OsMBF1a Facilitates Seed Germination by Regulating Biosynthesis of Gibberellic Acid and Abscisic Acid in Rice
Abstract
:1. Introduction
2. Results
2.1. OsMBF1a Is Ubiquitously Expressed in Rice and Is Induced during Seed Germination
2.2. OsMBF1a Positively Regulates Seed Germination in Rice
2.3. OsMBF1a Modulates Terpenoid-Related Metabolic Pathways
2.4. OsMBF1a Regulated Endogenous ABA and GA Levels in Rice Seed
2.5. Overexpressing ZmMBF1a and ZmMBF1b in Rice Enhances Seed Germination
2.6. ZmMBF1a and ZmMBF1b Overexpression Modulates GA and ABA in Rice
3. Discussion
4. Materials and Methods
4.1. Construction and Planting Materials
4.2. Phenotypic Analysis of Seed Germination
4.3. RNA Extraction and RT-qPCR
4.4. RNA-Seq Analyses
4.5. DAP-qPCR Analyses
4.6. Detection of GA3 and ABA Content
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, X.; Chen, Z.; Guo, J.; Han, X.; Ji, X.; Ke, M.; Yu, F.; Yang, P. OsMBF1a Facilitates Seed Germination by Regulating Biosynthesis of Gibberellic Acid and Abscisic Acid in Rice. Int. J. Mol. Sci. 2024, 25, 9762. https://doi.org/10.3390/ijms25189762
Wang X, Chen Z, Guo J, Han X, Ji X, Ke M, Yu F, Yang P. OsMBF1a Facilitates Seed Germination by Regulating Biosynthesis of Gibberellic Acid and Abscisic Acid in Rice. International Journal of Molecular Sciences. 2024; 25(18):9762. https://doi.org/10.3390/ijms25189762
Chicago/Turabian StyleWang, Xin, Ziyun Chen, Jinghua Guo, Xiao Han, Xujian Ji, Meicheng Ke, Feng Yu, and Pingfang Yang. 2024. "OsMBF1a Facilitates Seed Germination by Regulating Biosynthesis of Gibberellic Acid and Abscisic Acid in Rice" International Journal of Molecular Sciences 25, no. 18: 9762. https://doi.org/10.3390/ijms25189762