Normal Proteasome Function Is Needed to Prevent Kidney Graft Injury during Cold Storage Followed by Transplantation
Abstract
:1. Introduction
2. Results
2.1. Characterization of Proteasome Subunits during CS + Tx
2.2. Native Proteasome Composition and Assembly after CS + Tx
2.3. PAC1 and β5 Are Sensitive to ROS during CS + Tx
2.4. Rpt6 Modification during CS + Tx Is Associated with Phosphorylation
2.5. Proteasome Function Is Essential to Maintaining Renal Function following Transplantation
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Rat Surgery and Experimental Groups
4.2.1. Rat Kidney Cold Storage and Transplantation (CS + Tx) Surgery (Donor Kidney)
4.2.2. Autotransplant (ATx) Surgery
4.2.3. Sham Surgery
4.3. Tissue Sample Collection
4.4. In Vitro Cell Model
4.5. Renal Function and Serum Chemistry: iSTAT Measurement
4.6. Proteasome Function
4.7. SDS-PAGE/Native PAGE and Immunoblotting
4.8. Protein Dephosphorylation Assay
4.9. Immunohistochemistry
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bhattarai, D.; Lee, S.-O.; MacMillan-Crow, L.A.; Parajuli, N. Normal Proteasome Function Is Needed to Prevent Kidney Graft Injury during Cold Storage Followed by Transplantation. Int. J. Mol. Sci. 2024, 25, 2147. https://doi.org/10.3390/ijms25042147
Bhattarai D, Lee S-O, MacMillan-Crow LA, Parajuli N. Normal Proteasome Function Is Needed to Prevent Kidney Graft Injury during Cold Storage Followed by Transplantation. International Journal of Molecular Sciences. 2024; 25(4):2147. https://doi.org/10.3390/ijms25042147
Chicago/Turabian StyleBhattarai, Dinesh, Seong-Ok Lee, Lee Ann MacMillan-Crow, and Nirmala Parajuli. 2024. "Normal Proteasome Function Is Needed to Prevent Kidney Graft Injury during Cold Storage Followed by Transplantation" International Journal of Molecular Sciences 25, no. 4: 2147. https://doi.org/10.3390/ijms25042147