Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model
Abstract
:1. Introduction
2. Results
2.1. Changes in ERα Gene and Protein Expression in U87 Line Cells Cultured under Different Test Conditions
2.2. Changes in ERα Gene and Protein Expression in Individual GBM Tumoral Areas Obtained from Patients
2.3. Changes in ERβ Gene and Protein Expression in U87 Line Cells Cultured under Different Test Conditions
2.4. Changes in ERβ Gene and Protein Expression in Individual GBM Tumoral Areas Obtained from Patients
3. Discussion
3.1. Estrogen Receptor α in an In Vitro Model
3.2. Estrogen Receptor α in Tumors Taken from Patients
3.3. Estrogen Receptor β in a Model and In Vitro
3.4. Estrogen Receptor β in Tumors Taken from Patients
4. Materials and Methods
4.1. In Vitro Model: Cell Culture
4.2. Model of Tumoral Areas of Patients’ GBM Tumors
4.3. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.4. Enzyme-Linked Immunosorbent Assay (ELISA)
4.5. Immunohistochemistry
4.6. Confocal Microscopy
4.7. Statistical Analysis
5. Conclusions
Research Limitations
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Simińska, D.; Kojder, K.; Jeżewski, D.; Tarnowski, M.; Tomasiak, P.; Piotrowska, K.; Kolasa, A.; Patrycja, K.; Chlubek, D.; Baranowska-Bosiacka, I. Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model. Int. J. Mol. Sci. 2024, 25, 4130. https://doi.org/10.3390/ijms25074130
Simińska D, Kojder K, Jeżewski D, Tarnowski M, Tomasiak P, Piotrowska K, Kolasa A, Patrycja K, Chlubek D, Baranowska-Bosiacka I. Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model. International Journal of Molecular Sciences. 2024; 25(7):4130. https://doi.org/10.3390/ijms25074130
Chicago/Turabian StyleSimińska, Donata, Klaudyna Kojder, Dariusz Jeżewski, Maciej Tarnowski, Patrycja Tomasiak, Katarzyna Piotrowska, Agnieszka Kolasa, Kapczuk Patrycja, Dariusz Chlubek, and Irena Baranowska-Bosiacka. 2024. "Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model" International Journal of Molecular Sciences 25, no. 7: 4130. https://doi.org/10.3390/ijms25074130