Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses

Liu, Yi; Wang, Cui; Li, Mingxia; Yang, Yunzhou; Wang, Huiying; Chen, Shufang; He, Daqian

doi:10.3390/ijms26083710

Open AccessArticle

Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses

by

Yi Liu

^1,†,

Cui Wang

^1,†

,

Mingxia Li

²,

Yunzhou Yang

¹,

Huiying Wang

¹,

Shufang Chen

^3,* and

Daqian He

^1,*

¹

Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China

²

Academy of Life Sciences and Technology, Tongji University, Shanghai 200092, China

³

NingBo Academy of Agricultural Sciences, Ningbo 315040, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2025, 26(8), 3710; https://doi.org/10.3390/ijms26083710

Submission received: 15 February 2025 / Revised: 3 April 2025 / Accepted: 10 April 2025 / Published: 14 April 2025

(This article belongs to the Special Issue Molecular Regulation of Animal Fat and Muscle Development)

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Abstract

Skeletal muscle satellite cells (SMSCs) are quiescent stem cells located in skeletal muscle tissue and function as the primary reservoir of myogenic progenitors for muscle growth and regeneration. However, the molecular and metabolic mechanisms governing their differentiation in geese remain largely unexplored. This study comprehensively examined the morphological, transcriptional, and metabolic dynamics of goose SMSCs across three critical differentiation stages: the quiescent stage (DD0), the differentiation stage (DD4), and the late differentiation stage (DD6). By integrating transcriptomic and metabolomic analyses, stage-specific molecular signatures and regulatory networks involved in SMSC differentiation were identified. Principal component analysis revealed distinct clustering patterns in gene expression and metabolite profiles across these stages, highlighting dynamic shifts in lipid metabolism and myogenesis. The PPAR signaling pathway emerged as a key regulator, with crucial genes such as PPARG, IGF1, ACSL5, FABP5, and PLIN1 exhibiting differentiation-dependent expression patterns. Notably, PPARG and IGF1 displayed negative correlations with adenosine and L-carnitine levels, suggesting their role in metabolic reprogramming during myotube formation. Additionally, MYOM2 and MYBPC1 exhibited stage-specific regulation and positively correlated with 2,3-dimethoxyphenylamine. This study provides a foundational framework for understanding muscle development and regeneration, offering valuable insights for both agricultural and biomedical research.

Keywords: goose; skeletal muscle satellite cells; transcriptomic; metabolomic; gene expression; differentiation; PPAR signaling pathway

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MDPI and ACS Style

Liu, Y.; Wang, C.; Li, M.; Yang, Y.; Wang, H.; Chen, S.; He, D. Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses. Int. J. Mol. Sci. 2025, 26, 3710. https://doi.org/10.3390/ijms26083710

AMA Style

Liu Y, Wang C, Li M, Yang Y, Wang H, Chen S, He D. Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses. International Journal of Molecular Sciences. 2025; 26(8):3710. https://doi.org/10.3390/ijms26083710

Chicago/Turabian Style

Liu, Yi, Cui Wang, Mingxia Li, Yunzhou Yang, Huiying Wang, Shufang Chen, and Daqian He. 2025. "Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses" International Journal of Molecular Sciences 26, no. 8: 3710. https://doi.org/10.3390/ijms26083710

APA Style

Liu, Y., Wang, C., Li, M., Yang, Y., Wang, H., Chen, S., & He, D. (2025). Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses. International Journal of Molecular Sciences, 26(8), 3710. https://doi.org/10.3390/ijms26083710

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Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses

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