Microbial Community Changes in Silkworms Suspected of Septicemia and Identification of Serratia sp.
Abstract
:1. Introduction
2. Results
2.1. Morphological Diagnosis of Silkworm Sepsis
2.2. Microbial Diversity in Silkworms with Sepsis
2.3. Isolation and Identification of Bacteria from Silkworms with Sepsis
2.4. Pathogenicity Verification of Isolated Bacteria
3. Discussion
3.1. Characteristics of Silkworms Showing Sepsis Symptoms
3.2. Role of Microbial Community in Inducing Sepsis in Silkworms
3.3. Identification of Causative Bacteria of Sepsis through Verification of Koch’s Postulates
4. Materials and Methods
4.1. Silkworm Sample Preparation
4.2. DNA Extraction and 16S rRNA Amplicon Sequencing
4.3. Microbial Community Analysis
4.4. Isolation and Identification of Microorganisms
4.5. Pathogenicity Verification of Isolated Bacteria
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Park, J.W.; Park, S.K.; Jeong, C.Y.; Kwon, H.G.; Lee, J.H.; Kang, S.K.; Kim, S.-W.; Kim, S.-R. Microbial Community Changes in Silkworms Suspected of Septicemia and Identification of Serratia sp. Int. J. Mol. Sci. 2024, 25, 3957. https://doi.org/10.3390/ijms25073957
Park JW, Park SK, Jeong CY, Kwon HG, Lee JH, Kang SK, Kim S-W, Kim S-R. Microbial Community Changes in Silkworms Suspected of Septicemia and Identification of Serratia sp. International Journal of Molecular Sciences. 2024; 25(7):3957. https://doi.org/10.3390/ijms25073957
Chicago/Turabian StylePark, Jong Woo, Seul Ki Park, Chan Young Jeong, Hyeok Gyu Kwon, Ji Hae Lee, Sang Kuk Kang, Seong-Wan Kim, and Seong-Ryul Kim. 2024. "Microbial Community Changes in Silkworms Suspected of Septicemia and Identification of Serratia sp." International Journal of Molecular Sciences 25, no. 7: 3957. https://doi.org/10.3390/ijms25073957