Hub Genes and Pathways Related to Lemon (Citrus limon) Leaf Response to Plenodomus tracheiphilus Infection and Influenced by Pseudomonas mediterranea Biocontrol Activity
Abstract
:1. Introduction
2. Results
2.1. Fungus DNA and Disease Index as Affected by P. mediterranea Treatment
2.2. WCGNA Reveals Gene Modules Associated with P. tracheiphilus DNA and Mal Secco Disease Index
2.3. Functional Enrichment Analysis of the Eigengenes in Brown Module
2.4. Functional Analysis of Co-Expression Networks
2.5. Real Time Validation of Hub Genes in Co-Expression Network
3. Discussion
4. Materials and Methods
4.1. Preparation of Pseudomonas mediterranea Cell-Based Bioformulation and of Fungal Pathogen Inoculum
4.2. Transcriptome and Sequencing
4.3. Weighted Correlation Networks Analysis
4.4. Functional Enrichment of Module Genes
4.5. Network Construction
4.6. Validation of Hub Genes by Quantitative Real-Time PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sicilia, A.; Russo, R.; Catara, V.; Lo Piero, A.R. Hub Genes and Pathways Related to Lemon (Citrus limon) Leaf Response to Plenodomus tracheiphilus Infection and Influenced by Pseudomonas mediterranea Biocontrol Activity. Int. J. Mol. Sci. 2024, 25, 2391. https://doi.org/10.3390/ijms25042391
Sicilia A, Russo R, Catara V, Lo Piero AR. Hub Genes and Pathways Related to Lemon (Citrus limon) Leaf Response to Plenodomus tracheiphilus Infection and Influenced by Pseudomonas mediterranea Biocontrol Activity. International Journal of Molecular Sciences. 2024; 25(4):2391. https://doi.org/10.3390/ijms25042391
Chicago/Turabian StyleSicilia, Angelo, Riccardo Russo, Vittoria Catara, and Angela Roberta Lo Piero. 2024. "Hub Genes and Pathways Related to Lemon (Citrus limon) Leaf Response to Plenodomus tracheiphilus Infection and Influenced by Pseudomonas mediterranea Biocontrol Activity" International Journal of Molecular Sciences 25, no. 4: 2391. https://doi.org/10.3390/ijms25042391