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Open AccessArticle
Contribution of the Mobilome to the Configuration of the Resistome of Corynebacterium striatum
by
Catherine Urrutia
Catherine Urrutia 1,2,
Benjamin Leyton-Carcaman
Benjamin Leyton-Carcaman 1,3 and
Michel Abanto Marin
Michel Abanto Marin
Dr. Michel Abanto holds a BSc in Microbiology and Parasitology from the Universidad Nacional de in a [...]
Dr. Michel Abanto holds a BSc in Microbiology and Parasitology from the Universidad Nacional de Trujillo in Peru, an MSc in Molecular Biology from the Universidad Nacional Mayor de San Marcos in Peru, and a PhD in Computational and Systems Biology from the Oswaldo Cruz Institute in Brazil (2013). He then undertook a postdoctoral period in Microbial Genomics at the Oswaldo Cruz Institute (2013-2016), before taking up a position as a Research Associate in the Department of Biology and Biochemistry at the University of Bath in the UK. He currently holds an academic position at the Scientific and Technological Nucleus of Biological Resources (BIOREN) at the University of La Frontera in Chile. He is also the Head of the Genomics and Bioinformatics Unit at BIOREN. He has extensive experience in microbial genomics, metagenomics and bioinformatics. Dr. Abanto has worked with genomic data from important clinical and environmental bacterial species. He has also worked with functional genomics using RNA-seq in Atlantic salmon and human cancer. His research interests are microbial genomics, genome plasticity and applied bioinformatics. He has supervised undergraduate and graduate students for the past few years, as well as taught classes in microbial genomics and bioinformatics.
1,*
1
Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811230, Chile
2
Carrera de Biotecnología, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile
3
Doctorado en Ciencias Mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, Temuco 4811230, Chile
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(19), 10499; https://doi.org/10.3390/ijms251910499 (registering DOI)
Submission received: 8 September 2024
/
Revised: 26 September 2024
/
Accepted: 27 September 2024
/
Published: 29 September 2024
Abstract
Corynebacterium striatum, present in the microbiota of human skin and nasal mucosa, has recently emerged as a causative agent of hospital-acquired infections, notable for its resistance to multiple antimicrobials. Its mobilome comprises several mobile genetic elements, such as plasmids, transposons, insertion sequences and integrons, which contribute to the acquisition of antimicrobial resistance genes. This study analyzes the contribution of the C. striatum mobilome in the transfer and dissemination of resistance genes. In addition, integrative and conjugative elements (ICEs), essential in the dissemination of resistance genes between bacterial populations, whose role in C. striatum has not yet been studied, are examined. This study examined 365 C. striatum genomes obtained from the NCBI Pathogen Detection database. Phylogenetic and pangenome analyses were performed, the resistance profile of the bacterium was recognized, and mobile elements, including putative ICE, were detected. Bioinformatic analyses identified 20 antimicrobial resistance genes in this species, with the Ermx gene being the most predominant. Resistance genes were mainly associated with plasmid sequence regions and class 1 integrons. Although an ICE was detected, no resistance genes linked to this element were found. This study provided valuable information on the geographic spread and prevalence of outbreaks observed through phylogenetic and pangenome analyses, along with identifying antimicrobial resistance genes and mobile genetic elements that carry many of the resistance genes and may be the subject of future research and therapeutic approaches.
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MDPI and ACS Style
Urrutia, C.; Leyton-Carcaman, B.; Marin, M.A.
Contribution of the Mobilome to the Configuration of the Resistome of Corynebacterium striatum. Int. J. Mol. Sci. 2024, 25, 10499.
https://doi.org/10.3390/ijms251910499
AMA Style
Urrutia C, Leyton-Carcaman B, Marin MA.
Contribution of the Mobilome to the Configuration of the Resistome of Corynebacterium striatum. International Journal of Molecular Sciences. 2024; 25(19):10499.
https://doi.org/10.3390/ijms251910499
Chicago/Turabian Style
Urrutia, Catherine, Benjamin Leyton-Carcaman, and Michel Abanto Marin.
2024. "Contribution of the Mobilome to the Configuration of the Resistome of Corynebacterium striatum" International Journal of Molecular Sciences 25, no. 19: 10499.
https://doi.org/10.3390/ijms251910499
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