A Potential Role of the CD47/SIRPalpha Axis in COVID-19 Pathogenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Virus Infection
2.3. Western Blot
2.4. qPCR
2.5. Data Acquisition and Analysis
2.6. Literature Review
3. Results
3.1. SARS-CoV-2 Infection Results in Enhanced CD47 Expression
3.2. Increased SIRPα Levels in SARS-CoV-2-Infected Monocytes
3.3. CD47 and COVID-19 Risk Factors
3.3.1. CD47 and Aging
3.3.2. CD47 and Diabetes
3.3.3. CD47 and Obesity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Reference | Link between Aging and/or Hypertension and Increased CD47 Levels |
---|---|
[33] | CD47 downregulation may be involved in the alpha-tocopherol-mediated inhibition of age-associated streptococcus pneumoniae lung infection in mice |
[37] | Blocking thrombospondin-1/CD47 signaling alleviates deleterious effects of aging on tissue responses to ischemia |
[38] | CD47 null mice indicate that CD47 functions as a vasopressor |
[39] | CD47-null mice are leaner—loss of signaling from the TSP1-CD47 system promotes the accumulation of normally functioning mitochondria in a tissue-specific and age-dependent fashion, leading to enhanced physical performance, lower reactive oxygen species production, and more efficient metabolism |
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[41] | TSP1-CD47 signaling is upregulated in clinical pulmonary hypertension and contributes to pulmonary arterial vasculopathy and dysfunction |
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[46] | Increased CD47 expression causes age-associated deterioration in angiogenesis, blood flow, and glucose homeostasis |
[47] | Increased CD47 levels in the lung of a sickle cell disease patient with pulmonary arterial hypertension relative to control tissues |
[48] | Pulmonary hypertension reduced in a CD47-null mouse model of sickle cell disease |
[49] | Anti-CD47 antibodies reversed fibrosis in various organs in mouse models |
Reference | Link between Aging and/ or Hypertension and Increased CD47 Levels |
---|---|
[58] | Hyperglycemia protects CD47 from cleavage |
[59] | Hyperglycemia protects CD47 from cleavage |
[60] | Hyperglycemia protects CD47 from cleavage |
[61] | Hyperglycemia protects CD47 from cleavage |
[62] | CD47 is involved in pathophysiological changes in retinal cells in response to hyperglycemia in cell cultures and rats |
[63] | Elevated CD47 mRNA levels both in the hippocampus and prefrontal cortex of a type-2 diabetes rat model |
[64] | Increased levels of CD47 in epiretinal membranes with active neovascularization in proliferative diabetic retinopathy |
[65] | Increased THBS1/CD47 signaling in bone marrow-derived angiogenic cells in a rat diabetes model |
[66] | Increased diabetes-associated CD47 levels inhibit angiogenesis and wound healing in a diabetes model in rats |
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McLaughlin, K.-M.; Bojkova, D.; Kandler, J.D.; Bechtel, M.; Reus, P.; Le, T.; Rothweiler, F.; Wagner, J.U.G.; Weigert, A.; Ciesek, S.; et al. A Potential Role of the CD47/SIRPalpha Axis in COVID-19 Pathogenesis. Curr. Issues Mol. Biol. 2021, 43, 1212-1225. https://doi.org/10.3390/cimb43030086
McLaughlin K-M, Bojkova D, Kandler JD, Bechtel M, Reus P, Le T, Rothweiler F, Wagner JUG, Weigert A, Ciesek S, et al. A Potential Role of the CD47/SIRPalpha Axis in COVID-19 Pathogenesis. Current Issues in Molecular Biology. 2021; 43(3):1212-1225. https://doi.org/10.3390/cimb43030086
Chicago/Turabian StyleMcLaughlin, Katie-May, Denisa Bojkova, Joshua D. Kandler, Marco Bechtel, Philipp Reus, Trang Le, Florian Rothweiler, Julian U. G. Wagner, Andreas Weigert, Sandra Ciesek, and et al. 2021. "A Potential Role of the CD47/SIRPalpha Axis in COVID-19 Pathogenesis" Current Issues in Molecular Biology 43, no. 3: 1212-1225. https://doi.org/10.3390/cimb43030086