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Volume 26
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10.3390/ijms26093995
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Comprehensive Multi-Omics Analysis of Muscle Tissue Alterations in Male Macrobrachium rosenbergii Induced by Frequent Mating

by
Yunpeng Fan
1,2,
Qiang Gao
1,
Haihua Cheng
1,
Xilian Li
1,
Huwei Yuan
1,
Xue Cai
1,
Lin Tang
1,
Xiudan Yuan
3,
Guangjing Zhang
4 and
Haiqi Zhang
1,*
1
Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China
2
School of Marine Science, Ningbo University, Ningbo 315211, China
3
Hunan Fisheries Science Institute, Changsha 410153, China
4
College of Life Sciences, Hunan Normal University, Changsha 410081, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(9), 3995; https://doi.org/10.3390/ijms26093995
Submission received: 8 March 2025 / Revised: 16 April 2025 / Accepted: 22 April 2025 / Published: 23 April 2025
(This article belongs to the Section Molecular Biology)
Versions Notes

Abstract

During the breeding process of Macrobrachium rosenbergii, a male-to-female ratio of 1:3 or higher is typically adopted, so as a result, the quality of the male broodstock significantly influences the quality of the offspring. We observed that overused males exhibited notable changes in body color, particularly in the tail fan region, which turned orange or red due to the excessive accumulation of astaxanthin in the muscles and exoskeleton. Frequent mating also led to a significant decrease in male body weight, with histological analysis revealing disorganized muscle fiber patterns and increased tissue damage. To investigate the molecular mechanisms underlying these physiological changes, we performed transcriptomic and metabolomic analyses of muscle tissues. A total of 1069 differentially expressed genes (DEGs), 540 differentially expressed proteins (DEPs), and 385 differentially expressed metabolites (DEMs) were identified. Pathway analysis revealed that the DEGs were significantly enriched in pathways related to energy metabolism and degenerative diseases, while the DEMs were notably associated with cancer metabolism, signal transduction, substance transport, energy metabolism, nucleic acid metabolism, neurotransmission, immune response, and metabolic diseases. Proteome analysis showed that proteins and lipids were involved in muscle energy supply. These findings suggest that male M. rosenbergii upregulate energy metabolism in muscles to cope with frequent mating stress, but this adaptation leads to physiological damage. This study provides valuable insights for optimizing male broodstock selection and mating frequency in M. rosenbergii breeding practices.
Keywords: male Macrobrachium rosenbergii; frequent mating; omics; muscle; energy metabolism male Macrobrachium rosenbergii; frequent mating; omics; muscle; energy metabolism

Share and Cite

MDPI and ACS Style

Fan, Y.; Gao, Q.; Cheng, H.; Li, X.; Yuan, H.; Cai, X.; Tang, L.; Yuan, X.; Zhang, G.; Zhang, H. Comprehensive Multi-Omics Analysis of Muscle Tissue Alterations in Male Macrobrachium rosenbergii Induced by Frequent Mating. Int. J. Mol. Sci. 2025, 26, 3995. https://doi.org/10.3390/ijms26093995

AMA Style

Fan Y, Gao Q, Cheng H, Li X, Yuan H, Cai X, Tang L, Yuan X, Zhang G, Zhang H. Comprehensive Multi-Omics Analysis of Muscle Tissue Alterations in Male Macrobrachium rosenbergii Induced by Frequent Mating. International Journal of Molecular Sciences. 2025; 26(9):3995. https://doi.org/10.3390/ijms26093995

Chicago/Turabian Style

Fan, Yunpeng, Qiang Gao, Haihua Cheng, Xilian Li, Huwei Yuan, Xue Cai, Lin Tang, Xiudan Yuan, Guangjing Zhang, and Haiqi Zhang. 2025. "Comprehensive Multi-Omics Analysis of Muscle Tissue Alterations in Male Macrobrachium rosenbergii Induced by Frequent Mating" International Journal of Molecular Sciences 26, no. 9: 3995. https://doi.org/10.3390/ijms26093995

APA Style

Fan, Y., Gao, Q., Cheng, H., Li, X., Yuan, H., Cai, X., Tang, L., Yuan, X., Zhang, G., & Zhang, H. (2025). Comprehensive Multi-Omics Analysis of Muscle Tissue Alterations in Male Macrobrachium rosenbergii Induced by Frequent Mating. International Journal of Molecular Sciences, 26(9), 3995. https://doi.org/10.3390/ijms26093995

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