Dexamethasone Inhibits White Adipose Tissue Browning
Abstract
:1. Introduction
2. Results
2.1. DXM Treatment Effects in Body Weight, Caloric Intake and Metabolic Parameters
2.2. DXM Inhibited Browning of IAT and RPAT
2.3. DXM Decreased Mitochondrial Content in RPAT and IAT
2.4. Effect of DXM on Gene Expression from Stromal Vascular Fraction (SVF) Cells
2.5. DXM Alters the Expression Pattern of Beige Precursor Cells
2.6. DXM Decreased Thermogenic Capacity on Mature Beige Adipocytes
2.7. DXM Impacts OCR Profile in 3T3-L1 Adipocytes
3. Discussion
4. Materials and Methods
4.1. Animals and Experimental Design
4.2. Metabolic Parameter Measurements
4.3. Liver Lipid Content
4.4. Histological Analysis
4.5. RPAT and IAT Stromal Vascular Fraction Cell Isolation
4.6. RNA Isolation and Quantitative Real-Time PCR (qPCR)
4.7. Western Blotting Assays
4.8. Mitochondrial DNA Quantification
4.9. Gene Expression Analysed in 3T3-L1 Cells
4.10. Oxygen Consumption Rate (OCR) Determination
4.11. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | CTR-RT | DXM-RT | CTR-C | DXM-C | 2-Way ANOVA | ||
---|---|---|---|---|---|---|---|
Cold | DXM | ColdxDXM | |||||
Plasma Glu (g/L) | 1.27 ± 0.05 | 1.25 ± 0.02 | 1.33 ± 0.03 | 1.25 ± 0.04 | ns | ns | ns |
Plasma Tg (g/L) | 1.13 ± 0.10 | 1.72 ± 0.19 | 0.28 ± 0.03 | 0.54 ± 0.09 | p < 0.0001 | p < 0.01 | ns |
Liver Tg (mg/g Liver) | 5.83 ± 0.96 | 5.77 ± 0.84 | 4.22 ± 0.23 | 5.09 ± 0.19 | ns | ns | ns |
Cort (ng/dL) | 4.52 ± 0.89 | 2.62 ± 0.37 | 7.79 ± 2.02 | 4.22 ± 0.93 | p < 0.05 | p < 0.05 | ns |
Primers (5′-3′) | GBAN | bp | |
---|---|---|---|
Actβ | se, AGCCATGTACGTAGCCATCC | NM_031144 | 200 |
as, ACCCTCATAGATGGGCACAG | |||
Arβ3 | se, AGTGGGACTCCTCGTAATGC | NM_013108 | 110 |
as, TTACACAGAGCACGTCCACT | |||
Dio2 | se, CTGGCGCTCTATGACTCGG | NM_031720 | 194 |
as, ACGTGCACCACACTGGAAT | |||
Ebf2 | se, TCTTATCCTACATCCCACACCC | NM_001108383.1 | 113 |
as, TGAGTCTGGTTTCTGTGGTGG | |||
Pdgfrα | se, AGGCTTGGGGCTCACTTTTT | NM_012802 | 153 |
as, AAGAGCTGGCAGACGATGAG | |||
Pgc1α | se, AAAAGCTTGACTGGCGTCAT | NM_031347 | 199 |
as, ACACCACTTCAATCCACCCAG | |||
Ucp-1 | se, CCGAAACTGTACAGCGGTCT | NM_012682 | 155 |
as, GTCATCAAGCCAGCCGAGAT | |||
Zfp423 | se, CCGCGATCGGTGAAAGTTG | NM_001393718.1 | 121 |
as, CACGGCTGGTTTTCCGATCA |
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Giordano, A.P.; Gambaro, S.E.; Alzamendi, A.; Harnichar, A.E.; Rey, M.A.; Ongaro, L.; Spinedi, E.; Zubiría, M.G.; Giovambattista, A. Dexamethasone Inhibits White Adipose Tissue Browning. Int. J. Mol. Sci. 2024, 25, 2714. https://doi.org/10.3390/ijms25052714
Giordano AP, Gambaro SE, Alzamendi A, Harnichar AE, Rey MA, Ongaro L, Spinedi E, Zubiría MG, Giovambattista A. Dexamethasone Inhibits White Adipose Tissue Browning. International Journal of Molecular Sciences. 2024; 25(5):2714. https://doi.org/10.3390/ijms25052714
Chicago/Turabian StyleGiordano, Alejandra Paula, Sabrina Eliana Gambaro, Ana Alzamendi, Alejandro Ezequiel Harnichar, María Amanda Rey, Luisina Ongaro, Eduardo Spinedi, María Guillermina Zubiría, and Andrés Giovambattista. 2024. "Dexamethasone Inhibits White Adipose Tissue Browning" International Journal of Molecular Sciences 25, no. 5: 2714. https://doi.org/10.3390/ijms25052714