Cholesterol Content Regulates the Interaction of αA-, αB-, and α-Crystallin with the Model of Human Lens-Lipid Membranes
Abstract
:1. Introduction
2. Results
2.1. Binding of Recombinant Human αAc, αBc, and αABc to Chol/MHLL Membranes
2.2. Mobility near the Headgroup Region of Chol/MHLL Membranes with αAc, αBc, and αABc Binding
2.3. Order near the Headgroup Region of Chol/MHLL Membranes with αAc, αBc, and αABc Binding
2.4. Hydrophobicity near the Headgroup Region of Chol/MHLL Membranes with αAc, αBc, and αABc Binding
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Expression and Purification of Recombinant Human αAc and αBc
4.3. EPR Sample Preparation and Measurements
4.4. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Timsina, R.; Hazen, P.; Trossi-Torres, G.; Khadka, N.K.; Kalkat, N.; Mainali, L. Cholesterol Content Regulates the Interaction of αA-, αB-, and α-Crystallin with the Model of Human Lens-Lipid Membranes. Int. J. Mol. Sci. 2024, 25, 1923. https://doi.org/10.3390/ijms25031923
Timsina R, Hazen P, Trossi-Torres G, Khadka NK, Kalkat N, Mainali L. Cholesterol Content Regulates the Interaction of αA-, αB-, and α-Crystallin with the Model of Human Lens-Lipid Membranes. International Journal of Molecular Sciences. 2024; 25(3):1923. https://doi.org/10.3390/ijms25031923
Chicago/Turabian StyleTimsina, Raju, Preston Hazen, Geraline Trossi-Torres, Nawal K. Khadka, Navdeep Kalkat, and Laxman Mainali. 2024. "Cholesterol Content Regulates the Interaction of αA-, αB-, and α-Crystallin with the Model of Human Lens-Lipid Membranes" International Journal of Molecular Sciences 25, no. 3: 1923. https://doi.org/10.3390/ijms25031923