Profiling Reduced Expression of Contractile and Mitochondrial mRNAs in the Human Sinoatrial Node vs. Right Atrium and Predicting Their Suppressed Expression by Transcription Factors and/or microRNAs
Abstract
:1. Introduction
2. Results
2.1. Differential mRNA Expression in the Human Sinus Node and Atrial Muscle
2.2. Differential TFs and miRs Expression in the Human SN and Atrial Muscle
2.3. Interactions between mRNAs and miRNAs, TFs
3. Discussion
4. Materials and Methods
4.1. Next-Generation Sequencing for mRNA, Bioinformatic Analysis for TFs and qPCR for miRs
4.2. Bioinformatic Analysis to Identify the mRNAs That Relate to Contractile Function, Mitochondrial Function and Glycogen Metabolism
4.2.1. RStudio Analysis
4.2.2. Canonical Pathway Analysis in IPA
4.2.3. Contractile Function, Mitochondrial Function, and Glycogen Metabolism Identification
4.3. Creating and Analysing mRNA, TF and miR Interactions/Networks in IPA
4.4. Binding Site Prediction between mRNAs and miRs
4.5. Heat Maps, Graphs, and Statistical Analysis
5. Limitations
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pathway (Number of Genes Involved) | Gene |
---|---|
TCA Cycle II (Eukaryotic) (7) | ACO2, CS, FH, IDH3A, MDH1, OGDH, SDHA |
Calcium Signaling (17) | ATP2A2, CAMK2A, CAMK2B, CASQ2, CHRNE, GRIN2A, GRIN2C, MYH6, MYH14, MYL4, PNCK, PRKAG2, PRKAR2B, RCAN2, RYR2, TNNT2, TRDN |
Oxidative Phosphorylation (12) | ATPAF1, COX10, COX5A, COX6A2, CYCS, NDUFA4, NDUFA5, NDUFAB1, NDUFS1, SDHA, UQCRB, UQCRC2 |
Gluconeogenesis I (6) | ALDOA, ENO3, MDH1, ME1, ME2, PGAM2 |
Apelin Muscle Signaling Pathway (4) | APLN, PRKAA2, PRKAG2, SLC2A4 |
Cardiac Hypertrophy Signaling (Enhanced) (22) | ATP2A2, CAMK2A, CAMK2B, HSPB3, HSPB7, IFNLR1, IL5RA, MAP3K9, MAP3K20, MAPKAPK3, NPPA, PDE3A, PDE7A, PDE8B, PDK1, PLCL1, PLN, PRKAG2, PRKAR2B, RASD1, RPS6KA5, RYR2 |
Dopamine-DARPP32 Feedback in cAMP Signaling (13) | ATP2A2, GRIN2A, GRIN2C, KCNJ11, PLCL1, PPM1L, PPP1R3A, PPP1R3C, PPP2R2C, PPP2R3A, PRKAG2, PRKAR2B, PRKG2 |
Protein Kinase A Signaling (23) | AKAP1, AKAP3, AKAP6, CAMK2A, CAMK2B, EYA1, MYL4, MYLK3, NTN1, PDE3A, PDE7A, PDE8B, PHKG1, PLCL1, PLN, PPP1R3A, PPP1R3C, PRKAG2, PRKAR2B, PTPRQ, PYGM, RYR2, TTN |
Factors Promoting Cardiogenesis in Vertebrates (11) | BMP7, BMP10, BMP8A, CAMK2A, CAMK2B, GJA5, MYH6, NPPA, PLCL1, SCN5A, TBX5 |
BMP signaling pathway (7) | BMP7, BMP8A, BMP10, PRKAG2, PRKAR2B, RASD1, SOSTDC1 |
Synaptic Long Term Potentiation (10) | CAMK2A, CAMK2B, GRIN2A, GRIN2C, PLCL1, PPP1R3A, PPP1R3C, PRKAG2, PRKAR2B, RASD1 |
Melatonin Signaling (7) | CAMK2A, CAMK2B, PLCL1, PRKAG2, PRKAR2B, RORC, SLC2A4 |
Neuropathic Pain Signaling In Dorsal Horn Neurons (8) | CAMK2A, CAMK2B, GRIN2A, GRIN2C, KCNH2, PLCL1, PRKAG2, PRKAR2B |
Insulin Secretion Signaling Pathway (14) | CAMK2A, CAMK2B, DLAT, GIPR, KCNJ11, NALCN, PDHA1, PLCL1, PRKAG2, PRKAR2B, RPS6KA5, RYR2, SLC2A4, STAT4 |
White Adipose Tissue Browning Pathway (8) | BMP7, FNDC5, NPPA, PRKAA2, PRKAG2, PRKAR2B, PRKG2, VEGFA |
Actin Cytoskeleton Signaling (13) | ACTN2, EGF, FGF11, FGF12, MYH6, MYH14, MYL4, MYLK3, PAK6, PIP5K1B, PPP1R12B, RASD1, TTN |
CDK5 Signaling (8) | PPM1L, PPP1R3A, PPP1R3C, PPP2R2C, PPP2R3A, PRKAG2, PRKAR2B, RASD1 |
cAMP-mediated signaling (13) | ADORA1, AKAP1, AKAP3, AKAP6, CAMK2A, CAMK2B, CHRM2, PDE3A, PDE7A, PDE8B, PKIA, PRKAR2B, RAP1GAP |
AMPK Signaling (11) | AK4, CHRM2, CHRNE, CKM, PPM1L, PPP2R2C, PPP2R3A, PRKAA2, PRKAG2, PRKAR2B, SLC2A4 |
ILK Signaling (11) | ACTN2, DSP, ITGB6, MYH6, MYH14, MYL4, PPM1L, PPP2R2C, PPP2R3A, RPS6KA5, VEGFA |
Nitric Oxide Signaling in the Cardiovascular System (7) | ATP2A2, PLN, PRKAG2, PRKAR2B, PRKG2, RYR2, VEGFA |
Superpathway of Methionine Degradation (4) | GOT1, GOT2, PCCB, PRMT8 |
eNOS Signaling (9) | AQP4, AQP7, CHRM2, CHRNE, LPAR3, PRKAA2, PRKAG2, PRKAR2B, VEGFA |
Apelin Pancreas Signaling Pathway (4) | APLN, PRKAA2, PRKAG2, PRKAR2B |
nNOS Signaling in Neurons (4) | CAMK2A, GRIN2A, GRIN2C, RASD1 |
P38 MAPK Signaling (6) | HSPB3, HSPB7, MAPKAPK3, PLA2G12B, RPS6KA5, TIFA |
ERK5 Signaling (3) | RASD1, RPS6KA5, WNK1 |
mRNA | SN Mean ± SEM | RA Mean ± SEM | Log2fold Change | Adjusted p Value |
---|---|---|---|---|
NPPA | 40,366.13 ± 11,840.28 | 587,003.64 ± 233,459.53 | −3.80 | 3.17 × 10−47 |
GRIN2C | 150.49 ± 41.54 | 1505.33 ± 206.64 | −3.40 | 1.32 × 10−29 |
GRIN2A | 34.41 ± 9.15 | 395.65 ± 63.35 | −3.61 | 1.20 × 10−21 |
LPAR3 | 239.30 ± 78.64 | 1563.00 ± 250.50 | −2.87 | 2.03 × 10−19 |
ENO3 | 947.30 ± 433.60 | 5786.00 ± 672.30 | −2.89 | 9.77 × 10−18 |
TMEM38B | 435.93 ± 66.81 | 2180.97 ± 137.96 | −2.37 | 5.96 × 10−17 |
FBXO32 | 1823.69 ± 622.47 | 8064.94 ± 2141.13 | −2.22 | 3.57 × 10−16 |
RYR2 | 6201.72 ± 2293.58 | 43,858.17 ± 21,127.23 | −2.77 | 1.82 × 10−15 |
CHRNE | 532.36 ± 146.12 | 2493.47 ± 302.70 | −2.33 | 2.01 × 10−14 |
MYLK3 | 2917.00 ± 966.30 | 16,247.00 ± 1172.00 | −2.65 | 5.87 × 10−14 |
PDE3A | 1669.00 ± 133.00 | 6510.00 ± 506.40 | −1.98 | 9.75 × 10−14 |
ME2 | 1040.19 ± 75.53 | 4288.91 ± 471.40 | −2.05 | 1.07 × 10−13 |
PPP1R12B | 8186.14 ± 2305.97 | 37,247.20 ± 6630.09 | −2.25 | 6.54 × 10−13 |
RAP1GAP | 107.50 ± 12.52 | 544.70 ± 21.50 | −2.37 | 7.62 × 10−13 |
KCNIP2 | 1871.96 ± 431.95 | 9718.33 ± 735.63 | −2.47 | 1.86 × 10−12 |
SCN1B | 458.62 ± 60.01 | 1785.74 ± 293.19 | −1.96 | 4.18 × 10−12 |
PDE8B | 506.40 ± 113.30 | 1798.00 ± 188.20 | −1.91 | 2.64 × 10−10 |
ATP2A2 | 32,967.84 ± 8525.27 | 140,042.55 ± 11,888.16 | −2.20 | 3.15 × 10−10 |
SGCD | 1118.50 ± 130.93 | 4005.43 ± 752.98 | −1.83 | 4.79 × 10−10 |
ANK1 | 326.65 ± 72.56 | 1302.83 ± 51.25 | −2.09 | 5.94 × 10−10 |
PDK1 | 435.31 ± 85.02 | 1691.11 ± 137.84 | −2.02 | 1.09 × 10−9 |
NEDD4L | 548.57 ± 131.29 | 2313.96 ± 493.04 | −2.13 | 1.11 × 10−9 |
UQCRB | 8496.29 ± 1355.26 | 31,581.55 ± 7034.10 | −1.85 | 1.68 × 10−9 |
PKIA | 957.30 ± 178.40 | 3342.00 ± 294.50 | −1.85 | 2.51 × 10−9 |
MYBPC1 | 80.81 ± 14.79 | 424.38 ± 67.97 | −2.41 | 4.76 × 10−9 |
TRIM63 | 1263.61 ± 398.03 | 5687.17 ± 1969.60 | −2.19 | 5.19 × 10−9 |
PRKAA2 | 952.70 ± 259.80 | 4127.00 ± 1218.00 | −2.16 | 5.44 × 10−9 |
ME1 | 645.39 ± 142.07 | 2306.13 ± 46.86 | −1.92 | 6.80 × 10−9 |
SLC39A8 | 726.23 ± 149.47 | 2476.79 ± 221.81 | −1.83 | 1.51 × 10−8 |
PRKAG2 | 3090.00 ± 572.40 | 106,57.00 ± 858.00 | −1.84 | 1.52 × 10−8 |
MYH14 | 1172.51 ± 230.82 | 3694.07 ± 614.29 | −1.69 | 1.72 × 10−8 |
DSG2 | 1180.31 ± 381.92 | 4273.57 ± 852.77 | −2.03 | 2.55 × 10−8 |
DMD | 2411.16 ± 277.11 | 8679.83 ± 1901.66 | −1.81 | 4.42 × 10−8 |
PRKAR2B | 791.19 ± 108.73 | 2418.93 ± 79.89 | −1.65 | 4.55 × 10−8 |
NDUFA5 | 2768.78 ± 345.70 | 8071.65 ± 861.85 | −1.56 | 5.13 × 10−8 |
DLAT | 1198.44 ± 194.96 | 3902.53 ± 187.84 | −1.75 | 5.18 × 10−8 |
TTN | 27,820.20 ± 8702.96 | 154,512.67 ± 72,399.52 | −2.38 | 5.39 × 10−8 |
CHRM2 | 1265.95 ± 525.92 | 3877.67 ± 1095.15 | −1.83 | 6.68 × 10−8 |
GOT1 | 4504.00 ± 1167.00 | 15,507.00 ± 642.50 | −1.90 | 7.20 × 10−8 |
MAP3K20 | 5446.00 ± 1082.00 | 16,464.00 ± 3505.00 | −1.60 | 9.15 × 10−8 |
AKAP1 | 1824.00 ± 512.30 | 5030.00 ± 656.60 | −1.56 | 1.23 × 10−7 |
TRDN | 2415.45 ± 701.59 | 10,011.69 ± 2499.98 | −2.15 | 1.65 × 10−7 |
ABLIM3 | 2310.55 ± 372.12 | 6165.94 ± 1169.24 | −1.42 | 1.95 × 10−7 |
KCNJ11 | 90.50 ± 26.67 | 370.59 ± 97.45 | −2.04 | 2.16 × 10−7 |
CYCS | 4494.10 ± 1257.61 | 14,511.32 ± 1868.14 | −1.78 | 3.26 × 10−7 |
IDH3A | 2512.14 ± 569.16 | 7355.81 ± 423.98 | −1.63 | 5.48 × 10−7 |
SDHA | 4783.13 ± 765.73 | 14,099.54 ± 1017.44 | −1.60 | 5.76 × 10−7 |
CAMK2B | 886.45 ± 242.81 | 2844.18 ± 389.22 | −1.81 | 6.27 × 10−7 |
ALDOA | 21,888.38 ± 4209.53 | 69,812.69 ± 10,672.80 | −1.71 | 6.44 × 10−7 |
COX10 | 370.51 ± 82.92 | 1177.80 ± 41.61 | −1.76 | 9.40 × 10−7 |
COX5A | 3850.64 ± 932.14 | 12,170.01 ± 2045.61 | −1.70 | 2.30 × 10−6 |
OGDH | 4144.09 ± 905.90 | 11,254.98 ± 1322.87 | −1.51 | 2.43 × 10−6 |
ATPAF1 | 2336.00 ± 496.90 | 6454.00 ± 15.82 | −1.55 | 2.71 × 10−6 |
NALCN | 200.50 ± 33.82 | 580.40 ± 38.73 | −1.58 | 2.88 × 10−6 |
PDE7A | 454.50 ± 93.88 | 1775.00 ± 711.30 | −1.80 | 2.91 × 10−6 |
ABLIM1 | 8707.20 ± 1976.33 | 23,835.54 ± 8276.75 | −1.39 | 3.61 × 10−6 |
PDHA1 | 4045.38 ± 917.17 | 11,743.03 ± 669.07 | −1.63 | 4.49 × 10−6 |
NDUFA4 | 11,401.81 ± 2311.16 | 34,441.74 ± 5244.19 | −1.63 | 5.67 × 10−6 |
PLN | 13,987.76 ± 3665.84 | 42,146.81 ± 4579.06 | −1.70 | 5.89 × 10−6 |
SLC25A4 | 14,584.36 ± 4389.42 | 46,121.40 ± 4482.91 | −1.80 | 5.98 × 10−6 |
UQCRC2 | 5516.37 ± 839.18 | 15,418.20 ± 1675.86 | −1.51 | 8.95 × 10−6 |
MDH1 | 9211.00 ± 2270.00 | 28,563.00 ± 4373.00 | −1.70 | 1.04 × 10−5 |
APLN | 53.12 ± 20.70 | 193.86 ± 52.80 | −2.08 | 1.16 × 10−5 |
AK4 | 954.20 ± 245.80 | 2598.00 ± 608.00 | −1.51 | 1.52 × 10−5 |
TRIM54 | 1293.40 ± 455.02 | 3632.34 ± 413.85 | −1.75 | 1.71 × 10−5 |
AKAP6 | 1628.06 ± 423.62 | 4406.32 ± 259.99 | −1.57 | 1.72 × 10−5 |
ANK3 | 2195.19 ± 135.79 | 5698.77 ± 854.85 | −1.36 | 2.24 × 10−5 |
IDH2 | 2813.86 ± 680.68 | 7679.94 ± 545.31 | −1.54 | 2.29 × 10−5 |
SLC2A4 | 1083.62 ± 376.68 | 3169.26 ± 913.33 | −1.63 | 2.40 × 10−5 |
ADORA1 | 202.32 ± 75.96 | 563.38 ± 53.83 | −1.72 | 3.06 × 10−5 |
NDUFAB1 | 2853.73 ± 666.19 | 8491.52 ± 1971.77 | −1.56 | 3.21 × 10−5 |
IMMT | 2548.38 ± 457.59 | 6293.75 ± 181.62 | −1.37 | 3.55 × 10−5 |
PRKACA | 3651.82 ± 592.69 | 8568.10 ± 302.59 | −1.28 | 3.68 × 10−5 |
AIFM1 | 1250.79 ± 272.32 | 3128.84 ± 75.60 | −1.41 | 6.67 × 10−5 |
TNNT2 | 52,996.68 ± 15,892.94 | 144,599.19 ± 21,446.60 | −1.56 | 1.10 × 10−4 |
SLC25A12 | 1658.49 ± 208.14 | 3801.41 ± 191.81 | −1.23 | 1.12 × 10−4 |
KLHL3 | 583.44 ± 155.63 | 1617.75 ± 772.65 | −1.28 | 1.14 × 10−4 |
TPM1 | 59,923.00 ± 20,212.99 | 153,480.87 ± 31,542.18 | −1.45 | 1.26 × 10−4 |
MRPL35 | 1054.26 ± 163.38 | 2374.22 ± 25.60 | −1.22 | 1.46 × 10−4 |
KCND3 | 409.54 ± 78.90 | 1311.74 ± 614.24 | −1.42 | 1.97 × 10−4 |
GPD1L | 4931.18 ± 1450.70 | 10,273.33 ± 1593.37 | −1.19 | 2.06 × 10−4 |
NDUFA8 | 1784.11 ± 388.01 | 4248.67 ± 601.70 | −1.30 | 2.44 × 10−4 |
NDUFB5 | 2470.69 ± 347.08 | 5857.23 ± 754.88 | −1.26 | 2.71 × 10−4 |
PDE4D | 934.41 ± 155.71 | 2037.22 ± 476.12 | −1.09 | 2.87 × 10−4 |
PRKAR1A | 7776.36 ± 550.02 | 15,506.23 ± 1274.24 | −1.01 | 3.71 × 10−4 |
CYC1 | 3470.36 ± 625.10 | 8433.44 ± 1428.91 | −1.29 | 4.35 × 10−4 |
EDNRB | 1215.00 ± 114.40 | 2583.00 ± 176.70 | −1.11 | 4.69 × 10−4 |
GYS1 | 1783.00 ± 630.40 | 3749.00 ± 805.40 | −1.21 | 5.40 × 10−4 |
MRPS23 | 674.46 ± 103.97 | 1396.07 ± 128.12 | −1.08 | 5.40 × 10−4 |
HIGD1A | 1351.36 ± 282.95 | 2904.10 ± 537.75 | −1.12 | 5.62 × 10−4 |
MRPL33 | 1310.67 ± 180.02 | 2891.31 ± 306.91 | −1.16 | 5.85 × 10−4 |
SLC25A11 | 1854.97 ± 336.97 | 4194.34 ± 509.14 | −1.21 | 9.34 × 10−4 |
MRPL39 | 449.21 ± 36.86 | 985.84 ± 149.48 | −1.12 | 9.68 × 10−4 |
NDUFA6 | 2675.74 ± 698.20 | 6039.42 ± 1259.69 | −1.20 | 1.04 × 10−3 |
COX5B | 6125.33 ± 1447.09 | 14,888.12 ± 3592.31 | −1.26 | 1.10 × 10−3 |
DLD | 2161.55 ± 418.65 | 4964.17 ± 623.10 | −1.25 | 1.20 × 10−3 |
MRPL50 | 540.20 ± 47.38 | 1071.58 ± 32.52 | −1.01 | 1.23 × 10−3 |
COX8A | 1709.46 ± 377.55 | 4097.35 ± 964.90 | −1.23 | 1.29 × 10−3 |
AFG3L2 | 1471.45 ± 191.94 | 2892.52 ± 40.73 | −1.01 | 1.30 × 10−3 |
CAMK2A | 226.40 ± 89.79 | 539.50 ± 31.36 | −1.54 | 1.43 × 10−3 |
COX6B1 | 4041.13 ± 1030.85 | 9143.47 ± 2151.20 | −1.18 | 1.55 × 10−3 |
MRPS22 | 683.43 ± 37.07 | 1432.12 ± 201.58 | −1.05 | 1.57 × 10−3 |
NDUFB10 | 3759.25 ± 798.24 | 8448.57 ± 1735.75 | −1.16 | 1.73 × 10−3 |
SLC25A3 | 10,661.69 ± 1643.67 | 22,876.10 ± 3179.36 | −1.12 | 2.08 × 10−3 |
NDUFS5 | 4811.13 ± 932.38 | 9909.52 ± 2177.33 | −1.02 | 2.92 × 10−3 |
PPIF | 1692.49 ± 512.50 | 3511.49 ± 61.05 | −1.24 | 3.34 × 10−3 |
MRPS33 | 721.80 ± 49.51 | 1461.57 ± 210.66 | −1.01 | 3.43 × 10−3 |
CRYAB | 42,557.08 ± 15,964.14 | 84,832.79 ± 15,603.49 | −1.13 | 3.44 × 10−3 |
SVIL | 6089.43 ± 1387.26 | 12,895.94 ± 3926.73 | −1.04 | 3.67 × 10−3 |
MRPS9 | 865.43 ± 123.52 | 1804.79 ± 260.93 | −1.07 | 4.47 × 10−3 |
DES | 112,042.00 ± 40,572.00 | 202,808.00 ± 17,832.00 | −1.14 | 7.18 × 10−3 |
MRPL15 | 1129.54 ± 234.79 | 2254.08 ± 289.96 | −1.04 | 7.61 × 10−3 |
TF (Number of Interactions) | mRNA Target |
---|---|
MYCN (29) | MRPL15, MRPL33, MRPS23, MRPS9, MRPL39, SDHA, MRPL35, NDUFS5, COX5B, COX5A, IDH2, NDUFB10, MRPL50, NDUFA6, MRPS33, SLC25A4, NDUFA5, DLAT, SLC25A11, MRPS22, UQCRC2, COX6B1, SLC25A3, NDUFAB1, NDUFA8, TTN, ATP2A2, CAMK2A, MYH14 |
SOX2 (14) | SLC25A12, MDH1, CRYAB, SLC25A11, MRPL39, MRPS9, MRPS23, MRPL15, AIFM1, ABLIM3, MYH14, PRKAR2B, PRKAR1A, PDE4D |
NUPR1 (7) | IMMT, DLD, PDHA1, OGDH, AIFM1, DSG2, SVIL |
PRDM16 (5) | CYC1, IMMT, NDUFA4, SLC25A11, ATP2A2 |
LMO2 (4) | NDUFA8, NDUFAB1, TRIM54, TRIM63 |
GLI1 (3) | DES, PRKAA2, PRKACA |
TBX3 (3) | SLC25A3, AFG3L2, SVIL |
VAV1 (3) | SLC25A11, SLC25A3, ABLIM1 |
HDAC10 (2) | NDUFB5, TRIM54 |
KCNIP3 (2) | KCNIP2, KCND3 |
PYCARD (2) | GOT1, PRKAR1A |
CEBPA (1) | SLC25A11 |
IKZF1 (1) | PRKAA2 |
TGFB1I1 (1) | NEDD4L |
ATF5 (1) | TNNT2 |
BCL11B (1) | MAP3K20 |
BATF (1) | COX8A |
DTX1 (1) | PRKAR1A |
HOXB3 (1) | SDHA |
LBH (1) | CRYAB |
MLXIPL (1) | PRKAA2 |
SHOX2 (1) | GRIN2C |
SOX9 (1) | KLHL3 |
TBX2 (1) | AFG3L2 |
TSC22D3 (1) | NEDD4L |
miRNA (Number of Interactions) | mRNA of Predicted Target | Number of Binding Sites for Each miRNA on Predicted Target (RNA22) | Number of Binding Sites for Each miRNA on Predicted Target (TargetScanHuman) |
---|---|---|---|
hsa-miR-153-3p (19) | ANK3 | 0 | 2 |
TRDN | 0 | 1 | |
DSG2 | 1 | 1 | |
PPP1R12B | 0 | 1 | |
DMD | 0 | 1 | |
KLHL3 | 1 | 3 | |
RYR2 | 0 | 2 | |
KCNIP2 | 0 | 1 | |
PDE7A | 0 | 2 | |
PRKAA2 | 1 | 1 | |
SGCD | 1 | 1 | |
AKAP6 | 0 | 1 | |
ABLIM1 | 1 | 1 | |
ANK1 | 0 | 1 | |
CHRM2 | 0 | 1 | |
GPD1L | 0 | 1 | |
FBXO32 | 1 | 1 | |
SLC39A8 | 0 | 1 | |
ME1 | 1 | 1 | |
hsa-miR-654-5p (15) | TRIM54 | 1 | 0 |
MYBPC1 | 1 | 0 | |
CAMK2A | 3 | 0 | |
CHRNE | 1 | 0 | |
KCNIP2 | 1 | 0 | |
KCNJ11 | 2 | 0 | |
APLN | 5 | 0 | |
GRIN2A | 8 | 0 | |
ADORA1 | 1 | 0 | |
ANK1 | 2 | 0 | |
PRKACA | 1 | 0 | |
OGDH | 4 | 0 | |
ATPAF1 | 4 | 0 | |
ME2 | 4 | 0 | |
PPIF | 4 | 0 | |
hsa-miR-10a-5p (13) | PRKAA2 | 0 | 2 |
PDE7A | 0 | 2 | |
PDE8B | 0 | 3 | |
CAMK2B | 0 | 2 | |
TMEM38B | 0 | 4 | |
PRKACA | 0 | 1 | |
LPAR3 | 0 | 2 | |
ANK1 | 0 | 2 | |
ABLIM1 | 0 | 1 | |
SGCD | 0 | 1 | |
IDH3A | 0 | 1 | |
RAP1GAP | 4 | 1 | |
AK4 | 0 | 7 | |
hsa-miR-204-5p (9) | AKAP1 | 1 | 2 |
CHRM2 | 0 | 7 | |
PDE3A | 1 | 2 | |
PKIA | 0 | 2 | |
MRPS33 | 2 | 0 | |
AK4 | 2 | 4 | |
COX10 | 0 | 1 | |
PDHA1 | 0 | 1 | |
COX5A | 0 | 2 | |
hsa-miR-215-5p (9) | PLN | 0 | 2 |
LPAR3 | 0 | 2 | |
GRIN2A | 2 | 2 | |
SGCD | 0 | 2 | |
PRKAR1A | 0 | 2 | |
APLN | 0 | 1 | |
GOT1 | 0 | 2 | |
HIGD1A | 0 | 2 | |
CYCS | 1 | 3 | |
hsa-miR-193b-3p (6) | MYLK3 | 0 | 3 |
ANK3 | 0 | 2 | |
APLN | 0 | 1 | |
KCNJ11 | 0 | 1 | |
SGCD | 0 | 2 | |
PPIF | 0 | 2 | |
hsa-miR-885-3p (5) | SCN1B | 3 | 0 |
TPM1 | 2 | 0 | |
ADORA1 | 5 | 0 | |
COX10 | 3 | 0 | |
OGDH | 3 | 0 | |
hsa-miR-668-3p (4) | TTN | 1 | 1 |
NALCN | 2 | 0 | |
PDK1 | 4 | 0 | |
AK4 | 4 | 1 | |
hsa-miR-548b-5p (3) | TPM1 | 1 | 0 |
HIGD1A | 2 | 0 | |
UQCRB | 10 | 0 | |
hsa-miR-512-5p (3) | CAMK2B | 2 | 0 |
CYCS | 2 | 0 | |
MRPL50 | 1 | 0 | |
hsa-miR-377-5p (3) | EDNRB | 1 | 0 |
ANK1 | 3 | 0 | |
NPPA | 1 | 0 | |
hsa-miR-1225-3p (2) | CAMK2B | 2 | 0 |
PRKACA | 2 | 0 | |
hsa-miR-1244 (2) | GPD1L | 1 | 0 |
TMEM38B | 1 | 0 | |
hsa-miR-10b-3P (2) | GRIN2A | 2 | 0 |
MRPL35 | 1 | 0 | |
hsa-miR-198 (1) | RYR2 | 1 | 0 |
hsa-miR-548k (1) | PDK1 | 2 | 0 |
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Chen, W.; Aminu, A.J.; Yin, Z.; Karaesmen, I.; Atkinson, A.J.; Kuniewicz, M.; Holda, M.; Walocha, J.; Perde, F.; Molenaar, P.; et al. Profiling Reduced Expression of Contractile and Mitochondrial mRNAs in the Human Sinoatrial Node vs. Right Atrium and Predicting Their Suppressed Expression by Transcription Factors and/or microRNAs. Int. J. Mol. Sci. 2024, 25, 10402. https://doi.org/10.3390/ijms251910402
Chen W, Aminu AJ, Yin Z, Karaesmen I, Atkinson AJ, Kuniewicz M, Holda M, Walocha J, Perde F, Molenaar P, et al. Profiling Reduced Expression of Contractile and Mitochondrial mRNAs in the Human Sinoatrial Node vs. Right Atrium and Predicting Their Suppressed Expression by Transcription Factors and/or microRNAs. International Journal of Molecular Sciences. 2024; 25(19):10402. https://doi.org/10.3390/ijms251910402
Chicago/Turabian StyleChen, Weixuan, Abimbola J. Aminu, Zeyuan Yin, Irem Karaesmen, Andrew J. Atkinson, Marcin Kuniewicz, Mateusz Holda, Jerzy Walocha, Filip Perde, Peter Molenaar, and et al. 2024. "Profiling Reduced Expression of Contractile and Mitochondrial mRNAs in the Human Sinoatrial Node vs. Right Atrium and Predicting Their Suppressed Expression by Transcription Factors and/or microRNAs" International Journal of Molecular Sciences 25, no. 19: 10402. https://doi.org/10.3390/ijms251910402