Trichotorquatus salinus sp. nov. (Oculatellaceae, Cyanobacteria) from a Saltern of Gomso, Republic of Korea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collections and Cultures
2.2. Morphological Analysis and Characterization
2.3. DNA Extraction, PCR, and Sequencing
2.4. Phylogenetic Analyses and Molecular Distance Analyses
2.5. ITS Structure Analyses
3. Results
3.1. Taxonomic Treatment and Morphological Characterization
3.2. 16S rRNA and Phylogenetic Affiliation of Trichotorquatus salinus
3.3. Characterization of the 16S–23S ITS Region of Trichotorquatus salinus
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Filaments | Sheath | Trichomes | Apical Cell | Necridia | Hormogonia |
---|---|---|---|---|---|---|
Trichotorquatus salinus | False branched (Scytonema-type, Tolypothrix-type) 2.1–3.4 μm wide | Sometimes absent Long or short collar Within bulging 0.1–0.9 μm thick | Distinctly constricted Regularly or loosely spiral 1.9–2.9 μm wide | Blue-green Rarely tangled 2.3–4.3 μm long | Rows Sections up to 5 μm long | Short Under 10 cells long |
T. andrei | Unbranched 1.6–5.6 μm wide | Sometimes absent 0.2–3.0 μm thick | Distinctly constricted 1.0–2.8 μm wide | Yellowish 1.4–6.3 μm long | Rows Sections up to 11 μm long | Short 4–8 cells long |
T. coquimbo | Unbranched 2.2–3.6 μm wide | Sometimes absent 0.1–0.6 μm thick Rarely short collar | Distinctly constricted 1.8–3.4 μm wide | Pale yellowish green 1.6–5.0 μm long | Rows Sections up to 4.9 μm long | Short 4–10 cells long |
T. ladouxae | Mostly unbranched 2.0–7.0 μm wide | Sometimes absent 0.2–0.3 μm thick | Distinctly constricted 2.0–3.6 μm wide | Blue-green 1.8–4.6 μm long | Rows Sections up to 12 μm long | Short Under 10 cells long |
T. maritimus T | Mostly unbranched 2.2–7.3 μm wide | Sometimes absent Frayed collar 0.2–2.4 μm thick | Distinctly constricted 2.1–4.3 μm wide | Blue-green 2.2–32 μm long | Rows Sections up to 32 μm long | Very short 1–6 cells long |
Trichotorquatussp. 5 | Unbranched 2.0–4.0 μm wide | Sometimes absent 0.2–0.6 μm thick | Indistinctly constricted 2.0–2.8 μm wide | Blue-green | - | Short Under 10 cells long |
T. salinus | T. andrei | T. coquimbo | T. ladouxae | T. maritimus | |
---|---|---|---|---|---|
T. salinus | 0.00–0.30% | ||||
T. andrei | 55.31–57.34% | 0.00–1.33% | |||
T. coquimbo | 58.52–59.25% | 23.35–24.63% | 0.21% | ||
T. ladouxae | 56.66–58.52% | 12.16–13.22% | 29.57–30.37% | 0.00–1.80% | |
T. maritimus | 59.37% | 13.00–14.36% | 20.66–21.28% | 15.62–16.72% | 0.00–0.22% |
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Lee, N.-J.; Kim, D.-H.; Kim, J.-H.; Lim, A.S.; Lee, O.-M. Trichotorquatus salinus sp. nov. (Oculatellaceae, Cyanobacteria) from a Saltern of Gomso, Republic of Korea. Diversity 2023, 15, 65. https://doi.org/10.3390/d15010065
Lee N-J, Kim D-H, Kim J-H, Lim AS, Lee O-M. Trichotorquatus salinus sp. nov. (Oculatellaceae, Cyanobacteria) from a Saltern of Gomso, Republic of Korea. Diversity. 2023; 15(1):65. https://doi.org/10.3390/d15010065
Chicago/Turabian StyleLee, Nam-Ju, Do-Hyun Kim, Jee-Hwan Kim, An Suk Lim, and Ok-Min Lee. 2023. "Trichotorquatus salinus sp. nov. (Oculatellaceae, Cyanobacteria) from a Saltern of Gomso, Republic of Korea" Diversity 15, no. 1: 65. https://doi.org/10.3390/d15010065