Enhancing Lung Recellularization with Mesenchymal Stem Cells via Photobiomodulation Therapy: Insights into Cytokine Modulation and Sterilization
Abstract
:1. Introduction
2. Results
2.1. Decellularization Assessment
2.2. Lung Scaffold Recellularization
3. Discussion
4. Materials and Methods
4.1. Lung Decellularization
4.2. Histological and Immunohistochemistry Analysis of Samples
4.3. Scanning Electron Microscopy (SEM)
4.4. Quantification of DNA in Decellularized Lungs
4.5. Isolation and Culture of Dental Pulp Stem Cells (DPSCs)
4.6. Characterization of Dental Pulp Stem Cells (DPSCs)
4.7. Cultivation of DPSCs in Decellularized Lungs
4.8. Evaluation of Cytokine Levels by ELISA
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Guimarães, L.L.; Brito, A.A.; Cereta, A.D.; Oliveira, A.P.L.; Afonso, J.P.R.; Mello, D.A.C.P.G.; Oliveira-Silva, I.; Silva, C.H.M.; Oliveira, R.F.; Oliveira, D.A.A.P.; et al. Enhancing Lung Recellularization with Mesenchymal Stem Cells via Photobiomodulation Therapy: Insights into Cytokine Modulation and Sterilization. Int. J. Mol. Sci. 2024, 25, 10131. https://doi.org/10.3390/ijms251810131
Guimarães LL, Brito AA, Cereta AD, Oliveira APL, Afonso JPR, Mello DACPG, Oliveira-Silva I, Silva CHM, Oliveira RF, Oliveira DAAP, et al. Enhancing Lung Recellularization with Mesenchymal Stem Cells via Photobiomodulation Therapy: Insights into Cytokine Modulation and Sterilization. International Journal of Molecular Sciences. 2024; 25(18):10131. https://doi.org/10.3390/ijms251810131
Chicago/Turabian StyleGuimarães, Leticia L., Auriléia A. Brito, Andressa D. Cereta, Ana Paula L. Oliveira, João Pedro R. Afonso, Diego A. C. P. G. Mello, Iransé Oliveira-Silva, Carlos H. M. Silva, Rodrigo F. Oliveira, Deise A. A. P. Oliveira, and et al. 2024. "Enhancing Lung Recellularization with Mesenchymal Stem Cells via Photobiomodulation Therapy: Insights into Cytokine Modulation and Sterilization" International Journal of Molecular Sciences 25, no. 18: 10131. https://doi.org/10.3390/ijms251810131