The Impact of miR-155-5p on Myotube Differentiation: Elucidating Molecular Targets in Skeletal Muscle Disorders
Abstract
:1. Introduction
2. Results
2.1. Relevance of miR-155 in Different Skeletal Muscle Conditions
2.2. miR-155 Induces a Transcriptional Profile Associated with Morphological Changes
2.3. Biological Processes Enriched in the Transcriptome of miR-155-Treated Myoblasts and Myotubes
2.4. The Transcriptional Overlap between miR-155 Target and DMD Patients
2.5. Direct and Indirect Targets of miR-155-Based Network
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Muscle Differentiation
4.2. Oligonucleotides and Transfection
4.3. Immunostaining
4.4. Total RNA Extraction
4.5. RNA Sequencing
4.6. Expression Pattern Visualization
4.7. Prediction of Direct Target Transcripts of miR-155
4.8. In Silico Prediction of Transcriptional Factors and Kinases
4.9. Enrichment Analysis
4.10. Differential Expression Analysis of Dystrophin-Deficient Patients
4.11. Reconstruction of Molecular Networks and Data Visualization
4.12. Literature Review and Meta-Analysis
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Condition | Clinical or Experimental Study | Ref. |
---|---|---|
Primary muscular disorders | Duchenne muscular dystrophy, facioscapulohumeral muscular dystrophy, limb–girdle muscular dystrophies R1 and R2, Miyoshi myopathy, nemaline myopathy, polymyositis, dermatomyositis, and inclusion body myositis | [22] |
Skeletal muscle differentiation | C2C12 mouse myoblast cells | [27] |
Skeletal muscle differentiation | C2C12 mouse myoblast cells | [30] |
Myogenesis | Primary human skeletal muscle cells (hSkMC) and murine PMI28 cells | [24] |
Skeletal muscle regeneration | Mouse skeletal muscle | [25] |
Insulin-sensitive tissues | Mouse skeletal muscle | [31] |
Skeletal muscle differentiation | C2C12 mouse myoblast cells | [23] |
Skeletal muscle differentiation | Mouse skeletal muscle and C2C12 mouse myoblast cells | [32] |
Sarcopenia and type 2 diabetes mellitus | Human muscle samples | [33] |
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Lopes, L.O.; Cury, S.S.; de Moraes, D.; Oliveira, J.S.; de Oliveira, G.; Cabral-Marques, O.; Fernandez, G.J.; Hirata, M.H.; Wang, D.-Z.; Dal-Pai-Silva, M.; et al. The Impact of miR-155-5p on Myotube Differentiation: Elucidating Molecular Targets in Skeletal Muscle Disorders. Int. J. Mol. Sci. 2024, 25, 1777. https://doi.org/10.3390/ijms25031777
Lopes LO, Cury SS, de Moraes D, Oliveira JS, de Oliveira G, Cabral-Marques O, Fernandez GJ, Hirata MH, Wang D-Z, Dal-Pai-Silva M, et al. The Impact of miR-155-5p on Myotube Differentiation: Elucidating Molecular Targets in Skeletal Muscle Disorders. International Journal of Molecular Sciences. 2024; 25(3):1777. https://doi.org/10.3390/ijms25031777
Chicago/Turabian StyleLopes, Letícia Oliveira, Sarah Santiloni Cury, Diogo de Moraes, Jakeline Santos Oliveira, Grasieli de Oliveira, Otavio Cabral-Marques, Geysson Javier Fernandez, Mario Hiroyuki Hirata, Da-Zhi Wang, Maeli Dal-Pai-Silva, and et al. 2024. "The Impact of miR-155-5p on Myotube Differentiation: Elucidating Molecular Targets in Skeletal Muscle Disorders" International Journal of Molecular Sciences 25, no. 3: 1777. https://doi.org/10.3390/ijms25031777