Transcriptome Analysis Reveals the Molecular Mechanisms of BR Negative Regulatory Factor StBIN2 Maintaining Tuber Dormancy
Abstract
:1. Introduction
2. Results
2.1. BR Promotes Tuber Sprout by Inhibiting StBIN2
2.2. StBIN2 Maintains Tuber Dormancy
2.3. Analysis and Identification of Differentially Expressed Genes
2.4. Gene Ontology (GO) Analysis
2.5. RT-qPCR of the Selected DEGs
2.6. Physical Interactions of StBIN2 Protein
3. Discussion
4. Material and Method
4.1. Plant Materials and Growth Conditions
4.2. Generation of StBIN2 Transgenic Potato Lines
4.3. Hormone Treatment
4.4. RNA-Seq Analysis
4.5. Validation of RNA-Seq Using RT-qPCR
4.6. Dual-Luciferase Assay
4.7. Protein Interaction Bioinformatics Analysis
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, S.; Cai, C.; Li, L.; Yu, L.; Wang, Q.; Wang, X. Transcriptome Analysis Reveals the Molecular Mechanisms of BR Negative Regulatory Factor StBIN2 Maintaining Tuber Dormancy. Int. J. Mol. Sci. 2024, 25, 2244. https://doi.org/10.3390/ijms25042244
Liu S, Cai C, Li L, Yu L, Wang Q, Wang X. Transcriptome Analysis Reveals the Molecular Mechanisms of BR Negative Regulatory Factor StBIN2 Maintaining Tuber Dormancy. International Journal of Molecular Sciences. 2024; 25(4):2244. https://doi.org/10.3390/ijms25042244
Chicago/Turabian StyleLiu, Shifeng, Chengcheng Cai, Liqin Li, Liping Yu, Qiang Wang, and Xiyao Wang. 2024. "Transcriptome Analysis Reveals the Molecular Mechanisms of BR Negative Regulatory Factor StBIN2 Maintaining Tuber Dormancy" International Journal of Molecular Sciences 25, no. 4: 2244. https://doi.org/10.3390/ijms25042244