Cytotoxicity and Proliferation Studies with Arsenic in Established Human Cell Lines: Keratinocytes, Melanocytes, Dendritic Cells, Dermal Fibroblasts, Microvascular Endothelial Cells, Monocytes and T-Cells
Abstract
:Introduction
Materials and Methods
Chemicals and Cell Culture
Cell Treatment
Cell Treatment for Proliferation
Cell Treatment for Cytotoxicity
Results
Cell Types | Cytotoxicity (LD50) | Peak Proliferation Dose |
Keratinocytes (HaCaT cells) | 9 μg/mL (45.5µM) | 6 μg/mL (30µM) |
Melanocytes (CRL 1675) | 1.5 μg/mL (7.6µM) | 0.19 μg/mL (0.95µM) |
Dendritic cells | 1.5 μg/mL (7.6µM) | 0.19 μg/mL (0.95µM) |
Dermal fibroblasts (CRL1904) | 37 μg/mL (187µM) | 1.5 μg/mL (7.6µM) |
Microvascular endothelial cells (HMEC-1) | 0.48μg/mL (2.4µM) | 0.19 μg/mL (0.95µM) |
Monocytes (TIB202) | 50μg/mL (252.7µM) | 6 μg/mL (30µM) |
Cytotoxicity Assay
Cell Proliferation Assay
Discussion
Acknowledgments
References
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Graham-Evans, B.; Tchounwou, P.B.; Cohly, H.H.P. Cytotoxicity and Proliferation Studies with Arsenic in Established Human Cell Lines: Keratinocytes, Melanocytes, Dendritic Cells, Dermal Fibroblasts, Microvascular Endothelial Cells, Monocytes and T-Cells. Int. J. Mol. Sci. 2003, 4, 13-21. https://doi.org/10.3390/i4010013
Graham-Evans B, Tchounwou PB, Cohly HHP. Cytotoxicity and Proliferation Studies with Arsenic in Established Human Cell Lines: Keratinocytes, Melanocytes, Dendritic Cells, Dermal Fibroblasts, Microvascular Endothelial Cells, Monocytes and T-Cells. International Journal of Molecular Sciences. 2003; 4(1):13-21. https://doi.org/10.3390/i4010013
Chicago/Turabian StyleGraham-Evans, Barbara, Paul B. Tchounwou, and Hari H. P. Cohly. 2003. "Cytotoxicity and Proliferation Studies with Arsenic in Established Human Cell Lines: Keratinocytes, Melanocytes, Dendritic Cells, Dermal Fibroblasts, Microvascular Endothelial Cells, Monocytes and T-Cells" International Journal of Molecular Sciences 4, no. 1: 13-21. https://doi.org/10.3390/i4010013