.0 | 28.0 | 10.8 | 10.1 |
g__Ralstonia | 21.6 | 14.3 | 11.4 | 27.7 | 8.9 | 6.9 | 22.8 | 19.9 | 3.3 | 11.0 | 19.2 | 5.1 | 15.6 |
g__Staphylococcus | 6.0 | 20.3 | 28.7 | 0.0 | 14.5 | 54.9 | 0.7 | 0.0 | 0.0 | 0.0 | 0.0 | 7.8 | 0.4 |
g__Pseudomonas | 9.3 | 17.1 | 9.3 | 11.0 | 10.1 | 6.4 | 11.2 | 6.3 | 5.4 | 8.8 | 10.8 | 5.5 | 22.6 |
g__Stenotrophomonas | 10.8 | 6.4 | 7.9 | 11.4 | 0.9 | 5.7 | 25.3 | 22.3 | 4.7 | 6.1 | 1.2 | 1.8 | 5.4 |
g__Massilia | 7.6 | 5.3 | 4.8 | 10.3 | 8.5 | 2.2 | 7.4 | 9.5 | 5.2 | 12.4 | 9.9 | 1.6 | 8.1 |
g__Acinetobacter | 0.0 | 0.3 | 0.5 | 0.6 | 12.8 | 0.8 | 1.4 | 1.4 | 21.1 | 7.4 | 7.1 | 4.0 | 13.8 |
g__Serratia | 0.0 | 0.4 | 0.7 | 1.7 | 6.4 | 0.6 | 0.0 | 10.3 | 17.7 | 9.1 | 14.1 | 0.7 | 11.1 |
g__Xanthomonas | 0.0 | 3.2 | 8.8 | 6.5 | 0.7 | 4.9 | 7.2 | 2.3 | 0.0 | 2.3 | 1.8 | 14.3 | 0.0 |
g__Sphingomonas | 6.6 | 4.1 | 2.5 | 3.0 | 1.2 | 1.1 | 2.2 | 3.2 | 2.2 | 2.0 | 3.1 | 1.2 | 1.2 |
g__Candidatus_Sulcia | 0.0 | 0.0 | 0.0 | 0.0 | 0.5 | 0.0 | 0.6 | 0.0 | 0.0 | 0.0 | 0.0 | 19.6 | 6.4 |
g__Sphingobium | 4.4 | 2.5 | 0.6 | 0.6 | 0.4 | 1.3 | 1.0 | 1.4 | 1.1 | 1.2 | 0.7 | 0.4 | 0.9 |
o__Enterobacterales | 7.9 | 2.2 | 4.5 | 0.3 | 0.0 | 0.6 | 3.6 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
g__Caulobacter | 0.0 | 1.8 | 0.3 | 1.4 | 0.5 | 0.7 | 0.7 | 0.5 | 0.7 | 1.3 | 0.9 | 0.0 | 0.4 |
g__Paenibacillus | 0.0 | 0.5 | 0.3 | 0.8 | 0.7 | 0.0 | 1.1 | 0.8 | 1.0 | 1.0 | 1.1 | 0.0 | 0.7 |
g__Brevibacterium | 0.0 | 0.0 | 0.0 | 0.0 | 3.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
g__Bosea | 0.0 | 0.7 | 0.0 | 0.0 | 0.2 | 0.0 | 0.2 | 0.3 | 0.3 | 0.4 | 0.3 | 0.4 | 0.6 |
g__Rhizobium | 0.0 | 0.3 | 0.0 | 0.0 | 0.0 | 0.7 | 0.6 | 0.0 | 0.5 | 0.3 | 0.5 | 0.0 | 0.3 |
g__Variovorax | 0.0 | 0.0 | 0.0 | 0.0 | 0.2 | 0.0 | 0.0 | 0.0 | 0.7 | 0.2 | 0.8 | 0.0 | 0.9 |
g__Burkholderia | 2.7 | 0.0 | 0.0 | 0.0 | 0.3 | 0.0 | 0.0 | 1.0 | 0.2 | 0.5 | 0.0 | 0.0 | 0.4 |
g__Bacillus | 0.0 | 0.8 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 4.2 | 0.9 |
g__Cupriavidus | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 1.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
g__Methylobacterium | 0.0 | 0.4 | 0.0 | 0.3 | 0.2 | 0.0 | 0.0 | 1.2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
g__Afipia | 0.0 | 0.6 | 0.2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
g__Escherichia-Shigella | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 2.4 | 0.0 |
g__Corynebacterium | 0.0 | 0.0 | 0.4 | 0.2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.2 |
g__Brevundimonas | 0.0 | 0.0 | 0.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.5 | 0.0 | 0.0 |
g__Alcaligenes | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 5.1 | 0.0 |
g__Sporosarcina | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 1.7 | 0.0 |
f__Comamonadaceae | 0.0 | 0.0 | 0.0 | 0.0 | 0.2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.3 | 0.0 | 0.0 | 0.0 |
g__Conchiformibius | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 1.4 | 0.0 |
g__Ancylobacter | 0.0 | 0.0 | 0.0 | 0.0 | 0.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
g__Aureimonas | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 1.3 | 0.0 |
g__Lysobacter | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
f__Xanthobacteraceae | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.5 | 0.0 | 0.0 | 0.0 | 0.0 |
g__Microvirga | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 4.7 | 0.0 |
g__Pseudarthrobacter | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 4.7 | 0.0 |
g__Candidatus_Berkiella | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.8 | 0.0 |
g__Moraxella | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.7 | 0.0 |
g__Kocuria | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Eudominant | Dominant | Subdominant | Occasional | Casual | |||||||||
>10.01% | 5.1–10.0% | 2.1–5.0% | 1.1–2.0% | <1.0% | |||||||||
Dominance_D | 0.1832 ab | 0.2185 ab | 0.2104 ab | 0.2398 a | 0.6804 a | 0.2033 ab | 0.1595 ab | 0.2579 a | 0.1986 ab | 0.2256 ab | 0.2341 ab | 0.1322 b | |
Shannon_H | 2.0590 ab | 1.7480 b | 1.8110 b | 1.8460 b | 0.7871 b | 1.8510 b | 2.0770 ab | 1.7390 b | 2.0090 ab | 1.7700 ab | 1.9490 ab | 2.2490 a | |
Evenness_e^H/S | 0.5848 ab | 0.5223 ab | 0.5982 ab | 0.5278 b | 0.2632 b | 0.5292 ab | 0.6445 a | 0.4831 b | 0.4973 ab | 0.5703 ab | 0.5866 ab | 0.4989 ab |
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Kosewska, O.; Przemieniecki, S.W.; Nietupski, M. Influence of the Chemical Properties of Cereal Grains on the Structure and Metabolism of the Bacteriome of Rhyzopertha dominica (F.) and Its Development: A Cause–Effect Analysis. Int. J. Mol. Sci. 2024, 25, 10130. https://doi.org/10.3390/ijms251810130
Kosewska O, Przemieniecki SW, Nietupski M. Influence of the Chemical Properties of Cereal Grains on the Structure and Metabolism of the Bacteriome of Rhyzopertha dominica (F.) and Its Development: A Cause–Effect Analysis. International Journal of Molecular Sciences. 2024; 25(18):10130. https://doi.org/10.3390/ijms251810130
Chicago/Turabian StyleKosewska, Olga, Sebastian Wojciech Przemieniecki, and Mariusz Nietupski. 2024. "Influence of the Chemical Properties of Cereal Grains on the Structure and Metabolism of the Bacteriome of Rhyzopertha dominica (F.) and Its Development: A Cause–Effect Analysis" International Journal of Molecular Sciences 25, no. 18: 10130. https://doi.org/10.3390/ijms251810130