Dual Chemotherapeutic Loading in Oxalate Transferrin-Conjugated Polymersomes Incorporated into Chitosan Hydrogels for Site-Specific Targeting of Melanoma Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Stability and Characterization of Polymersomes
2.2. Co-Encapsulation of Drugs in Polymersomes
2.3. Studies of Degradation and Swelling of Chitosan Hydrogels
2.4. Studies on Drug Release from Chitosan Hydrogels
2.5. In Vitro Cytotoxicity Evaluation
3. Materials and Methods
3.1. Materials
3.2. Polymersome Preparation
3.3. Chitosan Hydrogels Preparation and Incorporation of the Polymersomes
3.4. Swelling and Degradation of Chitosan Hydrogels
3.5. Dimensional Stability and Characterization of Polymersomes
3.6. Determination of Encapsulation Parameters
3.7. Drug Quantification
3.8. Drug Release from Chitosan Hydrogels
3.9. In Vitro Cytotoxicity Evaluation
3.10. Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Polymersomes | DOX | VEM | ||
---|---|---|---|---|
DL (%) | EE (%) | DL (%) | EE (%) | |
Ps without Tf | 12.1 ± 3.5 | 35.0 ± 3.0 | 17.3 ± 2.5 | 42.2 ± 4.2 |
Native Tf-Ps | 10.0 ± 2.5 | 32.2 ± 3.5 | 18.1 ± 4.0 | 40.4 ± 5.0 |
Oxalate Tf-Ps | 11.6 ± 3.2 | 33.8 ± 4.5 | 16.3 ± 4.5 | 39.5 ± 4.8 |
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Aranha, M.d.C.; Alencar, L.M.R.; Souto, E.B.; Kamei, D.T.; Lopes, A.M. Dual Chemotherapeutic Loading in Oxalate Transferrin-Conjugated Polymersomes Incorporated into Chitosan Hydrogels for Site-Specific Targeting of Melanoma Cells. Pharmaceuticals 2024, 17, 1177. https://doi.org/10.3390/ph17091177
Aranha MdC, Alencar LMR, Souto EB, Kamei DT, Lopes AM. Dual Chemotherapeutic Loading in Oxalate Transferrin-Conjugated Polymersomes Incorporated into Chitosan Hydrogels for Site-Specific Targeting of Melanoma Cells. Pharmaceuticals. 2024; 17(9):1177. https://doi.org/10.3390/ph17091177
Chicago/Turabian StyleAranha, Mariana de C., Luciana M. R. Alencar, Eliana B. Souto, Daniel T. Kamei, and André M. Lopes. 2024. "Dual Chemotherapeutic Loading in Oxalate Transferrin-Conjugated Polymersomes Incorporated into Chitosan Hydrogels for Site-Specific Targeting of Melanoma Cells" Pharmaceuticals 17, no. 9: 1177. https://doi.org/10.3390/ph17091177