Identification of AURKA as a Biomarker Associated with Cuproptosis and Ferroptosis in HNSCC
Abstract
:1. Introduction
2. Results
2.1. Construction of the Prognostic Model in HNSCC
2.2. Evaluation and Validation of the Prognostic Model in HNSCC
2.3. The Relationship between Clinical Characteristics and the Prognostic Model
2.4. Immunological Characteristics of the Prognostic Model
2.5. Functional Enrichment Analysis of the Prognostic Model
2.6. Drug Sensitivity of the Prognostic Model
2.7. Identification of Potential Biomarkers in Constructed Model
2.8. Inhibition of AURKA Suppresses the Proliferation and Migration of HNSCC Cells
3. Discussion
4. Materials and Methods
4.1. Data Acquisition
4.2. Identification of Cuproptosis-Associated Ferroptosis Genes in HNSCC
4.3. Construction of a Predictive Model for HNSCC Based on Cuproptosis-Associated Ferroptosis Genes
4.4. Immune Characteristics and Functional Analysis
4.5. Biological Functional Enrichment Analysis and Drug Sensitivity Analysis
4.6. Cell Culture
4.7. siRNA Transfection
4.8. RT-qPCR
4.9. Cell Counting Kit8 Experiment
4.10. Wound Healing
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jia, X.; Tian, J.; Fu, Y.; Wang, Y.; Yang, Y.; Zhang, M.; Yang, C.; Liu, Y. Identification of AURKA as a Biomarker Associated with Cuproptosis and Ferroptosis in HNSCC. Int. J. Mol. Sci. 2024, 25, 4372. https://doi.org/10.3390/ijms25084372
Jia X, Tian J, Fu Y, Wang Y, Yang Y, Zhang M, Yang C, Liu Y. Identification of AURKA as a Biomarker Associated with Cuproptosis and Ferroptosis in HNSCC. International Journal of Molecular Sciences. 2024; 25(8):4372. https://doi.org/10.3390/ijms25084372
Chicago/Turabian StyleJia, Xiao, Jiao Tian, Yueyue Fu, Yiqi Wang, Yang Yang, Mengzhou Zhang, Cheng Yang, and Yijin Liu. 2024. "Identification of AURKA as a Biomarker Associated with Cuproptosis and Ferroptosis in HNSCC" International Journal of Molecular Sciences 25, no. 8: 4372. https://doi.org/10.3390/ijms25084372