Next Article in Journal
Development of an Earth-Field Nuclear Magnetic Resonance Spectrometer: Paving the Way for AI-Enhanced Low-Field Nuclear Magnetic Resonance Technology
Previous Article in Journal
Analysis of Gait Kinematics in Smart Walker-Assisted Locomotion in Immersive Virtual Reality Scenario
Previous Article in Special Issue
Paper-Based Microfluidic Analytical Device Patterned by Label Printer for Point-of-Care Blood Glucose and Hematocrit Detection Using 3D-Printed Smartphone Cassette
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sensors
Volume 24
Issue 17
10.3390/s24175536
sensors-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Label-Free Biosensor Based on Particle Plasmon Resonance Coupled with Diffraction Grating Waveguide

by
Wei-Ting Hsu
1,
Yu-Cheng Lin
2,
Huang-Chin Yang
1,
Devesh Barshilia
2,
Po-Liang Chen
2,
Fu-Chun Huang
2,
Lai-Kwan Chau
1,*,
Wen-Hsin Hsieh
2,* and
Guo-En Chang
2,*
1
Department of Chemistry and Biochemistry and Center for Nano Bio-Detection, National Chung Cheng University, Chiayi 62102, Taiwan
2
Department of Mechanical Engineering and Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng University, Chiayi 62102, Taiwan
*
Authors to whom correspondence should be addressed.
Sensors 2024, 24(17), 5536; https://doi.org/10.3390/s24175536
Submission received: 17 July 2024 / Revised: 8 August 2024 / Accepted: 22 August 2024 / Published: 27 August 2024
(This article belongs to the Special Issue Recent Advances in Microfluidics-Integrated Optical Biosensors)
Browse Figures
Versions Notes

Abstract

Particle plasmon resonance (PPR), or localized surface plasmon resonance (LSPR), utilizes intrinsic resonance in metal nanoparticles for sensor fabrication. While diffraction grating waveguides monitor bioaffinity adsorption with out-of-plane illumination, integrating them with PPR for biomolecular detection schemes remains underexplored. This study introduces a label-free biosensing platform integrating PPR with a diffraction grating waveguide. Gold nanoparticles are immobilized on a glass slide in contact with a sample, while a UV-assisted embossed diffraction grating is positioned opposite. The setup utilizes diffraction in reflection to detect changes in the environment’s refractive index, indicating biomolecular binding at the gold nanoparticle surface. The positional shift of the diffracted beam, measured with varying refractive indices of sucrose solutions, shows a sensitivity of 0.97 mm/RIU at 8 cm from a position-sensitive detector, highlighting enhanced sensitivity due to PPR–diffraction coupling near the gold nanoparticle surface. Furthermore, the sensor achieved a resolution of 3.1 × 10−4 refractive index unit and a detection limit of 4.4 pM for detection of anti-DNP. The sensitivity of the diffracted spot was confirmed using finite element method (FEM) simulations in COMSOL Multiphysics. This study presents a significant advancement in biosensing technology, offering practical solutions for sensitive, rapid, and label-free biomolecule detection.
Keywords: label-free biosensing platform; particle plasmon resonance; UV-assisted embossing; diffraction grating; gold nanoparticle surface label-free biosensing platform; particle plasmon resonance; UV-assisted embossing; diffraction grating; gold nanoparticle surface

Share and Cite

MDPI and ACS Style

Hsu, W.-T.; Lin, Y.-C.; Yang, H.-C.; Barshilia, D.; Chen, P.-L.; Huang, F.-C.; Chau, L.-K.; Hsieh, W.-H.; Chang, G.-E. Label-Free Biosensor Based on Particle Plasmon Resonance Coupled with Diffraction Grating Waveguide. Sensors 2024, 24, 5536. https://doi.org/10.3390/s24175536

AMA Style

Hsu W-T, Lin Y-C, Yang H-C, Barshilia D, Chen P-L, Huang F-C, Chau L-K, Hsieh W-H, Chang G-E. Label-Free Biosensor Based on Particle Plasmon Resonance Coupled with Diffraction Grating Waveguide. Sensors. 2024; 24(17):5536. https://doi.org/10.3390/s24175536

Chicago/Turabian Style

Hsu, Wei-Ting, Yu-Cheng Lin, Huang-Chin Yang, Devesh Barshilia, Po-Liang Chen, Fu-Chun Huang, Lai-Kwan Chau, Wen-Hsin Hsieh, and Guo-En Chang. 2024. "Label-Free Biosensor Based on Particle Plasmon Resonance Coupled with Diffraction Grating Waveguide" Sensors 24, no. 17: 5536. https://doi.org/10.3390/s24175536

APA Style

Hsu, W.-T., Lin, Y.-C., Yang, H.-C., Barshilia, D., Chen, P.-L., Huang, F.-C., Chau, L.-K., Hsieh, W.-H., & Chang, G.-E. (2024). Label-Free Biosensor Based on Particle Plasmon Resonance Coupled with Diffraction Grating Waveguide. Sensors, 24(17), 5536. https://doi.org/10.3390/s24175536

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop