The Vital Role of the CAMTA Gene Family in Phoebe bournei in Response to Drought, Heat, and Light Stress
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of PbCAMTA Genes
2.2. Display of the Motif, Domain, and Gene Structure of PbCAMTA Gene Family
2.3. Phylogenetic Analysis of PbCAMTAs
2.4. Chromosome Distribution of PbCAMTAs and Genomic Amplification in P. bournei
2.5. Syntenic Analysis of PbCAMTAs Genes
2.6. Cis-Regulatory Element Prediction of Promoters in 17 PbCAMTA Genes
2.7. Gene Ontology (GO) and KEGG Analysis in PbCAMTA
2.8. Protein–Protein Interaction Network of PbCAMTA Proteins
2.9. Expression Patterns of PbCAMTA Genes in Response to Heat, Drought, and Light Treatments
3. Discussion
4. Materials and Methods
4.1. Identification and Analysis of CAMTA Gene Families in P. bournei
4.2. Physical and Chemical Characteristics of P. bournei
4.3. Analysis of the Phylogenetic Tree and Motif, Domain, and Gene Structures of PbCAMTAs
4.4. Phylogenetic Analysis between PbCAMTAs and CAMTAs of Other Species
4.5. Collinearity Analysis and Duplication Events Analysis of CAMTA
4.6. GO and KEGG Analysis
4.7. Protein–Protein Interaction Network of PbCAMTAs
4.8. Abiotic Stress Treatment
4.9. RNA Extraction and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene ID | Size/aa | Molecular Weight/kDa | Theoretical PI | Grand Average of Hydropathicity | Instability Index | Subcellular Localization |
---|---|---|---|---|---|---|---|
PbCAMTA1 | OF24973 | 1050 | 117.00 | 5.95 | −0.538 | 40.02 | cytoplasm |
PbCAMTA2 | OF11451 | 1059 | 117.88 | 5.39 | −0.464 | 42.68 | nucleus |
PbCAMTA3 | OF06278 | 1157 | 129.66 | 5.83 | −0.553 | 44.52 | nucleus |
PbCAMTA4 | OF03012 | 964 | 108.86 | 6.59 | −0.377 | 82.89 | cytoplasm |
PbCAMTA5 | OF03004 | 720 | 79.77 | 5.27 | −0.255 | 34.69 | nucleus |
PbCAMTA6 | OF19359 | 1088 | 121.79 | 5.79 | −0.525 | 44.20 | nucleus |
PbCAMTA7 | OF07073 | 1112 | 124.82 | 5.82 | −0.561 | 45.34 | nucleus |
PbCAMTA8 | OF07605 | 982 | 110.74 | 8.42 | −0.518 | 36.32 | nucleus |
PbCAMTA9 | OF07600 | 532 | 36.37 | 5.68 | −0.520 | 47.73 | nucleus |
PbCAMTA10 | OF24143 | 340 | 55.07 | 4.46 | −0.564 | 39.56 | nucleus |
PbCAMTA11 | OF17261 | 350 | 38.75 | 6.04 | −0.498 | 60.49 | chloroplast |
PbCAMTA12 | OF00973 | 317 | 35.63 | 9.21 | −0.5115 | 40.97 | nucleus |
PbCAMTA13 | OF21192 | 514 | 55.07 | 7.02 | −0.058 | 39.45 | chloroplast |
PbCAMTA14 | OF20820 | 317 | 59.31 | 6.33 | −0.066 | 33.92 | nucleus |
PbCAMTA15 | OF09478 | 539 | 69.12 | 6.37 | −0.207 | 32.58 | Plasma memberane |
PbCAMTA16 | OF20135 | 635 | 70.07 | 6.59 | −0.219 | 31.69 | Plasma membrane |
PbCAMTA17 | OF14546 | 600 | 66.96 | 6.32 | −0.002 | 28.67 | nucleus |
Gene Name | Forward | Reverse |
---|---|---|
PbCAMTA1 | GAACCATGCCCATCGATCA | GATATTGCTGCCGTGCCTTG |
PbCAMTA2 | GGTAGAACTGTTGGGGAGGC | TCCAATAGCAGCGCCTTTGA |
PbCAMTA4 | AGGTACTTCGCTGGTTGGTG | GCCTCCAAAAGATCTGCCCA |
PbCAMTA6 | TGCTGGTTACCTTGCGGAAT | CGCTCTGCAACTGTTTGGAC |
PbCAMTA8 | CCGCCATCGAATGCATCAAG | CTGTAGCATCCGTCAGTCCC |
PbCAMTA12 | ACGCATACCTAATCTGGCCG | ACATTGTGTTTGCGCAGCTT |
PbCAMTA16 | AACGGCCATGTCTGTGTTGA | GGAACCCATCAGACTTGCCA |
PbCAMTA17 | CTGCCCTATTGGCTGGGAAT | GCTCAACACAACGATCGCAA |
EF1a | GCTCCTGGTCACCGTGAC | TCACGAGTTTGCCCGTCC |
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Zheng, K.; Li, M.; Yang, Z.; He, C.; Wu, Z.; Tong, Z.; Zhang, J.; Zhang, Y.; Cao, S. The Vital Role of the CAMTA Gene Family in Phoebe bournei in Response to Drought, Heat, and Light Stress. Int. J. Mol. Sci. 2024, 25, 9767. https://doi.org/10.3390/ijms25189767
Zheng K, Li M, Yang Z, He C, Wu Z, Tong Z, Zhang J, Zhang Y, Cao S. The Vital Role of the CAMTA Gene Family in Phoebe bournei in Response to Drought, Heat, and Light Stress. International Journal of Molecular Sciences. 2024; 25(18):9767. https://doi.org/10.3390/ijms25189767
Chicago/Turabian StyleZheng, Kehui, Min Li, Zhicheng Yang, Chenyue He, Zekai Wu, Zaikang Tong, Junhong Zhang, Yanzi Zhang, and Shijiang Cao. 2024. "The Vital Role of the CAMTA Gene Family in Phoebe bournei in Response to Drought, Heat, and Light Stress" International Journal of Molecular Sciences 25, no. 18: 9767. https://doi.org/10.3390/ijms25189767