Optical Oxygen Sensing and Clark Electrode: Face-to-Face in a Biosensor Case Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Materials
2.2. Microfluidic Setup
2.3. Sensor Fabrication
2.4. Model Experiments
2.5. Bioreceptor Formation
2.6. Control of Biofilm Formation
2.7. Confocal Laser Scanning Microscopy (CLSM)
2.8. Determination of BOD by the Standard Dilution Method
3. Results
3.1. Preliminary Experiments without Bioreceptor
3.2. Yeast Bioreceptor Properties
3.3. Application on Model Glucose Solutions
3.4. BOD5 Estimation on Real Samples
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Melnikov, P.V.; Alexandrovskaya, A.Y.; Naumova, A.O.; Arlyapov, V.A.; Kamanina, O.A.; Popova, N.M.; Zaitsev, N.K.; Yashtulov, N.A. Optical Oxygen Sensing and Clark Electrode: Face-to-Face in a Biosensor Case Study. Sensors 2022, 22, 7626. https://doi.org/10.3390/s22197626
Melnikov PV, Alexandrovskaya AY, Naumova AO, Arlyapov VA, Kamanina OA, Popova NM, Zaitsev NK, Yashtulov NA. Optical Oxygen Sensing and Clark Electrode: Face-to-Face in a Biosensor Case Study. Sensors. 2022; 22(19):7626. https://doi.org/10.3390/s22197626
Chicago/Turabian StyleMelnikov, Pavel V., Anastasia Yu. Alexandrovskaya, Alina O. Naumova, Vyacheslav A. Arlyapov, Olga A. Kamanina, Nadezhda M. Popova, Nikolay K. Zaitsev, and Nikolay A. Yashtulov. 2022. "Optical Oxygen Sensing and Clark Electrode: Face-to-Face in a Biosensor Case Study" Sensors 22, no. 19: 7626. https://doi.org/10.3390/s22197626