Insights into the Hormone-Regulating Mechanism of Adventitious Root Formation in Softwood Cuttings of Cyclocarya paliurus and Optimization of the Hormone-Based Formula for Promoting Rooting
Abstract
:1. Introduction
2. Results
2.1. Variations in the Rooting Process and Related Rooting Parameters
2.2. Dynamics of Endogenous Hormones during the AR Formation Process
2.3. Overall Assessment of Transcriptome Profiles during the AR Formation Process
2.4. DEGs Involved in Phytohormone Signal Transduction Pathways
2.5. Co-Expression Network Analysis of Weight Genes
2.6. qRT-PCR Verification for Selected Genes
3. Discussion
3.1. Effects of Different Hormone-Based Formulas on Rooting Efficiency
3.2. Relationship between Endogenous Hormones and AR Formation
3.3. Regulatory Mechanisms of Endogenous Hormones during the AR Formation Process
4. Materials and Methods
4.1. Plant Materials
4.2. Experimental Design and Treatment Description
4.3. Rooting Process Observation and Sampling
4.4. Assessment of the Rooting Rate and Rooting Quality Indexes
4.5. Measurement of Endogenous Hormones
4.6. RNA Extraction and Transcriptomics Analysis
4.7. Quantitative Real-Time (qRT-PCR) Validation
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Tian, Y.; Yang, W.; Wan, S.; Fang, S. Insights into the Hormone-Regulating Mechanism of Adventitious Root Formation in Softwood Cuttings of Cyclocarya paliurus and Optimization of the Hormone-Based Formula for Promoting Rooting. Int. J. Mol. Sci. 2024, 25, 1343. https://doi.org/10.3390/ijms25021343
Tian Y, Yang W, Wan S, Fang S. Insights into the Hormone-Regulating Mechanism of Adventitious Root Formation in Softwood Cuttings of Cyclocarya paliurus and Optimization of the Hormone-Based Formula for Promoting Rooting. International Journal of Molecular Sciences. 2024; 25(2):1343. https://doi.org/10.3390/ijms25021343
Chicago/Turabian StyleTian, Yuan, Wanxia Yang, Shiying Wan, and Shengzuo Fang. 2024. "Insights into the Hormone-Regulating Mechanism of Adventitious Root Formation in Softwood Cuttings of Cyclocarya paliurus and Optimization of the Hormone-Based Formula for Promoting Rooting" International Journal of Molecular Sciences 25, no. 2: 1343. https://doi.org/10.3390/ijms25021343