Pancreatic β-Cell Identity Change through the Lens of Single-Cell Omics Research
Abstract
:1. Introduction
2. β-Cell Identity
3. β-Cell Identity in Diabetes
4. Endoplasmic Reticulum Stress
5. Oxidative Stress
6. Inflammation
7. Concluding Remarks
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Topic | Species | Method | Identification of β-Cell Heterogeneity Based on: | Ref. |
---|---|---|---|---|
β-cell heterogeneity | Mouse | bulkRNA-seq | Expression of immature β-cell gene panel and lacking UCN3 | [20] |
scRNA-seq | Expression of transcription factor genes Srf, Jun, and Fos | [21] | ||
SCAN-seq ChIP-seq | Heterogeneity in the β-cell epigenome | [22] | ||
Human | scRNA-seq | Presence of cell surface markers ST8S1A1 and CD9 | [23] | |
scRNA-seq | Expression of transcriptional regulators RBP4, FFAR4, ID1, ID2, and ID3 | [25] | ||
scRNA-seq | Expression of oxidative stress-implicated gene FTH1 | [29] | ||
scRNA-seq | Expression of ER stress-inducible genes HERPUD1, HSPA5, and DDIT3 | [30] | ||
snATAC-seq | Heterogeneity in the β-cell epigenome | [31] | ||
scRNA-seq | Expression of novel transcriptional states related to stress | [33] | ||
snRNA-seq scRNA-seq | Expression of INS mRNA levels | [34] | ||
scRNA-seq | Expression of cell surface marker CD63 | [35] | ||
β-cell identity in diabetes | Human | scRNA-seq | Expression of immature β-cell gene panel in islets from T2D donors | [38] |
scRNA-seq | De-repression of juvenile gene sets in β-cells from T2D donors | [42] | ||
scRNA-seq | Reduced expression of INS mRNA levels in β-cells from T2D donors | [25] | ||
bulkRNA-seq proteomics | Expression of β-cell precursor marker ALDOB in islets from T2D donors | [43] | ||
scRNA-seq snATAC-seq | Reduced expression of HNF1A in β-cells from T2D donors | [44] | ||
scRNA-seq | Transcriptional states in β-cells from T1D donors | [45] | ||
scRNA-seq | Transcriptional states in β-cells from T1D and T2D donors | [46] |
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Leenders, F.; de Koning, E.J.P.; Carlotti, F. Pancreatic β-Cell Identity Change through the Lens of Single-Cell Omics Research. Int. J. Mol. Sci. 2024, 25, 4720. https://doi.org/10.3390/ijms25094720
Leenders F, de Koning EJP, Carlotti F. Pancreatic β-Cell Identity Change through the Lens of Single-Cell Omics Research. International Journal of Molecular Sciences. 2024; 25(9):4720. https://doi.org/10.3390/ijms25094720
Chicago/Turabian StyleLeenders, Floris, Eelco J. P. de Koning, and Françoise Carlotti. 2024. "Pancreatic β-Cell Identity Change through the Lens of Single-Cell Omics Research" International Journal of Molecular Sciences 25, no. 9: 4720. https://doi.org/10.3390/ijms25094720