The Silencing of GhPIP5K2 and GhPIP5K22 Weakens Abiotic Stress Tolerance in Upland Cotton (Gossypium hirsutum)
Abstract
:1. Introduction
2. Results
2.1. Identification of GhPIP5K Genes and Their Biophysical Properties
2.2. Phylogenetic Analysis of the GhPIP5K Genes
2.3. Chromosome Localization and Duplication Analysis of GhPIP5Ks
2.4. Gene Structure and Conserved Motifs of GhPIP5K Proteins
2.5. Exploration of the Regulatory Elements within the Promoter Regions of GhPIP5K Genes
2.6. Expression Profiling of GhPIP5K Genes in G. hirsutum
2.7. Verification of the Response of GhPIP5Ks to Four Types of Abiotic Stress by qRT‒PCR
2.8. The Silencing of GhPIP5K2 and GhPIP5K22 Compromises the Tolerance of Cotton to Stress
3. Discussion
4. Materials and Methods
4.1. Identification of Members of the PIP5K Gene Family
4.2. Chromosomal Localization and Collinearity Analysis
4.3. Sequence Alignments and Phylogenetic Analysis of the PIP5K Genes
4.4. Analysis of the Gene Structure, Conserved Motifs and Cis-Acting Elements
4.5. Transcriptome Analysis of PIP5K Genes during Growth and Development and under Abiotic Stress Conditions
4.6. Experimental Materials and Treatments
4.7. Extraction of RNA and Quantitative Real-Time Polymerase Chain Reaction (qRT‒PCR) Analysis
4.8. Silencing of GhPIP5K2 and GhPIP5K22 in Cotton
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sequence ID | Gene Name | Nm. of Amino Acids | Molecular Weight | Theoretical pI | Instability Index | Aliphatic Index | Grand Average of Hydropathicity | Subcellular Localization |
---|---|---|---|---|---|---|---|---|
GH_A01G2098 | GhPIP5K1 | 770 | 88,582.97 | 8.59 | 31.89 | 63.94 | −0.71 | nucleus |
GH_A01G2293 | GhPIP5K2 | 825 | 93,092.51 | 8.93 | 43.57 | 76.76 | −0.52 | nucleus |
GH_A02G1290 | GhPIP5K3 | 768 | 88,787.07 | 6.05 | 36.25 | 64.35 | −0.70 | cytosol |
GH_A03G2365 | GhPIP5K4 | 798 | 90,509.07 | 9.06 | 39.79 | 71.03 | −0.56 | nucleus |
GH_A04G0885 | GhPIP5K5 | 815 | 92,004.38 | 8.77 | 47.62 | 78.65 | −0.49 | nucleus |
GH_A04G1543 | GhPIP5K6 | 799 | 90,517.74 | 8.79 | 37.85 | 69.42 | −0.60 | nucleus |
GH_A05G2320 | GhPIP5K7 | 758 | 86,497.71 | 8.50 | 31.74 | 64.79 | −0.63 | nucleus |
GH_A05G2701 | GhPIP5K8 | 729 | 83,795.41 | 8.01 | 34.20 | 63.50 | −0.71 | nucleus |
GH_A05G2841 | GhPIP5K9 | 748 | 85,400.57 | 9.02 | 37.94 | 59.79 | −0.72 | nucleus |
GH_A05G4205 | GhPIP5K10 | 565 | 64,445.81 | 7.57 | 32.24 | 63.27 | −0.59 | cytosol |
GH_A08G1524 | GhPIP5K11 | 416 | 47,724.25 | 8.83 | 53.92 | 76.13 | −0.39 | nucleus |
GH_A10G2027 | GhPIP5K12 | 718 | 81,362.62 | 7.80 | 28.89 | 59.97 | −0.62 | cytosol |
GH_A12G1261 | GhPIP5K13 | 386 | 44,712.66 | 8.47 | 38.77 | 86.58 | −0.23 | cytosol |
GH_A13G0020 | GhPIP5K14 | 779 | 89,410.94 | 8.37 | 35.55 | 66.59 | −0.66 | nucleus |
GH_D01G2192 | GhPIP5K15 | 770 | 88,601.99 | 8.41 | 32.68 | 64.31 | −0.71 | nucleus |
GH_D01G2372 | GhPIP5K16 | 825 | 93,052.47 | 8.93 | 42.74 | 76.06 | −0.53 | nucleus |
GH_D02G2535 | GhPIP5K17 | 800 | 90,634.33 | 9.16 | 39.92 | 69.51 | −0.58 | nucleus |
GH_D03G0675 | GhPIP5K18 | 673 | 77,580.07 | 5.83 | 36.86 | 62.29 | −0.74 | nucleus |
GH_D04G0173 | GhPIP5K19 | 751 | 85,098.08 | 6.72 | 36.26 | 61.49 | −0.65 | nucleus |
GH_D04G1204 | GhPIP5K20 | 821 | 92,894.35 | 8.90 | 49.49 | 76.88 | −0.51 | nucleus |
GH_D04G1886 | GhPIP5K21 | 799 | 90,552.9 | 8.92 | 39.15 | 70.40 | −0.59 | nucleus |
GH_D05G2342 | GhPIP5K22 | 758 | 86,343.47 | 8.41 | 29.17 | 64.27 | −0.64 | nucleus |
GH_D05G2718 | GhPIP5K23 | 729 | 83,625.2 | 8.18 | 34.31 | 62.96 | −0.71 | nucleus |
GH_D05G2857 | GhPIP5K24 | 748 | 85,445.65 | 9.02 | 38.33 | 59.79 | −0.72 | chloroplast |
GH_D08G1541 | GhPIP5K25 | 411 | 46,984.46 | 8.61 | 55.42 | 79.90 | −0.39 | nucleus |
GH_D10G2136 | GhPIP5K26 | 718 | 81,412.64 | 7.52 | 29.62 | 59.83 | −0.63 | cytosol |
GH_D12G1277 | GhPIP5K27 | 384 | 44,353.16 | 8.91 | 35.73 | 82.68 | −0.25 | nucleus |
GH_D13G0016 | GhPIP5K28 | 777 | 89,212.72 | 8.46 | 36.42 | 66.00 | −0.67 | nucleus |
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Ling, P.; Ju, J.; Zhang, X.; Wei, W.; Luo, J.; Li, Y.; Hai, H.; Shang, B.; Cheng, H.; Wang, C.; et al. The Silencing of GhPIP5K2 and GhPIP5K22 Weakens Abiotic Stress Tolerance in Upland Cotton (Gossypium hirsutum). Int. J. Mol. Sci. 2024, 25, 1511. https://doi.org/10.3390/ijms25031511
Ling P, Ju J, Zhang X, Wei W, Luo J, Li Y, Hai H, Shang B, Cheng H, Wang C, et al. The Silencing of GhPIP5K2 and GhPIP5K22 Weakens Abiotic Stress Tolerance in Upland Cotton (Gossypium hirsutum). International Journal of Molecular Sciences. 2024; 25(3):1511. https://doi.org/10.3390/ijms25031511
Chicago/Turabian StyleLing, Pingjie, Jisheng Ju, Xueli Zhang, Wei Wei, Jin Luo, Ying Li, Han Hai, Bowen Shang, Hongbo Cheng, Caixiang Wang, and et al. 2024. "The Silencing of GhPIP5K2 and GhPIP5K22 Weakens Abiotic Stress Tolerance in Upland Cotton (Gossypium hirsutum)" International Journal of Molecular Sciences 25, no. 3: 1511. https://doi.org/10.3390/ijms25031511