Genome-Wide Identification and Analysis of the Aux/IAA Gene Family in Panax ginseng: Evidence for the Role of PgIAA02 in Lateral Root Development
Round 1
Reviewer 1 Report
Comments and Suggestions for Authorsv This work is very interesting for young researchers to start the functional characterization of genes under abiotic stress.
v I am happy to accept the manuscript, but I have some questions and requested changes. The questions are as follows,
- Protein names should not be given in italics. But gene names must be in italics.
- When mentioning the 'cis-acting elements', the cis should be written in italics. Please revise the corresponding part in the manuscript
-in Figure 3, Cis-element analysis please add the name of the domain.
- Figure 10: Authors are requested to provide additional figures.
- add collinearity analysis.
-The constructs used should also be evaluated and discussed, as the members of other Aux/IAAs could also be affected.
Comments on the Quality of English Language
There are some areas where the quality of the English text could be improved to enhance clarity and flow:
The phrase "It has attracted considerable attention due to its therapeutic potential" could be revised for clarity and conciseness.
The sentence structure could be simplified in some parts for better understanding.
Some sentences could be reworded for readability and coherence.
Here are some examples:
Original: "it has attracted considerable attention due to its therapeutic potential"
Revised: "its therapeutic potential has garnered significant attention"
Original: "Phylogenetic analyses showed that PgIAA was classified into six subgroups and that PgIAA family members in the same group exhibited higher sequence similarity."
Revised: "Phylogenetic analyses classified PgIAA into six subgroups, with members in the same group showing greater sequence similarity."
Original: "Interspecific collinearity analyses suggest that segmental duplications may have been the main driver of PgIAA genes’ evolution and that they underwent purifying selection."
Revised: "Analyses of interspecific collinearity suggest that segmental duplications likely drove the evolution of PgIAA genes, followed by purifying selection."
Original: "RNA-seq data showed that the expression pattern of Aux/IAA genes in Ginseng was tissue specific, and PgIAA02, 36 was specifically highly expressed in lateral, fibrous, and arm roots, suggesting their potential function in root development."
Revised: "RNA-seq data show that the expression pattern of Aux/IAA genes in Ginseng is tissue-specific, and PgIAA02 and PgIAA36 are specifically highly expressed in lateral, fibrous, and arm roots, suggesting their potential function in root development."
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsIn this manuscript, Wang et al. predicted 84 PgIAA genes from the Ginseng genome and performed phylogenetic and structural analyses of the PgIAA gene family. Subsequent experiments explored the subcellular localization of PgIAA02/42/58 proteins and found that overexpression of PgIAA02 inhibited the growth and development of lateral roots. The presentation and data quality of this manuscript are satisfactory, with the authors presenting their findings in a logical manner. However, the authors should pay more attention to the details of their writing. I have the following minor comments:
Line 25: Please provide the full name of Y2H.
Line 26: Please provide the full name of ARF.
Line 51: Please provide the full name of TIR/AFB.
In Figure 1, why is the color of the ends of the chromosomes different from the central part? If the color indicates something, please provide a legend.
Line 127: The word "Arabidopsis" should be italicized.
It is difficult to extract detailed information from Figure 4. Additionally, I think Figure 4B is redundant, as it conveys limited information and is derived from the carrot database, not Ginseng. I suggest deleting Figure 4B and related text.
There appears to be an issue with the layout; Figure 7 is missing, and the figure legends for Figures 7 and 8 are misplaced.
Line 300: The Discussion section should start in a new paragraph.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for AuthorsI have no further comments and believe that the authors have addressed all my questions and the manuscript can now be accepted.
