Design and Synthesis of a Mitochondria-Targeting Radioprotectant for Promoting Skin Wound Healing Combined with Ionizing Radiation Injury

Round 1

Reviewer 1 Report

The authors are focused on research of the treatment of skin wounds and designed and synthesized mitochondria-targeted protectant to promote the healing of skin irradiated wounds. The are sufficient amount of methods, however I have several comments and questions:

1/The aims are not written clearly. Results included in aims are not necessary.

2/There are formal mistakes such as N2,Ch2Cl2,CO2, numers of cells and some formal mistakes of references.

3/Some corrections of English are necessary, e.g. in lines 201, 209, 444 and 445.

4/Scheme 2 is not seen clearly.

5/In results it is not necessary to repeat procedures, e.g. lines 283, 284. In lines 294-296 there is a repetition of the procedure. B16-F10 cells were not mentioned in materials.

6/Was CY-TMP1 was not tested individually on skin wounds without irradiation?

7/In methods you mentioned EPH, but results of EPR are missing. 

 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The ms describes the application of  a mitochondria-targeting radioprotectant
(CY-TMP1)  for radiation injury-combined wound repair.

The main question is: why the authors utilized a Normal human liver cells (L-02) and not keratinocytes and fibroblasts? Why the authors did not perform a simply scratch wound assay to test the real wound closure potential? and what about extracellular matrix remodelling and deposition?

More details for statistical analysis are requested.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

The comments of the reviewer were answered therefore I recommend accepting the paper in current form.

Reviewer 2 Report

The authors have correctly replied to questions.