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Article

Comparative Metabolome and Transcriptome Analysis Reveals the Defense Mechanism of Chinese Cabbage (Brassica rapa L. ssp. pekinensis) against Plasmodiophora brassicae Infection

by
Xiaochun Wei
1,2,†,
Yingyi Du
1,2,†,
Wenjing Zhang
1,
Yanyan Zhao
1,
Shuangjuan Yang
1,
Henan Su
1,
Zhiyong Wang
1,
Fang Wei
1,2,
Baoming Tian
1,2,
Haohui Yang
1,
Xiaowei Zhang
1,* and
Yuxiang Yuan
1,*
1
Institute of Vegetables, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, China
2
School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(19), 10440; https://doi.org/10.3390/ijms251910440
Submission received: 27 August 2024 / Revised: 24 September 2024 / Accepted: 25 September 2024 / Published: 27 September 2024
(This article belongs to the Special Issue Advances in Brassica Crop Metabolism and Genetics)

Abstract

Chinese cabbage (Brassica rapa L. ssp. pekinensis) ranks among the most cultivated and consumed vegetables in China. A major threat to its production is Plasmodiophora brassicae, which causes large root tumors, obstructing nutrient and water absorption and resulting in plant withering. This study used a widely targeted metabolome technique to identify resistance-related metabolites in resistant (DH40R) and susceptible (DH199S) Chinese cabbage varieties after inoculation with P. brassicae. This study analyzed disease-related metabolites during different periods, identifying 257 metabolites linked to resistance, enriched in the phenylpropanoid biosynthesis pathway, and 248 metabolites linked to susceptibility, enriched in the arachidonic acid metabolism pathway. Key metabolites and genes in the phenylpropanoid pathway were upregulated at 5 days post-inoculation (DPI), suggesting their role in disease resistance. In the arachidonic acid pathway, linoleic acid and gamma-linolenic acid were upregulated at 5 and 22 DPI in resistant plants, while arachidonic acid was upregulated at 22 DPI in susceptible plants, leading to the conclusion that arachidonic acid may be a response substance in susceptible plants after inoculation. Many genes enriched in these pathways were differentially expressed in DH40R and DH199S. The research provided insights into the defense mechanisms of Chinese cabbage against P. brassicae through combined metabolome and transcriptome analysis.
Keywords: Chinese cabbage; metabolome; transcriptome; Plasmodiophora brassicae; phenylpropanoid; arachidonic acid Chinese cabbage; metabolome; transcriptome; Plasmodiophora brassicae; phenylpropanoid; arachidonic acid

Share and Cite

MDPI and ACS Style

Wei, X.; Du, Y.; Zhang, W.; Zhao, Y.; Yang, S.; Su, H.; Wang, Z.; Wei, F.; Tian, B.; Yang, H.; et al. Comparative Metabolome and Transcriptome Analysis Reveals the Defense Mechanism of Chinese Cabbage (Brassica rapa L. ssp. pekinensis) against Plasmodiophora brassicae Infection. Int. J. Mol. Sci. 2024, 25, 10440. https://doi.org/10.3390/ijms251910440

AMA Style

Wei X, Du Y, Zhang W, Zhao Y, Yang S, Su H, Wang Z, Wei F, Tian B, Yang H, et al. Comparative Metabolome and Transcriptome Analysis Reveals the Defense Mechanism of Chinese Cabbage (Brassica rapa L. ssp. pekinensis) against Plasmodiophora brassicae Infection. International Journal of Molecular Sciences. 2024; 25(19):10440. https://doi.org/10.3390/ijms251910440

Chicago/Turabian Style

Wei, Xiaochun, Yingyi Du, Wenjing Zhang, Yanyan Zhao, Shuangjuan Yang, Henan Su, Zhiyong Wang, Fang Wei, Baoming Tian, Haohui Yang, and et al. 2024. "Comparative Metabolome and Transcriptome Analysis Reveals the Defense Mechanism of Chinese Cabbage (Brassica rapa L. ssp. pekinensis) against Plasmodiophora brassicae Infection" International Journal of Molecular Sciences 25, no. 19: 10440. https://doi.org/10.3390/ijms251910440

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