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Article

Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus

by
Tatiana S. Novikova
1,2,
Evgeny A. Ermakov
1,2,*,†,
Elena V. Kostina
1,†,
Alexander N. Sinyakov
1,
Alexey E. Sizikov
1,3,
Georgy A. Nevinsky
1,2 and
Valentina N. Buneva
1,2,*
1
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
2
Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
3
Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Sciences, 630099 Novosibirsk, Russia
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Curr. Issues Mol. Biol. 2023, 45(12), 9887-9903; https://doi.org/10.3390/cimb45120617
Submission received: 2 November 2023 / Revised: 23 November 2023 / Accepted: 6 December 2023 / Published: 8 December 2023
(This article belongs to the Collection Feature Papers in Current Issues in Molecular Biology)
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Abstract

Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in solution by catalytic anti-DNA antibodies from SLE patients. It has been shown that IgG antibodies from SLE patients hydrolyze ODNs more effectively both in solution and on the surface, compared to IgG from healthy individuals. The data obtained indicate a more efficient hydrolysis of ODNs in solution than immobilized ODNs on the surface. In addition, differences in the specificity of recognition and hydrolysis of certain ODNs by anti-DNA antibodies were revealed, indicating the formation of autoantibodies to specific DNA motifs in SLE. The data obtained expand our understanding of the role of anti-DNA antibodies in SLE. Differences in the recognition and hydrolysis of surface-tethered and dissolved ODNs need to be considered in DNA microarray applications.
Keywords: systemic lupus erythematosus; SLE; anti-DNA antibodies; antinuclear antibodies; natural catalytic antibodies; abzymes; recognition; sequence specificity; microarray; DNase I systemic lupus erythematosus; SLE; anti-DNA antibodies; antinuclear antibodies; natural catalytic antibodies; abzymes; recognition; sequence specificity; microarray; DNase I
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MDPI and ACS Style

Novikova, T.S.; Ermakov, E.A.; Kostina, E.V.; Sinyakov, A.N.; Sizikov, A.E.; Nevinsky, G.A.; Buneva, V.N. Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus. Curr. Issues Mol. Biol. 2023, 45, 9887-9903. https://doi.org/10.3390/cimb45120617

AMA Style

Novikova TS, Ermakov EA, Kostina EV, Sinyakov AN, Sizikov AE, Nevinsky GA, Buneva VN. Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus. Current Issues in Molecular Biology. 2023; 45(12):9887-9903. https://doi.org/10.3390/cimb45120617

Chicago/Turabian Style

Novikova, Tatiana S., Evgeny A. Ermakov, Elena V. Kostina, Alexander N. Sinyakov, Alexey E. Sizikov, Georgy A. Nevinsky, and Valentina N. Buneva. 2023. "Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus" Current Issues in Molecular Biology 45, no. 12: 9887-9903. https://doi.org/10.3390/cimb45120617

APA Style

Novikova, T. S., Ermakov, E. A., Kostina, E. V., Sinyakov, A. N., Sizikov, A. E., Nevinsky, G. A., & Buneva, V. N. (2023). Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus. Current Issues in Molecular Biology, 45(12), 9887-9903. https://doi.org/10.3390/cimb45120617

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