Modulation of the Oncogenic LINE-1 Regulatory Network in Non-Small Cell Lung Cancer by Exosomal miRNAs
Abstract
:1. Introduction
2. Results
2.1. Exosome Characterization
2.2. Predicted Interactions between Genes within the LINE-1 Regulatory Network and Exosomal miRNAs
2.3. miRNA-Regulated Oncogenic Signaling in Human Lung Bronchial Epithelial Cells
3. Discussion
4. Material and Methods
4.1. Identification of miRNA-Regulated Targets within the LINE-1 Genetic Regulatory Network
4.2. Exosome Isolation and Characterization
4.3. Cell Culture
4.4. Co-Culture Experiments
4.5. RNA Isolation, cDNA Synthesis and Realtime-PCR
4.6. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hassanin, A.A.I.; Ramos, K.S. Modulation of the Oncogenic LINE-1 Regulatory Network in Non-Small Cell Lung Cancer by Exosomal miRNAs. Int. J. Mol. Sci. 2024, 25, 10674. https://doi.org/10.3390/ijms251910674
Hassanin AAI, Ramos KS. Modulation of the Oncogenic LINE-1 Regulatory Network in Non-Small Cell Lung Cancer by Exosomal miRNAs. International Journal of Molecular Sciences. 2024; 25(19):10674. https://doi.org/10.3390/ijms251910674
Chicago/Turabian StyleHassanin, Abeer A. I., and Kenneth S. Ramos. 2024. "Modulation of the Oncogenic LINE-1 Regulatory Network in Non-Small Cell Lung Cancer by Exosomal miRNAs" International Journal of Molecular Sciences 25, no. 19: 10674. https://doi.org/10.3390/ijms251910674