Genome-Wide Analysis of Nuclear factor-YC Genes in the Tea Plant (Camellia sinensis) and Functional Identification of CsNF-YC6
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of CsNF-YC Subunits in the Tea Plant Genome
2.2. Expression Patterns of CsNF-YC Genes in Different Tissues and Their Responses to Abiotic Stresses
2.3. Morphological Characteristics of CsNF-YC6 Transgenic Arabidopsis Plants
2.4. Overexpression of CsNF-YC6 Enhances Stress Tolerance to Abiotic Factors
3. Discussion
3.1. Evolutionary Analysis and Molecular Characterization of the CsNF-YC Gene
3.2. CsNF-YC Genes Play an Important Role in Tea Plant Growth and Development and Response to Abiotic Stresses
3.3. Early Flowering and Improved Stress Tolerance by Overexpression of CsNF-YC6
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Treatments
4.2. Identification, Phylogenetic Tree Construction, Gene Structure, Motif Analysis and Multiple Comparisons
4.3. Quantitative Real-Time PCR
4.4. Cloning and Physicochemical Property Analysis of CsNF-YC Gene
4.5. Expression Vector Construction and Agrobacterium-Mediated Transformation of Arabidopsis thaliana
4.6. Abiotic Stresses Treatment
4.7. Morphological Characteristics of Transgenic Arabidopsis Plants
4.8. Measurement of Physiological Indicators
4.9. Experimental Data Processing and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chen, S.; Wei, X.; Hu, X.; Zhang, P.; Chang, K.; Zhang, D.; Chen, W.; Tang, D.; Tang, Q.; Li, P.; et al. Genome-Wide Analysis of Nuclear factor-YC Genes in the Tea Plant (Camellia sinensis) and Functional Identification of CsNF-YC6. Int. J. Mol. Sci. 2024, 25, 836. https://doi.org/10.3390/ijms25020836
Chen S, Wei X, Hu X, Zhang P, Chang K, Zhang D, Chen W, Tang D, Tang Q, Li P, et al. Genome-Wide Analysis of Nuclear factor-YC Genes in the Tea Plant (Camellia sinensis) and Functional Identification of CsNF-YC6. International Journal of Molecular Sciences. 2024; 25(2):836. https://doi.org/10.3390/ijms25020836
Chicago/Turabian StyleChen, Shengxiang, Xujiao Wei, Xiaoli Hu, Peng Zhang, Kailin Chang, Dongyang Zhang, Wei Chen, Dandan Tang, Qian Tang, Pinwu Li, and et al. 2024. "Genome-Wide Analysis of Nuclear factor-YC Genes in the Tea Plant (Camellia sinensis) and Functional Identification of CsNF-YC6" International Journal of Molecular Sciences 25, no. 2: 836. https://doi.org/10.3390/ijms25020836