A GASA Protein Family Gene, CmGEG, Inhibits Petal Growth in Chrysanthemum
Abstract
:1. Introduction
2. Results
2.1. Cloning and Phylogenetic Analysis of CmGEG
2.2. Subcellular Localization of CmGEG
2.3. The Expression Pattern of CmGEG
2.4. CmGEG Is Involved in Chrysanthemum Petal Elongation
2.5. CmGEG Negatively Regulates Petal Size by Limiting Cell Expansion in Chrysanthemum
3. Discussion
3.1. The GASA Protein CmGEG Localize in the Cytoplasm and Cell Membrane
3.2. CmGEG Inhibited Petal Elongation Regulated by GA
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Cloning and Phylogenetic Analysis of CmGEG
4.3. Ectopic Expression of CmGEG in Arabidopsis
4.4. Subcellular Localization of CmGEG
4.5. Transient Transformation of the Petals
4.6. Measurement of the Petal Size and Cell Size
4.7. RNA Extraction and Quantitative RT-PCR (qRT-PCR)
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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He, Z.; Jiang, R.; Wang, X.; Wang, Y. A GASA Protein Family Gene, CmGEG, Inhibits Petal Growth in Chrysanthemum. Int. J. Mol. Sci. 2024, 25, 3367. https://doi.org/10.3390/ijms25063367
He Z, Jiang R, Wang X, Wang Y. A GASA Protein Family Gene, CmGEG, Inhibits Petal Growth in Chrysanthemum. International Journal of Molecular Sciences. 2024; 25(6):3367. https://doi.org/10.3390/ijms25063367
Chicago/Turabian StyleHe, Ziying, Rui Jiang, Xiaojing Wang, and Yaqin Wang. 2024. "A GASA Protein Family Gene, CmGEG, Inhibits Petal Growth in Chrysanthemum" International Journal of Molecular Sciences 25, no. 6: 3367. https://doi.org/10.3390/ijms25063367