Open AccessArticle
Pharmacokinetics of Chiral Dendrimer-Triamine-Coordinated Gd-MRI Contrast Agents Evaluated by in Vivo MRI and Estimated by in Vitro QCM
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Yuka Miyake 1, Syungo Ishikawa 1, Yu Kimura 1,2, Aoi Son 1, Hirohiko Imai 3, Tetsuya Matsuda 3, Hisatsugu Yamada 1,4,†, Akio Toshimitsu 1,5 and Teruyuki Kondo 1,4,*
1
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan
2
Research and Educational Unit of Leaders for Integrated Medical System, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan
3
Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan
4
Advanced Biomedical Engineering Research Unit, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan
5
Division of Multidisciplinary Chemistry, Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan
†
Present Address: Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima 770-8506, Japan
Cited by 7 | Viewed by 6480
Abstract
Recently, we developed novel chiral dendrimer-triamine-coordinated Gd-MRI contrast agents (Gd-MRI CAs), which showed longitudinal relaxivity (
r1) values about four times higher than that of clinically used Gd-DTPA (Magnevist
®, Bayer). In our continuing study of pharmacokinetic differences derived from
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Recently, we developed novel chiral dendrimer-triamine-coordinated Gd-MRI contrast agents (Gd-MRI CAs), which showed longitudinal relaxivity (
r1) values about four times higher than that of clinically used Gd-DTPA (Magnevist
®, Bayer). In our continuing study of pharmacokinetic differences derived from both the chirality and generation of Gd-MRI CAs, we found that the ability of chiral dendrimer Gd-MRI CAs to circulate within the body can be directly evaluated by
in vitro MRI (7 T). In this study, the association constants (
Ka) of chiral dendrimer Gd-MRI CAs to bovine serum albumin (BSA), measured and calculated with a quartz crystal microbalance (QCM)
in vitro, were found to be an extremely easy means for evaluating the body-circulation ability of chiral dendrimer Gd-MRI CAs. The
Ka values of
S-isomeric dendrimer Gd-MRI CAs were generally greater than those of
R-isomeric dendrimer Gd-MRI CAs, which is consistent with the results of our previous MRI study
in vivo.
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