A PCR-Based Retrospective Study for Beak and Feather Disease Virus (BFDV) in Five Wild Populations of Parrots from Australia, Argentina and New Zealand
Abstract
:1. Introduction
2. Methods
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Altizer, S.; Harvell, D.; Friedle, E. Rapid evolutionary dynamics and disease threats to biodiversity. Trends Ecol. Evol. 2003, 18, 589–596. [Google Scholar] [CrossRef]
- Steinmetz, H.W.; Bakonyi, T.; Weissenböck, H.; Hatt, J.-M.; Eulenberger, U.; Robert, N.; Hoop, R.; Nowotny, N. Emergence and establishment of Usutu virus infection in wild and captive avian species in and around Zurich, Switzerland—Genomic and pathologic comparison to other central European outbreaks. Vet. Microbiol. 2011, 148, 207–212. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kleyheeg, E.; Slaterus, R.; Bodewes, R.; Rijks, J.; Spierenburg, M.A.H.; Beerens, N.; Kelder, L.; Poen, M.; Stegeman, J.; Fouchier, R.A.M.; et al. Deaths among wild birds during highly pathogenic Avian Influenza A(H5N8) Virus Outbreak, The Netherlands. Emerg. Infect. Dis. J. 2017, 23, 2050. [Google Scholar] [CrossRef] [PubMed]
- Krone, O.; Globig, A.; Ulrich, R.; Harder, T.; Schinköthe, J.; Herrmann, C.; Gerst, S.; Conraths, F.J.; Beer, M. White-Tailed Sea Eagle (Haliaeetus albicilla) die-off due to infection with highly pathogenic Avian Influenza Virus, Subtype H5N8, in Germany. Viruses 2018, 10, 478. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jackson, H.; Strubbe, D.; Tollington, S.; Prys-Jones, R.; Matthysen, E.; Groombridge, J.J. Ancestral origins and invasion pathways in a globally invasive bird correlate with climate and influences from bird trade. Mol. Ecol. 2015, 24, 4269–4285. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fogell, D.J.; Martin, R.O.; Bunbury, N.; Lawson, B.; Sells, J.; McKeand, A.M.; Tatayah, V.; Trung, C.T.; Groombridge, J.J. Trade and conservation implications of new beak and feather disease virus detection in native and introduced parrots. Conserv. Biol. 2018, 32, 1325–1335. [Google Scholar] [CrossRef] [Green Version]
- Ortiz-Catedral, L.; Brunton, D.; Stidworthy, M.F.; Elsheikha, H.M.; Pennycott, T.; Schulze, C.; Braun, M.; Wink, M.; Gerlach, H.; Pendl, H.; et al. Haemoproteus minutus is highly virulent for Australasian and South American parrots. Parasites Vectors 2019, 12, 40. [Google Scholar] [CrossRef] [PubMed]
- Fogell, D.J.; Martin, R.O.; Groombridge, J.J. Beak and feather disease virus in wild and captive parrots: An analysis of geographic and taxonomic distribution and methodological trends. Arch. Virol. 2016, 161, 2059–2074. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jayasinghe, M.; Midwinter, A.; Roe, W.; Vallee, E.; Bolwell, C.; Gartrell, B. Seabirds as possible reservoirs of Erysipelothrix rhusiopathiae on islands used for conservation translocations in New Zealand. J. Wildl. Dis. 2021, 57, 534–542. [Google Scholar] [CrossRef]
- Olah, G.; Butchart, S.H.M.; Symes, A.; Guzmán, I.M.; Cunningham, R.; Brightsmith, D.J.; Heinsohn, R. Ecological and socio-economic factors affecting extinction risk in parrots. Biodivers. Conserv. 2016, 25, 205–223. [Google Scholar] [CrossRef]
- Berkunsky, I.; Quillfeldt, P.; Brightsmith, D.J.; Abbud, M.C.; Aguilar, J.M.R.E.; Alemán-Zelaya, U.; Aramburú, R.M.; Arce Arias, A.; Balas McNab, R.; Balsby, T.J.S.; et al. Current threats faced by Neotropical parrot populations. Biol. Conserv. 2017, 214, 278–287. [Google Scholar] [CrossRef] [Green Version]
- Ortiz-Catedral, L.; Nias, R.; Fitzsimons, J.; Vine, S.; Christian, M. Back from the brink–again: The decline and recovery of the Norfolk Island green parrot. In Recovering Australian Threatened Species: A Book of Hope; Garnett, S., Latch, P., Lindenmayer, D., Woinarski, J., Eds.; CSIRO Publishing: Clayton South, Australia, 2018. [Google Scholar]
- Snyder, N.; McGowan, P.; Gilardi, J.; Grajal, A. Parrots. Status Survey and Conservation Action Plan 2000–2004; IUCN: Gland, Switzerland; Cambridge, UK, 2000. [Google Scholar]
- Regnard, G.L.; Boyes, R.S.; Martin, R.; Hitzeroth, I.I.; Rybicki, E.P. Beak and feather disease viruses circulating in Cape parrots (Poicepahlus robustus) in South Africa. Arch. Virol. 2015, 160, 47–54. [Google Scholar] [CrossRef] [PubMed]
- Das, S.; Smith, K.; Sarker, S.; Peters, A.; Adriaanse, K.; Eden, P.; Ghorashi, S.A.; Forwood, J.K.; Raidal, S.R. Repeat spillover of beak and feather disease virus into an endangered parrot highlights the risk associated with endemic pathogen loss in endangered species. J. Wildl. Dis. 2020, 56, 896–906. [Google Scholar] [CrossRef] [PubMed]
- Wilson, M.H.; Kepler, C.B.; Snyder, N.F.R.; Derrickson, S.R.; Dein, F.J.; Wiley, J.W.; Wunderle, J.M.; Lugo, A.E.; Graham, D.L.; Toone, W.D. Puerto Rican Parrots and potential limitations of the metapopulation approach to species conservation. Conserv. Biol. 1994, 8, 114–123. [Google Scholar] [CrossRef] [Green Version]
- Gartrell, B.D.; Alley, M.R.; Mack, H.; Donald, J.; McInnes, K.; Jansen, P. Erysipelas in the critically endangered kakapo (Strigops habroptilus). Avian Pathol. 2005, 34, 383–387. [Google Scholar] [CrossRef] [PubMed]
- Julian, L.; Piasecki, T.; Chrząstek, K.; Walters, M.; Muhire, B.; Harkins, G.W.; Martin, D.P.; Varsani, A. Extensive recombination detected among beak and feather disease virus isolates from breeding facilities in Poland. J. Gen. Virol. 2013, 94, 1086–1095. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Piasecki, T.; Harkins, G.W.; Chrząstek, K.; Julian, L.; Martin, D.P.; Varsani, A. Avihepadnavirus diversity in parrots is comparable to that found amongst all other avian species. Virology 2013, 438, 98–105. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Raidal, S.R.; Mcelnea, C.L.; Cross, G.M. Seroprevalence of psittacine beak and feather disease in wild psittacine birds in New-South-Wales. Aust. Vet. J. 1993, 70, 137–139. [Google Scholar] [CrossRef]
- Gilardi, K.V.; Lowenstine, L.J.; Gilardi, J.D.; Munn, C.A. A survey for selected viral, chlamydial, and parasitic diseases in wild dusky-headed parakeets (Aratinga weddellii) and tui parakeets (Brotogeris sanctithomae) in Peru. J. Wildl. Dis. 1995, 31, 523–528. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Deem, S.L.; Noss, A.J.; Cuellar, R.L.; Karesh, W.B. Health evaluation of free-ranging and captive blue-fronted Amazon parrots (Amazona aestiva) in the Gran Chaco, Bolivia. J. Zoo Wildl. Med. 2005, 36, 598–605. [Google Scholar] [CrossRef]
- Ha, H.J.; Anderson, I.L.; Alley, M.R.; Springett, B.P.; Gartrell, B.D. The prevalence of beak and feather disease virus infection in wild populations of parrots and cockatoos in New Zealand. N. Z. Vet. J. 2007, 55, 235–238. [Google Scholar] [CrossRef] [PubMed]
- Ortiz-Catedral, L.; Ismar, S.M.H.; Baird, K.; Ewen, J.G.; Hauber, M.E.; Brunton, D.H. No evidence of Campylobacter, Salmonella and Yersinia in free-living populations of the red-crowned parakeet (Cyanoramphus novaezelandiae). N. Z. J. Zool. 2009, 36, 379–383. [Google Scholar] [CrossRef]
- Ortiz-Catedral, L.; McInnes, K.; Hauber, M.E.; Brunton, D.H. First report of beak and feather disease virus (BFDV) in wild Red-fronted Parakeets (Cyanoramphus novaezelandiae) in New Zealand. Emu 2009, 109, 244–247. [Google Scholar] [CrossRef]
- Martens, J.M.; Stokes, H.S.; Berg, M.L.; Walder, K.; Bennett, A.T.D. Seasonal fluctuation of beak and feather disease virus (BFDV) infection in wild Crimson Rosellas (Platycercus elegans). Sci. Rep. 2020, 10, e7894. [Google Scholar] [CrossRef] [PubMed]
- Martens, J.M.; Stokes, H.S.; Berg, M.L.; Walder, K.; Raidal, S.R.; Magrath, M.J.; Bennett, A.T. A non-invasive method to assess environmental contamination with avian pathogens: Beak and feather disease virus (BFDV) detection in nest boxes. PeerJ 2020, 8, e9211. [Google Scholar] [CrossRef]
- Martens, J.M.; Stokes, H.S.; Berg, M.L.; Walder, K.; Raidal, S.R.; Magrath, M.J.L.; Bennett, A.T.D. Beak and feather disease virus (BFDV) prevalence, load and excretion in seven species of wild caught common Australian parrots. PLoS ONE 2020, 15, e0235406. [Google Scholar] [CrossRef]
- Vaz, F.F.; Sipinski, E.A.B.; Seixas, G.H.F.; Prestes, N.P.; Martinez, J.; Raso, T.F. Molecular Survey of Pathogens in Wild Amazon Parrot Nestlings: Implications for Conservation. Diversity 2021, 13, 272. [Google Scholar] [CrossRef]
- Breitbart, M.; Delwart, E.; Rosario, K.; Segalés, J.; Varsani, A.; ICTV Report Consortium. ICTV Virus Taxonomy Profile: Circoviridae. J. Gen. Virol. 2017, 98, 1997–1998. [Google Scholar] [CrossRef]
- Rosario, K.; Breitbart, M.; Harrach, B.; Segalés, J.; Delwart, E.; Biagini, P.; Varsani, A. Revisiting the taxonomy of the family Circoviridae: Establishment of the genus Cyclovirus and removal of the genus Gyrovirus. Arch. Virol. 2017, 162, 1447–1463. [Google Scholar] [CrossRef] [Green Version]
- Raidal, S.R.; Peters, A. Psittacine beak and feather disease: Ecology and implications for conservation. Emu 2018, 118, 80–93. [Google Scholar] [CrossRef]
- Todd, D. Circoviruses: Immunosuppressive threats to avian species: A review. Avian Pathol. 2000, 29, 373–394. [Google Scholar] [CrossRef] [PubMed]
- Ortiz-Catedral, L. No T-cell-mediated immune response detected in a red-fronted parakeet (Cyanoramphus novaezelandiae) infected with the Beak and Feather Disease Virus (BFDV). Notornis 2010, 57, 81–84. [Google Scholar]
- Ritchie, B.W.; Niagro, F.D.; Lukert, P.D.; Latimer, K.S.; Steffens III, W.L.; Pritchard, N. A review of psittacine beak and feather disease: Characteristics of the PBFD virus. J. Assoc. Avian Vet. 1989, 3, 143–149. [Google Scholar] [CrossRef]
- Kock, N.; Hangartner, P.; Lucke, V. Variation in clinical disease and species susceptibility to psittacine beak and feather disease in Zimbabwean lovebirds. J. Vet. Res. 1993, 60, 159–161. [Google Scholar]
- Malham, J.; Kovac, E.; Reuleaux, A.; Linnebjerg, J.; Tollington, S.; Raisin, C.; Marsh, P.; McPherson, S. Results of Screening Echo and Ringneck Parakeets for Psittacine Beak and Feather Disease in Mauritius March 2008; Mauritian Wildlife Foundation: Vacoas-Phoenix, Mauritius; National Parks and Conservation Service of Mauritius: Reduit, Mauritius; International Zoo Veterinary Group: Keighley, UK; Durrel Wildlife Conservation Trust: Jersey, Channel Islands; IBL Aviation, Shipping and Other Services: Port Louis, Mauritius; Chester Zoo: Chester, UK; The World Parrot Trust: Hayle, UK, 2008. [Google Scholar]
- Peters, A.; Patterson, E.I.; Baker, B.G.B.; Holdsworth, M.; Sarker, S.; Ghorashi, S.A.; Raidal, S.R. Evidence of psittacine beak and feather disease virus spillover into wild critically endangered orange-bellied parrots (Neophema chrysogaster). J. Wildl. Dis. 2014, 50, 288–296. [Google Scholar] [CrossRef] [PubMed]
- Olah, G.; Smith, B.T.; Joseph, L.; Banks, S.C.; Heinsohn, R. Advancing Genetic Methods in the Study of Parrot Biology and Conservation. Diversity 2021, 13, 521. [Google Scholar] [CrossRef]
- Amery-Gale, J.; Marenda, M.; Owens, J.; Eden, P.A.; Browning, G.; Devlin, J. A high prevalence of beak and feather disease virus in non-psittacine Australian birds. J. Med. Microbiol. 2017, 66, 1005–1013. [Google Scholar] [CrossRef]
- Varsani, A.; Regnard, G.L.; Bragg, R.; Hitzeroth, I.I.; Rybicki, E.P. Global genetic diversity and geographical and host-species distribution of beak and feather disease virus isolates. J. Gen. Virol. 2011, 92, 752–767. [Google Scholar] [CrossRef] [Green Version]
- Fogell, D.J.; Tollington, S.; Tatayah, V.; Henshaw, S.; Naujeer, H.; Jones, C.; Raisin, C.; Greenwood, A.; Groombridge, J.J. Evolution of Beak and Feather Disease Virus across three decades of conservation intervention for population recovery of the Mauritius parakeet. Diversity 2021, 13, 584. [Google Scholar] [CrossRef]
- Ortiz-Catedral, L.; Kearvell, J.; Brunton, D.H. Re-introduction of captive-bred Malherbe’s parakeet to Maud Island, Marlborough Sounds, New Zealand. In Global Re-Introduction Perspectives: Additional Case-Studies from around the Globe; Soorae, P.S., Ed.; IUCN/SSC Re-Introduction Specialist Group: Abu Dhabi, United Arab Emirates, 2010; pp. 151–154. [Google Scholar]
- Varsani, A.; Villiers, G.; Regnard, G.; Bragg, R.; Kondiah, K.; Hitzeroth, I.; Rybicki, E. A unique isolate of beak and feather disease virus isolated from budgerigars (Melopsittacus undulatus) in South Africa. Arch. Virol. 2010, 155, 435–439. [Google Scholar] [CrossRef]
- Julian, L.; Lorenzo, A.; Chenuet, J.-P.; Bonzon, M.; Marchal, C.; Vignon, L.; Collings, D.A.; Walters, M.; Jackson, B.; Varsani, A. Evidence of multiple introductions of beak and feather disease virus into the Pacific islands of Nouvelle-Caledonie (New Caledonia). J. Gen. Virol. 2012, 93, 2466–2472. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Massaro, M.; Ortiz-Catedral, L.; Julian, L.; Galbraith, J.A.; Kurenbach, B.; Kearvell, J.; Kemp, J.; van Hal, J.; Elkington, S.; Taylor, G.; et al. Molecular characterisation of beak and feather disease virus (BFDV) in New Zealand and its implications for managing an infectious disease. Arch. Virol. 2012, 157, 1651–1663. [Google Scholar] [CrossRef] [PubMed]
- Jackson, B.; Harvey, C.; Galbraith, J.; Robertson, M.; Warren, K.; Holyoake, C.; Julian, L.; Varsani, A. Clinical beak and feather disease virus infection in wild juvenile eastern rosellas of New Zealand; biosecurity implications for wildlife care facilities. N. Z. Vet. J. 2014, 62, 297–301. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Eastwood, J.R.; Berg, M.L.; Spolding, B.; Buchanan, K.L.; Bennett, A.T.D.; Walder, K. Prevalence of beak and feather disease virus in wild Platycercus elegans: Comparison of three tissue types using a probe-based real-time qPCR test. Aust. J. Zool. 2015, 63, 1–8. [Google Scholar] [CrossRef] [Green Version]
- Raidal, S.R.; Sarker, S.; Peters, A. Review of psittacine beak and feather disease and its effect on Australian endangered species. Aust. Vet. J. 2015, 93, 466–470. [Google Scholar] [CrossRef]
- Rahaus, M.; Desloges, N.; Probst, S.; Loebbert, B.; Lantermann, W.; Wolff, M. Detection of beak and feather disease virus DNA in embryonated eggs of psittacine birds. Vet. Med. 2008, 53, 53–58. [Google Scholar] [CrossRef] [Green Version]
- Legge, S.; Heinsohn, R.; Garnett, S. Availability of nest hollows and breeding population size of eclectus parrots, Eclectus roratus, on Cape York Peninsula, Australia. Wildlife Res. 2004, 31, 149–161. [Google Scholar] [CrossRef]
- Krebs, E.A. Breeding biology of crimson rosellas (Platycercus elegans) on Black Mountain, Australian Capital Territory. Aust. J. Zool. 1998, 46, 119–136. [Google Scholar] [CrossRef]
- Bliss, T. Forbes’ Parakeet (Cyanoramphus forbesi) Report on Field Work during the 2012–2015 Breeding Seasons and Analysis of Monitoring Results 1999 to 2015; Department of Conservation: Chatham Islands, New Zealand, 2016.
- Masello, J.F.; Pagnossin, M.L.; Sommer, C.; Quillfeldt, P. Population size, provisioning frequency, flock size and foraging range at the largest known colony of Psittaciformes: The Burrowing Parrots of the north-eastern Patagonian coastal cliffs. Emu 2006, 106, 69–79. [Google Scholar] [CrossRef]
- Bucher, E.H.; Martin, L.F.; Martella, M.B.; Navarro, J.L. Social behaviour and population dynamics of the Monk Parakeet. Proc. Int. Ornithol. Congr. 1991, 20, 681–689. [Google Scholar]
- Heinsohn, R.; Legge, S.; Endler, J.A. Extreme Reversed Sexual Dichromatism in a Bird Without Sex Role Reversal. Science 2005, 309, 617–619. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Heinsohn, R.; Ebert, D.; Legge, S.; Peakall, R. Genetic evidence for cooperative polyandry in reverse dichromatic Eclectus parrots. Anim. Behav. 2007, 74, 1047–1054. [Google Scholar] [CrossRef]
- Arctander, P. Comparative studies of avian DNA by restriction fragment length polymorphism analysis: Convenient procedures based on blood samples from live birds. J. Ornithol. 1988, 129, 205–216. [Google Scholar] [CrossRef]
- Seutin, G.; White, B.N.; Boag, P.T. Preservation of avian blood and tissue samples for DNA analyses. Can. J. Zool. 2014, 69, 82–90. [Google Scholar] [CrossRef]
- Jackson, B.; Varsani, A.; Holyoake, C.; Jakob-Hoff, R.; Robertson, I.; McInnes, K.; Empson, R.; Gray, R.; Nakagawa, K.; Warren, K. Emerging infectious disease or evidence of endemicity? A multi-season study of beak and feather disease virus in wild red-crowned parakeets (Cyanoramphus novaezelandiae). Arch. Virol. 2015, 160, 2283–2292. [Google Scholar] [CrossRef] [PubMed]
- Ritchie, P.A.; Anderson, I.L.; Lambert, D.M. Evidence for specificity of psittacine beak and feather disease viruses among avian hosts. Virology 2003, 306, 109–115. [Google Scholar] [CrossRef]
- Ortiz-Catedral, L.; Kurenbach, B.; Massaro, M.; McInnes, K.; Brunton, D.; Hauber, M.; Martin, D.; Varsani, A. A new isolate of beak and feather disease virus from endemic wild red-fronted parakeets (Cyanoramphus novaezelandiae) in New Zealand. Arch. Virol. 2010, 155, 613–620. [Google Scholar] [CrossRef]
- Leendertz, F.H.; Pauli, G.; Maetz-Rensing, K.; Boardman, W.; Nunn, C.; Ellerbrok, H.; Jensen, S.A.; Junglen, S.; Christophe, B. Pathogens as drivers of population declines: The importance of systematic monitoring in great apes and other threatened mammals. Biol. Conserv. 2006, 131, 325–337. [Google Scholar] [CrossRef]
- Khalesi, B.; Bonne, N.; Stewart, M.; Sharp, M.; Raidal, S. A comparison of haemagglutination, haemagglutination inhibition and PCR for the detection of psittacine beak and feather disease virus infection and a comparison of isolates obtained from loriids. J. Gen. Virol. 2005, 86, 3039–3046. [Google Scholar] [CrossRef]
- Sariya, L.; Prompiram, P.; Khocharin, W.; Tangsugjai, S.; Phonarknguen, R.; Ratanakorn, P.; Chaichoun, K. Genetic analysis of beak and feather disease virus isolated from captive psittacine birds in Thailand. Southeast Asian J. Trop. Med. Public Health 2011, 42, 851–858. [Google Scholar] [PubMed]
- Hermes, N.; Evans, O.; Evans, B. Norfolk Island birds: A review. Notornis 1986, 33, 141–149. [Google Scholar]
- Peachey, M. Psittacine beak and feather viral disease in parrots in the ACT. Canberra Bird Notes 2013, 38, 106–118. [Google Scholar]
- Morinha, F.; Carrete, M.; Tella, J.L.; Blanco, G. High prevalence of novel beak and feather disease virus in sympatric invasive parakeets introduced to Spain from Asia and South America. Diversity 2020, 12, 192. [Google Scholar] [CrossRef]
- Heath, L.; Martin, D.P.; Warburton, L.; Perrin, M.; Horsfield, W.; Kingsley, C.; Rybicki, E.P.; Williamson, A.-L. Evidence of unique genotypes of psittacine beak and feather disease in southern Africa. J. Virol. 2004, 78, 9277–9284. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Raue, R.; Johne, R.; Crosta, L.; Burkle, M.; Gerlach, H.; Muller, H. Nucleotide sequence analysis of a C1 gene fragment of psittacine beak and feather disease virus amplified by real-time polymerase chain reaction indicates a possible existence of genotypes. Avian Pathol. 2004, 33, 41–50. [Google Scholar] [CrossRef] [PubMed]
Species | Estimation of Population Size | Reference for Population Size | Year of Sample Collection | Blood Samples (n) | Total | |
---|---|---|---|---|---|---|
Adult | Nestling | |||||
Psittaculini | ||||||
Eclectus roratus | 3000 | [51] | 1997–2007 | 24 | 291 | 315 |
Platycercini | ||||||
Platycercuselegans | 550 | [52] | 1993–1995 | 17 | 52 | 69 |
Cyanoramphus forbesi | 1000 | [53] | 2014 | 95 | − | 95 |
Arini | ||||||
Cyanoliseus patagonus | 75,000 | [54] | December 1998, December 1999 | 49t | 55 | 104 |
Myopsitta monachus | 500 | [55] and E.H.B. unpubl. data | December 2000 | 29 | − | 29 |
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Ortiz-Catedral, L.; Wallace, C.J.; Heinsohn, R.; Krebs, E.A.; Langmore, N.E.; Vukelic, D.; Bucher, E.H.; Varsani, A.; Masello, J.F. A PCR-Based Retrospective Study for Beak and Feather Disease Virus (BFDV) in Five Wild Populations of Parrots from Australia, Argentina and New Zealand. Diversity 2022, 14, 148. https://doi.org/10.3390/d14020148
Ortiz-Catedral L, Wallace CJ, Heinsohn R, Krebs EA, Langmore NE, Vukelic D, Bucher EH, Varsani A, Masello JF. A PCR-Based Retrospective Study for Beak and Feather Disease Virus (BFDV) in Five Wild Populations of Parrots from Australia, Argentina and New Zealand. Diversity. 2022; 14(2):148. https://doi.org/10.3390/d14020148
Chicago/Turabian StyleOrtiz-Catedral, Luis, Connor J. Wallace, Robert Heinsohn, Elizabeth A. Krebs, Naomi E. Langmore, Dusan Vukelic, Enrique H. Bucher, Arvind Varsani, and Juan F. Masello. 2022. "A PCR-Based Retrospective Study for Beak and Feather Disease Virus (BFDV) in Five Wild Populations of Parrots from Australia, Argentina and New Zealand" Diversity 14, no. 2: 148. https://doi.org/10.3390/d14020148