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Volume 25
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10.3390/ijms252111701
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Article

Serum Lipoprotein Profiling by NMR Spectroscopy Reveals Alterations in HDL-1 and HDL-2 Apo-A2 Subfractions in Alzheimer’s Disease

by
Jonas Ellegaard Mortensen
1,2,
Trygve Andreassen
3,4,
Dorte Aalund Olsen
2,5,
Karsten Vestergaard
6,
Jonna Skov Madsen
2,5,
Søren Risom Kristensen
1,7 and
Shona Pedersen
8,*
1
Department of Clinical Biochemistry, Aalborg University Hospital, 9000 Aalborg, Denmark
2
Department of Biochemistry and Immunology, Lillebaelt Hospital, University Hospital of Southern Denmark, 7100 Vejle, Denmark
3
Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, 7491 Trondheim, Norway
4
Central Staff, St. Olavs Hospital HF, 7006 Trondheim, Norway
5
Department of Regional Health Research, Faculty of Health Sciences, University of Southern Denmark, 5230 Odense, Denmark
6
Department of Neurology, Aalborg University Hospital, 9000 Aalborg, Denmark
7
Department of Clinical Medicine, Aalborg University Hospital, 9000 Aalborg, Denmark
8
Department of Basic Medical Science, College of Medicine, Qatar University, QU Health, Doha 2713, Qatar
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(21), 11701; https://doi.org/10.3390/ijms252111701
Submission received: 10 October 2024 / Revised: 28 October 2024 / Accepted: 30 October 2024 / Published: 31 October 2024
(This article belongs to the Special Issue Omics Era: From Principles to Applications in Disease Diagnosis and Treatment)
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Abstract

Identifying biomarkers for Alzheimer’s disease (AD) is crucial, due to its complex pathology, which involves dysfunction in lipid transport, contributing to neuroinflammation, synaptic loss, and impaired amyloid-β clearance. Nuclear magnetic resonance (NMR) is able to quantify and stratify lipoproteins. The study investigated lipoproteins in blood from AD patients, aiming to evaluate their diagnostic potential. Serum and plasma were collected from AD patients (n = 25) and healthy individuals (n = 25). We conducted a comprehensive lipoprotein profiling on serum samples using NMR spectroscopy, analysing 112 lipoprotein subfractions. In plasma, we measured unspecific markers of neuronal damage and AD hallmark proteins using single molecule array technology. Additionally, clinical data and cerebrospinal fluid biomarker levels were also collected to enrich our data. Our findings, after adjusting for age and sex differences, highlight significant alterations in two specific lipoproteins; high-density lipoprotein (HDL)-1 Apo-A2 (H1A2) and HDL-2 Apo-A2 (H2A2), both with area under the curve (AUC) values of 0.67, 95% confidence interval (CI) = 0.52–0.82). These results indicate that these lipoprotein subfractions may have potential as indicators of AD-related metabolic changes.
Keywords: Alzheimer’s disease; lipoproteins; blood; serum; nuclear magnetic resonance; biomarker Alzheimer’s disease; lipoproteins; blood; serum; nuclear magnetic resonance; biomarker

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MDPI and ACS Style

Mortensen, J.E.; Andreassen, T.; Olsen, D.A.; Vestergaard, K.; Madsen, J.S.; Kristensen, S.R.; Pedersen, S. Serum Lipoprotein Profiling by NMR Spectroscopy Reveals Alterations in HDL-1 and HDL-2 Apo-A2 Subfractions in Alzheimer’s Disease. Int. J. Mol. Sci. 2024, 25, 11701. https://doi.org/10.3390/ijms252111701

AMA Style

Mortensen JE, Andreassen T, Olsen DA, Vestergaard K, Madsen JS, Kristensen SR, Pedersen S. Serum Lipoprotein Profiling by NMR Spectroscopy Reveals Alterations in HDL-1 and HDL-2 Apo-A2 Subfractions in Alzheimer’s Disease. International Journal of Molecular Sciences. 2024; 25(21):11701. https://doi.org/10.3390/ijms252111701

Chicago/Turabian Style

Mortensen, Jonas Ellegaard, Trygve Andreassen, Dorte Aalund Olsen, Karsten Vestergaard, Jonna Skov Madsen, Søren Risom Kristensen, and Shona Pedersen. 2024. "Serum Lipoprotein Profiling by NMR Spectroscopy Reveals Alterations in HDL-1 and HDL-2 Apo-A2 Subfractions in Alzheimer’s Disease" International Journal of Molecular Sciences 25, no. 21: 11701. https://doi.org/10.3390/ijms252111701

APA Style

Mortensen, J. E., Andreassen, T., Olsen, D. A., Vestergaard, K., Madsen, J. S., Kristensen, S. R., & Pedersen, S. (2024). Serum Lipoprotein Profiling by NMR Spectroscopy Reveals Alterations in HDL-1 and HDL-2 Apo-A2 Subfractions in Alzheimer’s Disease. International Journal of Molecular Sciences, 25(21), 11701. https://doi.org/10.3390/ijms252111701

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