Synthetic Microbial Community Members Interact to Metabolize Caproic Acid to Inhibit Potato Dry Rot Disease
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Identification of Strains That Inhibit Fusarium spp.
2.2. Isolation and Pathogenicity Assay of Pathogens for Dry Rot Disease
2.3. Screening of Synthetic Microbial Communities with Inhibitory Effects on Potato Dry Rot Disease
2.4. Analysis of Active Metabolites in Synthetic Microbial Communities with Inhibitory Activity on Dry Rot Disease
2.5. Validation of Key Metabolites in Synthetic Microbial Communities
2.6. The Biocontrol Mechanism of Caproic Acid
3. Materials and Methods
3.1. Isolation of Microorganisms and Evaluation of Antifungal Activity
3.2. Identification of Microorganisms
3.3. Pathogenicity Assay of Isolated Strains
3.4. Constructing and Screening Synthetic Microbial Communities
3.5. Analysis and Validation of Active Metabolites in Synthetic Microbial Communities
3.6. Detecting the Growth of Bacterial Strains
3.7. Fungal Survival Assay
3.8. Flow Cytometric and Fluorescence Microscopy Assessment of Conidial Viability
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Biocontrol Group | Distribution of Strain Species (Genera) | |
---|---|---|
BM | Monobacteria | Distributed in 10 genera (Bacillus, Paenibacillus, Peribacillus frigoritolerans, Lelliottia amnigena, Serratia proteamaculans, Pseudomonas, Acinetobacter, Actinomycetia bacterium, Paenibacillus amylolyticus, Pantoea agglomerans) |
SynM1 | Bacterial synthesis community (61 strains) | Distributed in 10 genera (Synthesized from all strains in BM) |
SynM2 | Bacterial and fungal synthetic communities (62 strains) | Distributed in 11 genera (SynM1+Penicillium allii) |
SynM3 | Bacillus synthetic community (17 strains) | Distributed in five species (Bacillus sp., Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus pumilus, Bacillus velezensis) |
SynM4 | Lelliottia amnigena synthetic community (11 strains) | Distributed in one species (Lelliottia amnigena) |
SynM5 | Serratia synthetic community (five strains) | Distributed in two species (Serratia plymuthica, Serratia proteamaculans) |
SynM6 | Pseudomonas synthetic community (15 strains) | Distributed in one species (Pseudomonas putida) |
SynM7 | Acinetobacter synthetic community (three strains) | Distributed in one species (Acinetobacter calcoaceticus) |
SynM8 | Bacterial synthesis community (16 strains) | Distributed in nine genera (Bacillus sp., Bacillus amyloliquefaciens, Paenibacillus sp., Bacillus subtilis, Bacillus pumilus, Bacillus velezensis, Peribacillus frigoritolerans, Lelliottia amnigena, Serratia plymuthica, Serratia proteamaculans, Pseudomonas sp., Pseudomonas putida, Acinetobacter sp., Acinetobacter calcoaceticus, Actinomycetia bacterium, Paenibacillus amylolyticus) |
SynM9 | Bacterial and fungal synthetic communities (17 strains) | Distributed in 10 genera (SynM8+Penicillium allii) |
SynM10 | Bacterial synthesis community (six strains) | Distributed in six genera (Bacillus amyloliquefaciens, Serratia proteamaculans, Pseudomonas putida, Acinetobacter calcoaceticus, Actinomycetia bacterium, Paenibacillus amylolyticus) |
SynM11 | Bacterial synthesis community (six strains) | Distributed in six genera (Bacillus subtilis, Serratia proteamaculans, Pseudomonas putida, Acinetobacter calcoaceticus, Actinomycetia bacterium, Paenibacillus amylolyticus) |
SynM12 | Bacterial synthesis community (six strains) | Distributed in six genera (Bacillus pumilus, Serratia proteamaculans, Pseudomonas putida, Acinetobacter calcoaceticus, Actinomycetia bacterium, Paenibacillus amylolyticus) |
SynM13 | Bacterial synthesis community (six strains) | Distributed in six genera (Lelliottia amnigena, Bacillus velezensis, Pseudomonas sp., Serratia proteamaculans, Stenotrophomonas maltophilia, Pantoea agglomerans) |
SynM14 | Bacterial and fungal synthetic communities (seven strains) | Distributed in seven genera (Paenibacillus amylolyticus, Pseudomonas putida, Acinetobacter calcoaceticus, Serratia proteamaculans, Actinomycetia bacterium, Bacillus subtilis, Penicillium allii) |
SynM15 | Bacterial and fungal synthetic communities (seven strains) | Distributed in seven genera (Bacillus pumilus, Pseudomonas putida, Acinetobacter calcoaceticus, Serratia proteamaculans, Actinomycetia bacterium, Bacillus subtilis, Penicillium allii) |
SynM16 | Bacterial and fungal synthetic communities (seven strains) | Distributed in seven genera (Lelliottia amnigena, Bacillus velezensis, Pseudomonas sp., Serratia proteamaculans, Stenotrophomonas maltophilia, Pantoea agglomerans, Penicillium allii) |
SynM17 | Bacterial synthesis community (five strains) | Distributed in five genera (Bacillus velezensis, Pseudomonas sp., Serratia proteamaculans, Stenotrophomonas maltophilia, Pantoea agglomerans) |
SynM18 | Bacterial and fungal synthetic communities (six strains) | Distributed in six genera (Bacillus velezensis, Pseudomonas sp., Serratia proteamaculans, Stenotrophomonas maltophilia, Pantoea agglomerans, Penicillium allii) |
No. | Member Strains in SynM11 | Strains Name |
---|---|---|
1 | B9D10 | Paenibacillus amylolyticus |
2 | B65D14 | Pseudomonas putida |
3 | B9H9 | Acinetobacter calcoaceticus |
4 | B65H4 | Serratia proteamaculans |
5 | B9H11 | Actinomycetia bacterium |
6 | B65D7 | Bacillus subtilis |
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Shi, H.; Li, W.; Chen, H.; Meng, Y.; Wu, H.; Wang, J.; Shen, S. Synthetic Microbial Community Members Interact to Metabolize Caproic Acid to Inhibit Potato Dry Rot Disease. Int. J. Mol. Sci. 2024, 25, 4437. https://doi.org/10.3390/ijms25084437
Shi H, Li W, Chen H, Meng Y, Wu H, Wang J, Shen S. Synthetic Microbial Community Members Interact to Metabolize Caproic Acid to Inhibit Potato Dry Rot Disease. International Journal of Molecular Sciences. 2024; 25(8):4437. https://doi.org/10.3390/ijms25084437
Chicago/Turabian StyleShi, Huiqin, Wei Li, Hongyu Chen, Yao Meng, Huifang Wu, Jian Wang, and Shuo Shen. 2024. "Synthetic Microbial Community Members Interact to Metabolize Caproic Acid to Inhibit Potato Dry Rot Disease" International Journal of Molecular Sciences 25, no. 8: 4437. https://doi.org/10.3390/ijms25084437