Journal Description
Diversity
Diversity
is a peer-reviewed, open access journal on the science of biodiversity from molecules, genes, populations, and species, to ecosystems and is published monthly online by MDPI.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubAg, GEOBASE, CAPlus / SciFinder, and other databases.
- Journal Rank: JCR - Q2 (Biodiversity Conservation) / CiteScore - Q2 (Agricultural and Biological Sciences (miscellaneous))
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 18.3 days after submission; acceptance to publication is undertaken in 4.4 days (median values for papers published in this journal in the second half of 2024).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
- Diversity is a companion journal of Fossil Studies.
Impact Factor:
2.1 (2023);
5-Year Impact Factor:
2.3 (2023)
Latest Articles
Assessing Local Distribution of Alien Ponto-Caspian Mysids in Lithuanian Waters, the Baltic Sea Basin: Do Sampling Method and Time Matter?
Diversity 2025, 17(5), 307; https://doi.org/10.3390/d17050307 - 23 Apr 2025
Abstract
Alien Ponto-Caspian mysids Paramysis lacustris, Limnomysis benedeni and Hemimysis anomala were introduced into Lithuanian waters from Ukraine’s water reservoirs in the early 1960s. Their expansion from the primary introduction site in the Kaunas Reservoir, located on the Nemunas River, proceeded through secondary
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Alien Ponto-Caspian mysids Paramysis lacustris, Limnomysis benedeni and Hemimysis anomala were introduced into Lithuanian waters from Ukraine’s water reservoirs in the early 1960s. Their expansion from the primary introduction site in the Kaunas Reservoir, located on the Nemunas River, proceeded through secondary introductions and natural dispersal. The two species, P. lacustris and L. benedeni, are currently quite widespread in Lithuanian waters, whereas H. anomala has been observed exclusively in the Kaunas Reservoir until recently. Here, we present data from the most recent comprehensive survey of Ponto Caspian mysids and analyze the impact of sampling method on the likelihood of species detection. The results clearly indicate that the detection of larger-sized, more mobile species with good swimming abilities, such as P. lacustris, requires, in addition to conventional macroinvertebrate sampling, the use of devices designed to capture active nektobenthic animals. For this purpose, an epibenthic dredge or sledge is recommended. In contrast, the detection probability of the smaller-sized L. benedeni was not affected by the sampling method. The recent detection of the bloody-red mysid H. anomala near the Nemunas Delta suggests it may now be well-established in the area. However, due to its nocturnal lifestyle, the effective detection of this mysid requires dusk or nighttime sampling using equipment appropriate for capturing nektobenthic fauna.
Full article
(This article belongs to the Section Freshwater Biodiversity)
Open AccessArticle
Attitudes Toward Managing a Fish-Eating Predator, the Great Cormorant, in a Coastal Environment
by
Vasilios Liordos, Vasileios J. Kontsiotis, Georgios Karras, Christina Kyriakidou and Georgios Karris
Diversity 2025, 17(5), 306; https://doi.org/10.3390/d17050306 - 23 Apr 2025
Abstract
The population of the continental race of the great cormorant (Phalacrocorax carbo sinensis) has significantly increased over the last few decades due to legal protection. This rise has led to intense conflicts with fishing interests because of the bird’s fish-eating habits.
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The population of the continental race of the great cormorant (Phalacrocorax carbo sinensis) has significantly increased over the last few decades due to legal protection. This rise has led to intense conflicts with fishing interests because of the bird’s fish-eating habits. Effective conflict management requires an understanding of public attitudes. We collected data through interviews with 260 residents (50 fishers and 210 members of the general public) to examine the endorsement and prioritization of strategies to manage great cormorants in a fishery in northern Greece. First, we asked respondents to state their endorsement of implementing each of six management strategies, with possible responses being “endorsed” or “not endorsed.” Then, we asked them to select the one they would prioritize among the six strategies for implementation. The most endorsed management strategy among all residents was using nets to cover fish wintering channels (85.7%), followed by compensation for damage (66.7%), scaring devices (66.0%), destruction of breeding colonies (33.3%), taking no action (26.3%), and killing birds (20.7%). Taking no action was more endorsed by the general public, while scaring, colony destruction, and killing were more endorsed by fishers. Nets for cover were the most prioritized management strategy among all residents (47.3%), followed by compensation (29.3%), scaring (11.4%), taking no action (6.0%), colony destruction (4.0%), and killing (2.0%). Fishers prioritized nets for cover, colony destruction, and killing more than the general public, who prioritized taking no action, compensation, and scaring. These findings will be valuable for informing the management process of the great cormorant–fishery conflict.
Full article
(This article belongs to the Section Biodiversity Conservation)
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Open AccessArticle
Functional Diversity and Ecosystem Services of Birds in Productive Landscapes of the Colombian Amazon
by
Jenniffer Tatiana Díaz-Cháux, Alexander Velasquez-Valencia, Alejandra Martínez-Salinas and Fernando Casanoves
Diversity 2025, 17(5), 305; https://doi.org/10.3390/d17050305 - 23 Apr 2025
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The expansion of anthropogenic activities drives changes in the composition, structure, and spatial configuration of natural landscapes, influencing both the taxonomic and functional diversity of bird communities. This pattern is evident in the Colombian Amazon, where agricultural and livestock expansion has altered ecological
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The expansion of anthropogenic activities drives changes in the composition, structure, and spatial configuration of natural landscapes, influencing both the taxonomic and functional diversity of bird communities. This pattern is evident in the Colombian Amazon, where agricultural and livestock expansion has altered ecological dynamics, avifaunal assemblages, and the provision of regulating ecosystem services. This study analyzed the influence of agroforestry (cocoa-based agroforestry systems—SAFc) and silvopastoral systems (SSP) on the functional diversity of birds and their potential impact on ecosystem services in eight productive landscape mosaics within the Colombian Amazon. Each mosaic consisted of a 1 km2 grid, within which seven types of vegetation cover were classified, and seven landscape metrics were calculated. Bird communities were surveyed through visual observations and mist-net captures, during which functional traits were measured. Additionally, functional guilds were assigned to each species based on a literature review. Five multidimensional indices of functional diversity were computed, along with community-weighted means per guild. A total of 218 bird species were recorded across both land-use systems. Bird richness, abundance, and functional diversity—as well as the composition of functional guilds—varied according to vegetation cover. Functional diversity increased in mosaics containing closed vegetation patches with symmetrical configurations. Variations in functional guilds were linked to low functional redundancy, which may also lead to differences in the provision of regulating ecosystem services such as biological pest control and seed dispersal—both of which are critical for the regeneration and connectivity of productive rural landscapes. In conclusion, functional diversity contributes to the resilience of bird communities in landscapes with Amazonian agroforestry and silvopastoral systems, highlighting the need for landscape management that promotes structural heterogeneity to sustain regulating ecosystem services and ecological connectivity.
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Open AccessArticle
The Distribution, Similarity, and Conservation Status of the Amphibians and Reptiles of the Biogeographic Provinces of Northwestern Mexico
by
Julio A. Lemos-Espinal and Geoffrey R. Smith
Diversity 2025, 17(5), 304; https://doi.org/10.3390/d17050304 - 22 Apr 2025
Abstract
The herpetofaunal diversity of northwestern Mexico, encompassing the Californian, Baja California, and Sonoran Desert biogeographic provinces, is characterized by relatively low species richness but a high degree of endemism. This region, particularly the islands of the Gulf of California, is recognized as a
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The herpetofaunal diversity of northwestern Mexico, encompassing the Californian, Baja California, and Sonoran Desert biogeographic provinces, is characterized by relatively low species richness but a high degree of endemism. This region, particularly the islands of the Gulf of California, is recognized as a biodiversity hotspot. However, amphibian and reptile species in this area, especially those on the Gulf islands, are facing significant threats to their survival, including non-native species, habitat loss, and climate change. These factors pose considerable challenges to their conservation. In this study, we compiled updated lists of amphibian and reptile species across these three provinces, assessed their conservation status, identified the threats they face, and compared them with those in neighboring biogeographic provinces. Our analysis recorded a total of 228 species, comprising 29 amphibians and 199 reptiles, with a notable proportion of endemics, particularly in Baja California. Amphibians, due to the region’s arid conditions, are underrepresented and generally face a lower level of conservation concern. In contrast, reptiles, especially those on the islands, are at higher risk, primarily due to habitat loss, invasive species, and climate change. Of the 228 species, one amphibian and 21 reptiles are classified in a category of conservation concern (Vulnerable, Endangered, Critically Endangered) by the International Union for Conservation of Nature (IUCN) Red List. In addition, 47 species (one amphibian and 46 reptiles) are listed as threatened (A) or at risk of extinction (P) by the Secretaría del Medio Ambiente y Recursos Naturales (SEMARNAT). These findings emphasize the urgent need for conservation strategies, particularly for island reptile species, that involve collaboration among scientists, local communities, and federal and state authorities to address these threats and safeguard the region’s herpetofauna. Similarity analysis revealed significant ecological connectivity among the amphibian and reptile communities across the three provinces. The similarity in species between the Californian, Baja California, and Sonoran Desert provinces highlights the role of historical climatic events, geographic barriers, and ecological factors in shaping species distributions of amphibians and reptiles in northwestern Mexico.
Full article
(This article belongs to the Special Issue Animal Diversity Hot Topics in 2025)
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Open AccessArticle
Denser Mitogenomic Sampling for Exploring the Phylogeny of Tellinoidea (Mollusca: Bivalvia)
by
Weikang Tang, Tao Xu, Jihang Gong and Lingfeng Kong
Diversity 2025, 17(5), 303; https://doi.org/10.3390/d17050303 - 22 Apr 2025
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The superfamily Tellinoidea is one of the most diverse groups of marine bivalves, with significant ecological and economic value. To date, the availability of complete mitochondrial genome data within Tellinoidea remains limited, and the taxonomic coverage is still insufficient to resolve its internal
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The superfamily Tellinoidea is one of the most diverse groups of marine bivalves, with significant ecological and economic value. To date, the availability of complete mitochondrial genome data within Tellinoidea remains limited, and the taxonomic coverage is still insufficient to resolve its internal controversies. The current study aims to further explore the phylogenetic relationships within Tellinoidea through denser sampling. We have newly sequenced the mitochondrial genomes of 13 species, among which seven genera are being published for the first time. Combined with the published mitogenomes and transcriptomic data, we constructed the most comprehensive Tellinoidea phylogeny to date through maximum likelihood and Bayesian Inference analyses. Our findings support the monophyly of the superfamily Tellinoidea, with Semelidae nesting as a monophyletic group within Tellinidae. We also support the paraphyly of Tellinidae based on the mitochondrial genome data for the first time, identifying that the two subfamilies (Macominae and Tellininae) are polyphyletic. Gene rearrangement analysis reveals a relatively high degree of variation in Semelidae. By expanding the mitochondrial genome dataset, this study provides new insights into the phylogeny of Tellinoidea and underscores the need for further sampling of species to reassess the phylogenetic relationships of Tellinidae and the entire Tellinoidea.
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Open AccessArticle
Polychaetes Associated with Calcareous Red Algae Corallina officinalis in the Northern Adriatic Sea
by
Valentina Pitacco, Moira Buršić, Ante Žunec, Petra Burić, Neven Iveša, Ines Kovačić, Emina Pustijanac, Ljiljana Iveša, Tanja Vojvoda Zeljko and Borut Mavrič
Diversity 2025, 17(5), 302; https://doi.org/10.3390/d17050302 - 22 Apr 2025
Abstract
Polychaetes are important marine invertebrates that contribute to sediment bioturbation, nutrient recycling, and food web dynamics. This study examines the diversity and structure of the polychaete assemblages associated with the red algae Corallina officinalis in areas with different levels of anthropogenic pressures of
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Polychaetes are important marine invertebrates that contribute to sediment bioturbation, nutrient recycling, and food web dynamics. This study examines the diversity and structure of the polychaete assemblages associated with the red algae Corallina officinalis in areas with different levels of anthropogenic pressures of the Northeastern Adriatic Sea. Sampling was performed in the intertidal zones. Altogether, 54 species from 13 families were found, with Syllidae being the most abundant. Polychaete richness, relative abundance and diversity at sampling locations with and without anthropogenic pressures showed no significant difference. Multivariate analyses revealed significant differences in species composition between sites under anthropogenic pressures and those without, with key species such as Sphaerosyllis pirifera, Syllis rosea, Syllis prolifera, Syllis gerundensis, and Platynereis dumerilii playing significant roles. Syllis rosea was the most abundant in locations without anthropogenic pressures, while S. pirifera was the most abundant in locations under anthropogenic pressures. These results suggest that while polychaete communities are resilient, anthropogenic pressures are causing shifts in species composition. This pattern is consistent with the results of related studies, indicating a broader ecological trend. The shifts observed here should raise concern among conservation ecologists, underscoring the importance of long-term monitoring to understand and mitigate the impacts of human activities on coastal ecosystems.
Full article
(This article belongs to the Section Marine Diversity)
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Open AccessArticle
How Does Extreme Drought Affect Phytoplankton Community Assembly in Aquatic Reserves? A Study from the Confluence of Poyang Lake and Yangtze River, China
by
Yufei Jiang, Wenting Shen, Lei Fang, Bao Zhang, Chiping Kong, Wei Zhang and Qun Xu
Diversity 2025, 17(4), 301; https://doi.org/10.3390/d17040301 - 21 Apr 2025
Abstract
Extreme drought events, intensified by climate change, critically threaten aquatic ecosystem stability by restructuring phytoplankton communities. However, the mechanisms underlying drought-driven community assembly remain poorly understood. This study investigated the impacts of extreme drought on phytoplankton community dynamics in the aquatic reserves of
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Extreme drought events, intensified by climate change, critically threaten aquatic ecosystem stability by restructuring phytoplankton communities. However, the mechanisms underlying drought-driven community assembly remain poorly understood. This study investigated the impacts of extreme drought on phytoplankton community dynamics in the aquatic reserves of Jiujiang City, China, a critical ecotone of the Yangtze River and Poyang Lake. Through multi-temporal sampling (2022–2023) across 12 sites, we integrated taxonomic, functional group, and co-occurrence network analyses with environmental driver assessments. The results revealed that extreme drought significantly reduced phytoplankton species diversity and triggered a functional shift from disturbance-adapted (e.g., MP group) to pollution-tolerant taxa (e.g., W1 group). Deterministic processes dominated community assembly, driven by drought-induced environmental filtering through water temperature, dissolved oxygen, and nutrient fluctuations. Copper emerged as a key stressor, correlating with the abundance of Cryptophyta. Co-occurrence networks, cohesion, and robustness exhibited heightened complexity and stability under extreme drought, emphasizing stress-induced mutualistic interactions. Our findings elucidate how drought reshapes phytoplankton communities via nutrient dynamics and deterministic species interactions, offering critical insights for managing aquatic ecosystems under escalating climatic extremes.
Full article
(This article belongs to the Special Issue Studies on Biodiversity and Ecology of Algae in China—2nd Edition)
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Open AccessCommunication
Differential Accumulation of Particulate Pollutants in Gills and Gastrointestinal Tracts in Sphoeroides Fish from Tropical and Subtropical Estuaries in Brazil
by
Sérgio Murilo de Souza Filho, Marco Tadeu Grassi, Mayara Padovan dos Santos, Juliano Morimoto, Marcelo Soeth and Luís Fernando Fávaro
Diversity 2025, 17(4), 300; https://doi.org/10.3390/d17040300 - 21 Apr 2025
Abstract
The widespread use of recyclable materials in contemporary society has led to the accumulation of pollutants in estuaries and marine ecosystems, with potential impacts on biodiversity. This study assessed the abundance and types of particulate pollutants in Sphoeroides fish across two Brazilian estuaries
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The widespread use of recyclable materials in contemporary society has led to the accumulation of pollutants in estuaries and marine ecosystems, with potential impacts on biodiversity. This study assessed the abundance and types of particulate pollutants in Sphoeroides fish across two Brazilian estuaries (tropical and subtropical). Our findings showed that 70 biological samples from fish (92.11%) contained debris, with the tropical estuary exhibiting the highest abundance (n = 499 particles—67% of the total), dominated by laminar (film) particulate pollutants (76.75%). In this estuary, the gill exhibited the highest contamination index, with most particulate pollutants (<1 mm and 1–3 mm) found in 63.6% and 54.5% of samples, respectively. In the subtropical estuary, 246 debris particles (33% of the total) were detected in the biological samples, with 58.5% of particles being of the film type. The gastrointestinal tract had the highest contamination index in this region, with 70.6% of particles in the 1–3 mm size range. These results highlight the pervasive presence of particulate pollutants in estuarine ecosystems and the organ-specific contamination patterns in tropical and subtropical regions, underscoring the ecological risks posed by plastic waste to estuarine biodiversity in hotspot regions.
Full article
(This article belongs to the Special Issue Socioecology and Biodiversity Conservation—2nd Edition)
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Open AccessArticle
Microbial Carbonates of Upper Triassic Doi Long Formation, Lampang Group: A Study of New Outcrop Localities in Northern Lampang, Central North Thailand
by
Kritsada Moonpa, Mongkol Udchachon, Jirattikarn Jainanta and Sathit Kanthata
Diversity 2025, 17(4), 299; https://doi.org/10.3390/d17040299 - 21 Apr 2025
Abstract
The Doi Long Formation is the youngest record of Triassic deposits of the Lampang Group, Sukhothai Zone, central northern Thailand. The evolutionary history behind the presence of microbial limestone remains unknown, despite its high diversity and abundance in reef environments. This study documents
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The Doi Long Formation is the youngest record of Triassic deposits of the Lampang Group, Sukhothai Zone, central northern Thailand. The evolutionary history behind the presence of microbial limestone remains unknown, despite its high diversity and abundance in reef environments. This study documents new observations on the microbial carbonates of the Doi Long Formation in the northern Lampang Province. Eight new outcrop localities have been discovered and are analyzed in terms of sedimentological details. Microfacies description and fossil identification allow us to interpret the specific depositional environments of the bioconstructors of these mound-like build-ups. In the study localities, the Doi Long Formation consists of predominantly microbial carbonates, containing microproblematic organisms, microbialites (stromatolites), algae, and sponges. Five main microfacies are distinguished and several microproblematic organisms have been identified. The study localities yield microencrusters and microproblematica fossils, including Girvanella-like microstructures, Cladogirvanella, Garwoodia, Cayeuxia, Tubiphytes-like microorganisms, and shell fragments. Depositional environments corresponding to reef (mound-like microbial/microbialite build-ups) settings and lagoon environments have been proposed. Understanding the diversity within the Doi Long Formation is essential for clarifying the fossil assemblage and biological processes in reef ecosystems during the Late Triassic.
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(This article belongs to the Section Microbial Diversity and Culture Collections)
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Open AccessArticle
The Quantification of Morphological Variation and Development of Morphology-Based Keys to Identify Species of Fusconaia and Pleurobema (Unionidae) in the Green River, Kentucky, USA
by
Miluska Olivera-Hyde, Jess W. Jones and Eric M. Hallerman
Diversity 2025, 17(4), 298; https://doi.org/10.3390/d17040298 - 21 Apr 2025
Abstract
We quantified morphological variation among genetically identified specimens of Fusconaia flava, F. subrotunda, Pleurobema cordatum, P. plenum, P. sintoxia, and P. rubrum inhabiting the Green River, Kentucky, species with shells that are morphologically similar to each other and
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We quantified morphological variation among genetically identified specimens of Fusconaia flava, F. subrotunda, Pleurobema cordatum, P. plenum, P. sintoxia, and P. rubrum inhabiting the Green River, Kentucky, species with shells that are morphologically similar to each other and thus difficult to identify. Molecular identifications then were compared with phenotype-based identifications by experts, who on average correctly identified 70% of the specimens. Expert identification of the putative species P. rubrum and P. sintoxia resulted in them usually being identified as the latter. Multi-variable decision tree analysis was conducted to determine the best suite of morphological variables for identifying live mussels and shells to species. Cross-validation error rates for these analyses were 12.6% and 4.14% for live mussels and shells, respectively. Both random forest and decision tree analyses showed the most important variables to be the presence/absence of a sulcus and shell shape (trapezoidal, circular, oval, equilateral triangle, or isosceles triangle). Dichotomous keys for identifying shells and live mussels were developed based on key morphological characteristics readily identifiable in the field, including foot color, beak direction, and beak position relative to the anterior margin. However, a definitive identification of these species may still need to rely on molecular methods, especially for endangered species.
Full article
(This article belongs to the Special Issue Advances in Freshwater Mollusk Research)
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Open AccessArticle
First Genomic Survey of Pleurocryptella fimbriata Provides Preliminary Insights into Genome Characteristics and Evolution of a Deep-Sea Parasitic Isopod
by
Aiyang Wang, Min Hui and Zhongli Sha
Diversity 2025, 17(4), 297; https://doi.org/10.3390/d17040297 - 20 Apr 2025
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Genomic adaptations of parasitic crustaceans in deep-sea extreme environments are poorly understood. This study presents the first genome survey of Pleurocryptella fimbriata, a bopyrid isopod parasitizing deep-sea squat lobsters, using Illumina sequencing. The genome size was estimated to be 1.06 Gb via
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Genomic adaptations of parasitic crustaceans in deep-sea extreme environments are poorly understood. This study presents the first genome survey of Pleurocryptella fimbriata, a bopyrid isopod parasitizing deep-sea squat lobsters, using Illumina sequencing. The genome size was estimated to be 1.06 Gb via a K-mer analysis, smaller than its free-living relatives. The repeat content and heterozygosity were 66.31% and 1.14%, respectively, indicating a complex genome. The draft genome assembly yielded 0.93 Gb of scaffolds with an N50 length of 989 bp, and a complete mitochondrial genome of 14,711 bp was obtained. Phylogenetic analyses of 13 mitochondrial protein-coding genes confirmed the monophyly of Bopyridae, supporting Pleurocryptella as the most primitive genus within the group and the key role of deep sea in the origin and diversification of bopyrids. A mitochondrial gene variation analysis identified NAD2 and NAD4 as promising DNA markers for a population genetic study of P. fimbriata. Twenty-four positively selected sites across COX1, NAD2, and NAD4 genes in P. fimbriata explained the genetic basis of its adaptive evolution at the mitochondrial level. These findings provide valuable genomic resources for deep-sea parasitic crustaceans and establish a foundation for further high-quality genome assembly and adaptive mechanism studies of P. fimbriata.
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Open AccessReview
Introduction of Non-Native Fish Species in Red Sea Aquaculture: Implications for Marine Ecosystem Integrity
by
Seemab Zehra, Pranav Pulukkayi, Mahalakshmi Boopathi, Fadiyah Baalkhuyur, Mohammed Alghamdi, Ali Al Shaikhi, Youssef S. Alhafedh and Asaad H. Mohamed
Diversity 2025, 17(4), 296; https://doi.org/10.3390/d17040296 - 20 Apr 2025
Abstract
Aquaculture is a rapidly expanding industry that holds significant potential to meet growing seafood demands and it is expected to alleviate pressures on wild stocks. The use of non-native fishes has been practiced worldwide as a strategy to enhance production and to promote
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Aquaculture is a rapidly expanding industry that holds significant potential to meet growing seafood demands and it is expected to alleviate pressures on wild stocks. The use of non-native fishes has been practiced worldwide as a strategy to enhance production and to promote financial sustainability in aquaculture. However, the introduction and cultivation of non-native species (hereafter, NNS) in aquaculture can pose severe risks to marine ecosystems, particularly in biodiverse regions like the Red Sea. This review explores insights about commercially produced species, the rationale of introducing NNS, and the potential associated risks, focusing on escapees, genetic pollution, and competition with native species, disease transmission, and habitat modification. The review also highlights the ecological consequences of such risks and proposes strategies to mitigate their impacts, emphasizing the need for comprehensive monitoring, regulatory frameworks, and sustainable aquaculture practices to safeguard marine ecosystem integrity in the region.
Full article
(This article belongs to the Section Marine Diversity)
Open AccessArticle
Patterns of Insect Distribution in Fruit Trees of South Romania and Their Role as Bacterial Vectors
by
Dana S. Copoiu, Paris Lavin, Corina Itcus and Cristina Purcarea
Diversity 2025, 17(4), 295; https://doi.org/10.3390/d17040295 - 20 Apr 2025
Abstract
This study is the first investigation of tree–insect–bacteria interactions in southern Romania, documenting the distribution of 19 insect species across various fruit trees and their insect-associated bacterial diversity. Insect species were identified through DNA barcoding, while bacterial communities in Anthomyia, Botanophila,
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This study is the first investigation of tree–insect–bacteria interactions in southern Romania, documenting the distribution of 19 insect species across various fruit trees and their insect-associated bacterial diversity. Insect species were identified through DNA barcoding, while bacterial communities in Anthomyia, Botanophila, Drosophila, and Scaptomyza insects were analyzed via 16S rRNA gene sequencing. Insect diversity varied across apple, cherry, plum, peach, and quince trees, with most species showing tree-specific distribution, except for Drosophila melanogaster, which was found on all tree species. Its presence was primarily influenced by fruit development stages rather than temperature changes. Insect bacterial communities comprised 51 genera across four phyla, predominantly Pseudomonadota and Bacillota, that varied by tree species rather than insect species, suggesting the potential role of these flies as bacterial vectors. Several potential pathogenic bacterial genera were identified as biomarkers within insect microbiomes, suggesting their involvement in disease transmission, particularly affecting apple and cherry trees. This study also provides the first report of seven insect species in Romania, being the first microbiome characterization of four dipteran species associated with regional fruit trees.
Full article
(This article belongs to the Special Issue Microbiota Diversity in Plants and Forest—2nd Edition)
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Open AccessArticle
Integrative Description of Temnothorax siculus sp. n.: A New Ant Species from Sicily, Italy (Hymenoptera, Formicidae)
by
Enrico Schifani, Antonio Alicata, Matthew M. Prebus and Sándor Csősz
Diversity 2025, 17(4), 294; https://doi.org/10.3390/d17040294 - 19 Apr 2025
Abstract
The mostly Holarctic genus Temnothorax (Hymenoptera, Formicidae) is the most diverse ant genus in temperate regions. The Mediterranean, a biodiversity hotspot of rare ant species, hosts over 150 Temnothorax taxa, including several short-range endemics. Over the last few years, phylogenomic reconstructions and integrative
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The mostly Holarctic genus Temnothorax (Hymenoptera, Formicidae) is the most diverse ant genus in temperate regions. The Mediterranean, a biodiversity hotspot of rare ant species, hosts over 150 Temnothorax taxa, including several short-range endemics. Over the last few years, phylogenomic reconstructions and integrative taxonomy have significantly improved the understanding of global Temnothorax diversity, but much taxonomic work is still needed in the Mediterranean region. Here, we present the integrative description of a new species of the genus, discovered in the central Mediterranean island of Sicily: Temnothorax siculus sp. n. is defined and compared to congeneric species integrating morphometrics and phylogenomics. It is a ground-nesting, lowland species, of which workers were regularly observed foraging on bushes and small trees. In the global phylogeny, covering all the main lineages of the region, it belongs to the Palearctic clade and is related to the tuberum and unifasciatus complexes. Morphological separation from other Sicilian Temnothorax species can generally be achieved on qualitative characters, but we also provide morphometric discriminant functions to separate it from T. apenninicus and especially T. unifasciatus. Temnothorax siculus has been rarely collected but appears to be widespread in Sicily, and may occur in neighboring regions.
Full article
(This article belongs to the Special Issue Systematics, Evolution and Diversity in Ants)
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Open AccessArticle
Biostratigraphy, Paleoenvironments, and Paleobiogeography of the Middle–Upper Eocene Ostracods from Northwestern and Northeastern Banks of the Nile Valley, Egypt
by
Safaa Abu Bakr, Ibrahim M. Abd El-Gaied, Mostafa M. Sayed, Petra Heinz, Michael Wagreich and Abdelaziz Mahmoud
Diversity 2025, 17(4), 293; https://doi.org/10.3390/d17040293 - 19 Apr 2025
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The middle and upper Eocene sedimentary successions exposed along the northwestern and northeastern portions of the Nile Valley, Egypt, have been thoroughly examined for their ostracod assemblages. This study enhances the understanding of biostratigraphic zonations and evaluates the paleobiogeographic distribution and paleoenvironmental conditions
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The middle and upper Eocene sedimentary successions exposed along the northwestern and northeastern portions of the Nile Valley, Egypt, have been thoroughly examined for their ostracod assemblages. This study enhances the understanding of biostratigraphic zonations and evaluates the paleobiogeographic distribution and paleoenvironmental conditions that prevailed during the deposition of this sedimentary record. Lithostratigraphically, the studied successions are subdivided into four stratigraphic units, arranged in ascending order as follows: the Qarara, the El Fashn, the Gehannam, and the Beni Suef formations. A total of 125 rock samples were selected and well analyzed, resulting in the identification of sixty-five ostracod species and subspecies belonging to thirty-three genera, fifteen families, and three superfamilies. The stratigraphic distribution of the recorded ostracod taxa contributed to the construction of four local biozones, spanning the interval from the upper Lutetian to lower Priabonian: Schizocythere fadlensis Zone (upper Lutetian–lower Bartonian), Loxoconcha pseudopunctatella Zone, Dygmocythere ismaili Zone (Bartonian), and Asymmetricythere hiltermanni Zone (Bartonian–Priabonian). These biozones are well described, discussed, and correlated with those previously documented in different areas of Egypt and neighboring countries. The statistical analysis, supported by ternary plot diagrams, indicates that the depositional environments of the studied rock units fluctuated between shallow inner neritic and deeper outer neritic marine environments. The identified taxa display a wide geographic distribution and show a significant similarity with those identified in the southern, northern, and eastern Tethyan provinces, suggesting a direct marine connection during the Eocene.
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Open AccessArticle
Influence of Natural and Artificial Habitats and Microhabitats on Urban Amphibian Diversity and Behavior
by
Rocio Pamela Demartín, Romina Ghirardi and Javier Alejandro López
Diversity 2025, 17(4), 292; https://doi.org/10.3390/d17040292 - 19 Apr 2025
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Species presence in urban landscapes is driven by complex biological and environmental interactions. In this study, we evaluated habitat and microhabitat selection by amphibians in urban environments using correspondence analysis, multiple correspondence analysis, and preference analysis. Data on habitats, microhabitats, and activities were
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Species presence in urban landscapes is driven by complex biological and environmental interactions. In this study, we evaluated habitat and microhabitat selection by amphibians in urban environments using correspondence analysis, multiple correspondence analysis, and preference analysis. Data on habitats, microhabitats, and activities were recorded for 26 amphibian species in urban areas. All species were observed in natural habitats, while only 11 in artificial habitats. Leptodactylus latinasus, Leptodactylus macrosternum, Rhinella arenarum, and Rhinella dorbignyi were found in both habitat types, in both aquatic and terrestrial environments. Most individuals (74%) were recorded in natural habitats, predominantly aquatic ones. In artificial habitats (26%), R. arenarum was the most abundant species, primarily using terrestrial habitats. All species exhibited some degree of habitat preference, even generalist species. Amphibian activities were also linked to habitat type, with natural aquatic habitats primarily used for breeding and natural terrestrial habitats for refuge, foraging, and other activities. Our results highlight that heterogeneous natural habitats promote greater species diversity, while artificial habitats restrict amphibian presence. However, the capacity of certain species to adjust to artificial environments underscores the need to enhance these habitats by adding bodies of water, bare ground, and vegetation of all kinds to support the conservation of urban amphibians.
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Open AccessArticle
The Evolution of Mosasaurid Foraging Behavior Through the Lens of Stable Carbon Isotopes
by
Michael J. Polcyn, John A. Robbins, Anne S. Schulp, Johan Lindgren and Louis L. Jacobs
Diversity 2025, 17(4), 291; https://doi.org/10.3390/d17040291 - 19 Apr 2025
Abstract
A large data set of new and previously published measurements of δ13C values derived from tooth enamel (n = 223, of which 93 are new) are compiled to explore patterns of foraging area preferences of Late Cretaceous mosasaurid squamates over
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A large data set of new and previously published measurements of δ13C values derived from tooth enamel (n = 223, of which 93 are new) are compiled to explore patterns of foraging area preferences of Late Cretaceous mosasaurid squamates over evolutionary time scales (~93–66 Ma). Our results indicate that small-bodied halisaurines are restricted to a relatively nearshore range, overlapping the lower end of the range of plioplatecarpines and some mosasaurine taxa. Most moderately sized plioplatecarpines occupy a relatively narrow foraging area in much of the nearshore and proximal offshore marine foraging area for the majority of their existence. Tylosaurines exhibit a greater offshore marine range than plioplatecarpines, consistent with their large body size and the robustness of their feeding apparatus. The largest tylosaurine taxa are replaced by Mosasaurus in the Late Campanian–Maastrichtian in the offshore foraging range. Mosasaurine taxa are found to occupy the broadest range of foraging areas, but their ranges are taxonomically segregated, consistent with adult body size and the diversity of feeding adaptations such as tooth morphologies and skull architecture seen in that subfamily. Where foraging areas of multiple taxa overlap, differences are typically in tooth form, reflecting prey preference or feeding niche. Foraging area occupation by multiple taxa with similar tooth forms suggests that other factors such as body size and prey acquisition style may have allowed for the finer partitioning of resources. Deep diving and long submergence may have also contributed to the depleted signals recovered for some of the large-bodied durophages and the largest of the macrophagous apex predators.
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(This article belongs to the Special Issue Making New Out of the Old: Recent Biological Advances on Mesozoic Marine Reptiles)
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Open AccessArticle
Habitat and Predator Influences on the Spatial Ecology of Nine-Banded Armadillos
by
Robert C. Lonsinger, Ben P. Murley, Daniel T. McDonald, Christine E. Fallon and Kara M. White
Diversity 2025, 17(4), 290; https://doi.org/10.3390/d17040290 - 19 Apr 2025
Abstract
Mesopredator suppression has implications for community structure, biodiversity, and ecosystem function, but mesopredators with physical defenses may not avoid apex predators. We investigated nine-banded armadillos (Dasypus novemcinctus) in southwestern Oklahoma (USA) to evaluate if a species with physical defenses was influenced
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Mesopredator suppression has implications for community structure, biodiversity, and ecosystem function, but mesopredators with physical defenses may not avoid apex predators. We investigated nine-banded armadillos (Dasypus novemcinctus) in southwestern Oklahoma (USA) to evaluate if a species with physical defenses was influenced by a dominant predator, the coyote (Canis latrans). We sampled nine-banded armadillos and coyotes with motion-activated cameras. We used single-species and conditional two-species occupancy models to assess the influences of environmental factors and coyotes on nine-banded armadillo occurrence and site-use intensity (i.e., detection). We used camera-based detections to characterize the diel activity of each species and their overlap. Nine-banded armadillo occupancy was greater at sites closer to cover, with lower slopes, and further from water, whereas coyote space use was greater at higher elevations; both species were positively associated with recent burns. Nine-banded armadillo occurrence was not influenced by coyotes, but site-use intensity was suppressed by the presence of coyotes. Nine-banded armadillos (strictly nocturnal) and coyotes (predominantly nocturnal) had a high overlap in summer diel activity. Nine-banded armadillos are ecosystem engineers but are often considered a threat to species of concern and/or a nuisance. Thus, understanding the role of interspecific interactions on nine-banded armadillos has important implications for conservation and management.
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(This article belongs to the Special Issue Ecology, Behavior, and Conservation of Armadillos)
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Open AccessArticle
High Diversity of Waters, Communities and Stressors—Design of Croatian Fish Index for Rivers (CFIR)
by
Ivana Buj, Perica Mustafić, Lucija Ivić, Sara Pleše, Zoran Marčić, Davor Zanella, Marko Ćaleta, Sven Horvatić and Lucija Onorato
Diversity 2025, 17(4), 289; https://doi.org/10.3390/d17040289 - 19 Apr 2025
Abstract
Determination of the ecological state of any water body is crucial for its adequate conservation and is, moreover, required by the Water Framework Directive. Employment of multimetric indices, which integrate various anthropogenic threats to water systems with the responses of different elements of
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Determination of the ecological state of any water body is crucial for its adequate conservation and is, moreover, required by the Water Framework Directive. Employment of multimetric indices, which integrate various anthropogenic threats to water systems with the responses of different elements of fish communities, improve our understanding and allow us to monitor the ecological state of freshwater bodies. However, design of multimetric indices that describe the ecological state of water bodies based on fish communities has already proved difficult for the Mediterranean region for several reasons, including the frequent presence of species-poor and ecologically tolerant fish communities, high endemicity and a serious lack of localities with undisturbed fish communities. The development of an ecological state index for natural water bodies in Croatia based on fish as a biological element which we present in this paper was also hindered by similar obstacles, particularly due to exceptionally high endemicity present in rivers of the Adriatic watershed, the great number of distinct water types, and significant anthropogenic pressures. Nevertheless, based on comprehensive sampling of fish communities throughout Croatia and following appropriate statistical procedures, we were able to identify stressors acting on certain water bodies, as well as responses of fish communities to them, and, finally, describe the ecological state of natural water bodies throughout Croatia. We also propose measures that will most likely help in achieving improvement and/or maintenance of the ecological states of these water bodies.
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(This article belongs to the Special Issue Evolution, Systematic and Conservation of Freshwater Fishes)
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Open AccessArticle
Influence of Gall-Inducing Insects (Cynipini) on the Nutritional and Defensive Chemical Profile of Quercus rugosa
by
Miriam Serrano-Muñoz, Elgar Castillo-Mendoza, Alejandro Zamilpa, Patricia Mussali-Galante, Juli Pujade-Villar, Leticia Valencia-Cuevas and Efraín Tovar-Sánchez
Diversity 2025, 17(4), 288; https://doi.org/10.3390/d17040288 - 18 Apr 2025
Abstract
Analyzing the nutritional and defensive chemistry of Quercus rugosa provides insight into gall wasp interactions. Quercus rugosa is the most widely distributed white oak species in Mexico. It is the dominant canopy species in its geographic distribution range and has the largest number
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Analyzing the nutritional and defensive chemistry of Quercus rugosa provides insight into gall wasp interactions. Quercus rugosa is the most widely distributed white oak species in Mexico. It is the dominant canopy species in its geographic distribution range and has the largest number of associated gall wasp species (Cynipidae: Cynipini). Our main aims were to characterize the nutritional and defensive chemistry of Q. rugosa leaves and determine whether this chemistry differed between leaves with and without galls. We evaluated 60 trees from six populations of Q. rugosa in central Mexico. For each tree, we analyzed the nutritional chemistry (nitrogen, phosphorus, carbon, and carbon/nitrogen ratio) and defensive chemistry (secondary metabolites). Also, we characterized the community of cynipids in the leaf tissue of each tree. We documented 18 cynipid species, and the cynipid communities differed in composition among localities. We recorded the presence of a total of ten phenolics. The composition of nutritional and defensive chemicals differed significantly between leaves with and without galls in each locality. The nutritional and defensive chemical compounds of Q. rugosa were influenced by their associated cynipids. Our results suggest that gall-inducing cynipids influence the production of secondary metabolites in leaves with galls through the reassignment of nutritional compounds by the hosts.
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(This article belongs to the Section Biodiversity Conservation)
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