Bioinformatic Analysis from a Descriptive Profile of miRNAs in Chronic Migraine
Abstract
:1. Introduction
2. Results
2.1. miRs Profile Expression in Patients with Chronic Migraine
2.2. miR-Targets Interaction in Patients with Chronic Migraine
2.3. Signalling Pathways in miR-Targets Profile in Chronic Migraine
2.4. Relationship among Chronic Migraine miRs and Other Pathologies
3. Discussion
4. Materials and Methods
4.1. Patient Selection and Blood Sample Collection
4.2. Extraction and Reverse Transcription of MicroRNAs from Blood Plasma
4.3. MicroRNA Expression
4.4. Bioinformatic Analysis of the Results
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Associated Conditions | Regulator miRs |
---|---|---|
PHLDA1 | Neuroinflammation [11], Drug resistance [12] | 197-3p, 101-3p, 92-3p, 375, 146b-3p |
ATXN1 | Spinocerebellar ataxia type 1 [13] Inflammation via NF-κB regulation [14] | 197-3p, 101-3p, 92-3p, 375, 146b-3p |
PPARGC1B | Gouty inflammation by NLRP3-inflammasome [15] | 197-3p, 101-3p, 92-3p, 375, 146b-3p |
ABI2 | Tumor promotion and invasion [16,17] | 197-3p, 101-3p, 92-3p, 375, 146b-3p |
LDLRAD4 | Metastasis [18], Intracerebral hemorrhage [19] | 197-3p, 101-3p, 92-3p, 375, 146b-3p |
NUFIP2 | Lysosomal damage [20], Neuroinflammation [21] | 197-3p, 101-3p, 92-3p, 375, 146b-3p |
TRIM44 | Inflammation [22] | 133a, 133b, 134, 195-3p, 340 |
miRs | Pathway | Hits (P) |
---|---|---|
hsa-miR-197-3p | Nervous system development | 970 (6.45 × 10−6) |
Neuronal differentiation | 552 (0.001) | |
Neuronal projection | 477 (0.001) | |
Neuronal system | 191 (0.002) | |
Neurogenesis | 645 (0.002) | |
hsa-miR-101-3p | Nervous system development | 950 (9.43 × 10−7) |
Neuronal differentiation | 441 (1.22 × 10−4) | |
Neuronal projection | 208 (9.80 × 10−6) | |
Neurogenesis | 645 (1.67 × 10−5) | |
Immune system processes | 100 (1.12 × 10−5) | |
Central nervous system vasculogenesis | 2 (0.023) | |
hsa-miR-92a-3p | Nervous system development | 883 (4.12 × 10−5) |
Neuronal differentiation | 509 (2.87 × 10−4) | |
Neuronal projection | 430 (0.010) | |
Neurogenesis | 609 (4.23 × 10−5) | |
Immune system processes | 258 (0.023) | |
Blood vessel development | 277 (0.009) | |
hsa-miR-375-3p | Nervous system development | 792 (3.23 × 10−7) |
Neuronal differentiation | 441 (9.59 × 10−4) | |
Neurogenesis | 523 (5.37 × 10−4) | |
Blood vessel development | 239 (0.025) | |
hsa-miR-146b-3p | Nervous system development | 886 (1.36 × 10−4) |
Neuronal differentiation | 513 (4.34 × 10−4) | |
Neuronal projection | 456 (7.68 × 10−5) | |
Neurogenesis | 612 (1.36 × 10−4) | |
Blood vessel morphogenesis | 242 (0.028) | |
hsa-miR-133a-3p | Nervous system development | 745 (1.01 × 10−7) |
Neuronal differentiation | 424 (6.55 × 10−5) | |
Neuronal projection | 360 (9.13 × 10−4) | |
Neurogenesis | 502 (1.89 × 10−5) | |
Immune system development | 19 (0.003) | |
Immune system processes | 34 (0.009) | |
Vascular development | 12 (0.031) | |
Blood vessel morphogenesis | 12 (0.025) | |
hsa-miR-133b | Nervous system development | 745 (1.01 × 10−7) |
Neuronal differentiation | 424 (6.55 × 10−5) | |
Neuronal projection | 360 (9.13 × 10−4) | |
Neurogenesis | 14 (0.026) | |
Immune system development | 12 (0.007) | |
Immune system processes | 22 (0.021) | |
Vascular development | 12 (9.29 × 10−4) | |
Blood vessel development | 12 (7.13 × 10−4) | |
hsa-miR-134-5p | Nervous system development | 773 (0.002) |
Neuronal projection | 402 (1.77 × 10−4) | |
Neurogenesis | 512 (0.036) | |
Vascular development | 261 (0.007) | |
Blood vessel development | 250 (0.011) | |
Blood vessel morphogenesis | 223 (0.009) | |
hsa-miR-195-3p | Nervous system development | 959 (2.50 × 10−11) |
Neuronal differentiation | 544 (3.35 × 10−7) | |
Neuronal projection | 445 (0.010) | |
Neurogenesis | 637 (5.54 × 10−7) | |
Immune system development | 380 (0.009) | |
Blood vessel development | 304 (7.85 × 10−6) | |
Blood vessel morphogenesis | 267 (3.10 × 10−5) | |
hsa-miR-340-5p | Nervous system development | 701 (1.02 × 10−4) |
Neuronal differentiation | 695 (6.98 × 10−7) | |
Neurogenesis | 826 (1.10 × 10−7) | |
Immune system development | 505 (5.11 × 10−4) | |
Cytokine signaling | 8 (0.009) | |
Vascular development | 226 (0.027) |
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Tovar-Cuevas, A.J.; Rosales Gómez, R.C.; Martín-Márquez, B.T.; Peña Dueñas, N.A.; Sandoval-García, F.; Guzmán Ornelas, M.O.; Chávez Tostado, M.; Hernández Corona, D.M.; Corona Meraz, F.-I. Bioinformatic Analysis from a Descriptive Profile of miRNAs in Chronic Migraine. Int. J. Mol. Sci. 2024, 25, 10491. https://doi.org/10.3390/ijms251910491
Tovar-Cuevas AJ, Rosales Gómez RC, Martín-Márquez BT, Peña Dueñas NA, Sandoval-García F, Guzmán Ornelas MO, Chávez Tostado M, Hernández Corona DM, Corona Meraz F-I. Bioinformatic Analysis from a Descriptive Profile of miRNAs in Chronic Migraine. International Journal of Molecular Sciences. 2024; 25(19):10491. https://doi.org/10.3390/ijms251910491
Chicago/Turabian StyleTovar-Cuevas, Alvaro Jovanny, Roberto Carlos Rosales Gómez, Beatriz Teresita Martín-Márquez, Nathan Alejandro Peña Dueñas, Flavio Sandoval-García, Milton Omar Guzmán Ornelas, Mariana Chávez Tostado, Diana Mercedes Hernández Corona, and Fernanda-Isadora Corona Meraz. 2024. "Bioinformatic Analysis from a Descriptive Profile of miRNAs in Chronic Migraine" International Journal of Molecular Sciences 25, no. 19: 10491. https://doi.org/10.3390/ijms251910491