Next Article in Journal
Liquid Biopsy in Pituitary Neuroendocrine Tumors—Potential Biomarkers for Diagnosis, Prognosis, and Therapy
Previous Article in Journal
The Role of ESS2/DGCR14: Is It an Essential Factor in Splicing and Transcription?
Previous Article in Special Issue
Synthesis of Resorcinol and Chlorophenol from Irradiation of 1,3-Dichlorobenzene in a Water Ice Environment by Low-Energy Electrons
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 26
Issue 9
10.3390/ijms26094057
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Hyperthermal Reactions in DNA Triggered by 1–20 eV Electrons: Absolute Cross Sections for Crosslinks, Strand Breaks, Clustered Damages and Base Modifications

by
Yanfang Dong
1,
Xin Huang
2,
Wenlu Zhang
1,
Yu Shao
2,
Pierre Cloutier
3,
Yi Zheng
2,3 and
Léon Sanche
3,*
1
College of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang 471000, China
2
State Key Laboratory of Photocatalysis on Energy and Environment, Faculty of Chemistry, Fuzhou University, Fuzhou 350116, China
3
Department of Medical Imaging and Radiation Sciences, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(9), 4057; https://doi.org/10.3390/ijms26094057
Submission received: 18 March 2025 / Revised: 11 April 2025 / Accepted: 17 April 2025 / Published: 25 April 2025
(This article belongs to the Special Issue Chemistry Triggered by Low-Energy Particles)
Browse Figures
Versions Notes

Abstract

Absolute cross sections (ACSs) are needed to estimate cellular damage induced by high-energy radiation (HER). Low-energy electrons (LEEs), which are the most numerous secondary particles generated by HER, can trigger hyperthermal reactions in DNA. ACSs for such reactions are essential input parameters to calculate radiobiological effectiveness, particularly in targeted radiotherapy. Using a mathematical model, we generate ACSs from effective damage yields induced by LEE impact on 3197 base-pair plasmid DNA films. Direct or enzyme-revealed conformational damages, quantified by electrophoresis, provide the first complete set of ACSs for inducing crosslinks, double-strand breaks (DSBs), single-strand breaks, base-damage-related crosslinks, non-DSB clustered damages (NDCDs), and isolated base damages. These ACSs are generated across the 1–20 eV range, at one eV intervals. They exhibit a strong energy dependence with maximum values at 10 eV of 3.7 ± 0.8, 3.5 ± 0.6, 45.4 ± 4.1, 2.9 ± 1.1, 5.1 ± 1.4, and 54.0 ± 16.4 × 10−15 cm2, respectively. ACSs for DSBs, NDCDs, and crosslinks clearly indicate that lesions threatening cell function and genetic stability can be generated by a single LEE. At 5 and 10 eV, total damage ACSs are 63% and 80% larger, respectively, than those previously determined for the same plasmids bound to arginine, a constituent of histones protecting DNA.
Keywords: cross sections; low-energy electrons; DNA lesions; radiation damage; Monte Carlo cross sections; low-energy electrons; DNA lesions; radiation damage; Monte Carlo

Share and Cite

MDPI and ACS Style

Dong, Y.; Huang, X.; Zhang, W.; Shao, Y.; Cloutier, P.; Zheng, Y.; Sanche, L. Hyperthermal Reactions in DNA Triggered by 1–20 eV Electrons: Absolute Cross Sections for Crosslinks, Strand Breaks, Clustered Damages and Base Modifications. Int. J. Mol. Sci. 2025, 26, 4057. https://doi.org/10.3390/ijms26094057

AMA Style

Dong Y, Huang X, Zhang W, Shao Y, Cloutier P, Zheng Y, Sanche L. Hyperthermal Reactions in DNA Triggered by 1–20 eV Electrons: Absolute Cross Sections for Crosslinks, Strand Breaks, Clustered Damages and Base Modifications. International Journal of Molecular Sciences. 2025; 26(9):4057. https://doi.org/10.3390/ijms26094057

Chicago/Turabian Style

Dong, Yanfang, Xin Huang, Wenlu Zhang, Yu Shao, Pierre Cloutier, Yi Zheng, and Léon Sanche. 2025. "Hyperthermal Reactions in DNA Triggered by 1–20 eV Electrons: Absolute Cross Sections for Crosslinks, Strand Breaks, Clustered Damages and Base Modifications" International Journal of Molecular Sciences 26, no. 9: 4057. https://doi.org/10.3390/ijms26094057

APA Style

Dong, Y., Huang, X., Zhang, W., Shao, Y., Cloutier, P., Zheng, Y., & Sanche, L. (2025). Hyperthermal Reactions in DNA Triggered by 1–20 eV Electrons: Absolute Cross Sections for Crosslinks, Strand Breaks, Clustered Damages and Base Modifications. International Journal of Molecular Sciences, 26(9), 4057. https://doi.org/10.3390/ijms26094057

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop