Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain

Huang, Zhenkuai; Peng, Zijing; Zhang, Mengli; Li, Xinhai; Qiu, Xiaoting

doi:10.3390/ijms252111774

Open AccessReview

Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain

by

Zhenkuai Huang

,

Zijing Peng

,

Mengli Zhang

,

Xinhai Li

and

Xiaoting Qiu

^*

College of Food Science and Engineering, Ningbo University, Ningbo 315800, China

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2024, 25(21), 11774; https://doi.org/10.3390/ijms252111774

Submission received: 11 October 2024 / Revised: 30 October 2024 / Accepted: 30 October 2024 / Published: 1 November 2024

(This article belongs to the Special Issue New Insights into Bioactive Peptides: Design, Synthesis, Structure-Activity Relationship (2nd Edition))

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Abstract

The nonribosomal peptide synthetase (NRPS) is a highly precise molecular assembly machinery for synthesizing structurally diverse peptides, which have broad medicinal applications. Withinthe NRPS, the condensation (C) domain is a core catalytic domain responsible for the formation of amide bonds between individual monomer residues during peptide elongation. This review summarizes various aspects of the C domain, including its structural characteristics, catalytic mechanisms, substrate specificity, substrate gating function, and auxiliary functions. Moreover, through case analyses of the NRPS engineering targeting the C domains, the vast potential of the C domain in the combinatorial biosynthesis of peptide natural product derivatives is demonstrated.

Keywords: nonribosomal peptide synthetase condensation domain; functional diversity of the condensation domains; nonribosomal peptide synthetase engineering targeting the condensation domain

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MDPI and ACS Style

Huang, Z.; Peng, Z.; Zhang, M.; Li, X.; Qiu, X. Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain. Int. J. Mol. Sci. 2024, 25, 11774. https://doi.org/10.3390/ijms252111774

AMA Style

Huang Z, Peng Z, Zhang M, Li X, Qiu X. Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain. International Journal of Molecular Sciences. 2024; 25(21):11774. https://doi.org/10.3390/ijms252111774

Chicago/Turabian Style

Huang, Zhenkuai, Zijing Peng, Mengli Zhang, Xinhai Li, and Xiaoting Qiu. 2024. "Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain" International Journal of Molecular Sciences 25, no. 21: 11774. https://doi.org/10.3390/ijms252111774

APA Style

Huang, Z., Peng, Z., Zhang, M., Li, X., & Qiu, X. (2024). Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain. International Journal of Molecular Sciences, 25(21), 11774. https://doi.org/10.3390/ijms252111774

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Structure, Function and Engineering of the Nonribosomal Peptide Synthetase Condensation Domain

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