Sustained Effectiveness and Safety of Therapeutic miR-10a/b in Alleviating Diabetes and Gastrointestinal Dysmotility without Inducing Cancer or Inflammation in Murine Liver and Colon
Abstract
:1. Introduction
2. Results
2.1. Long-Term Rescue of Diabetes by miR-10a/b Mimics without Inducing Overexpression of miR-10a/b-5p
2.2. Long-Term Rescue of GI Dysmotility by miR-10a/b Mimics in HFHSD-Induced Diabetic Mice without Indication of Risk for Colon Cancer
2.3. Long-Term Treatment with miR-10a/b Mimics in HFHSD-Induced Diabetic Mice Indicates No Risk for Liver Cancer
2.4. Long-Term Treatment with miR-10a/b Mimics in HFHSD-Induced Diabetic Mice Indicates No Risk for Inflammation in Colon and Liver
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Diet
4.3. miR-10a/b Mimic Intervention
4.4. Metabolic Procedure
4.5. Total Gastrointestinal Transit Time Test
4.6. Reverse Transcription Quantitative Polymerase Chain Reaction
4.7. Enzyme-Linked Immunosorbent Assay
4.8. Immunohistochemical and Confocal Microscopy Analysis
4.9. Hematoxylin and Eosin, Oil Red O, and Picro-Sirius Red Staining
4.10. Fluorescence-Activated Cell Sorting
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Singh, R.; Ha, S.E.; Park, H.S.; Debnath, S.; Cho, H.; Baek, G.; Yu, T.Y.; Ro, S. Sustained Effectiveness and Safety of Therapeutic miR-10a/b in Alleviating Diabetes and Gastrointestinal Dysmotility without Inducing Cancer or Inflammation in Murine Liver and Colon. Int. J. Mol. Sci. 2024, 25, 2266. https://doi.org/10.3390/ijms25042266
Singh R, Ha SE, Park HS, Debnath S, Cho H, Baek G, Yu TY, Ro S. Sustained Effectiveness and Safety of Therapeutic miR-10a/b in Alleviating Diabetes and Gastrointestinal Dysmotility without Inducing Cancer or Inflammation in Murine Liver and Colon. International Journal of Molecular Sciences. 2024; 25(4):2266. https://doi.org/10.3390/ijms25042266
Chicago/Turabian StyleSingh, Rajan, Se Eun Ha, Han Sung Park, Sushmita Debnath, Hayeong Cho, Gain Baek, Tae Yang Yu, and Seungil Ro. 2024. "Sustained Effectiveness and Safety of Therapeutic miR-10a/b in Alleviating Diabetes and Gastrointestinal Dysmotility without Inducing Cancer or Inflammation in Murine Liver and Colon" International Journal of Molecular Sciences 25, no. 4: 2266. https://doi.org/10.3390/ijms25042266