Blocking Microglial Proliferation by CSF-1R Inhibitor Does Not Alter the Neuroprotective Effects of Adoptive Regulatory T Cells in 3xTg Alzheimer’s Disease Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Manufacturing and Adoptive Transfer of Tregs
2.3. Drug Treatment and Adoptive Transfer
2.4. Flow Cytometry
2.5. Passive Avoidance Test (PAT)
2.6. Immunohistochemistry Analysis
2.7. RNA Extraction and RT-PCR Assays
2.8. Thy 1.1 Treg Trafficking
2.9. Statistical Analysis
3. Results
3.1. CSF-1R Inhibition Reduced Microglia and Macrophage Population in 4-Month 3xTg-AD Mice
3.2. Adoptive Transfer of Tregs Attenuates Learning and Memory Impairment in GW2580-Treated 3xTg-AD Mice
3.3. Adoptive Transfer of Tregs Attenuates AD Pathology in GW2580-Treated 3xTg-AD Mice
3.4. Adoptive Transfer of Tregs Modulates Microglial Polarization in GW2580-Treated 3xTg-AD Mice
3.5. Adoptively Transferred Tregs Survive up to 112 Days in Several Tissues of 3xTg-AD Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Gene Name | Forward Primer Sequence (5′–3′) | Reverse Primer Sequence (5′–3′) |
---|---|---|
β-actin | GTG CTA TGT TGC TCT AGA CTT CG | ATG CCA CAG GAT TCC ATA CC |
NOS2 | AGG ACA TCC TGC GGC AGC | GCT TTA ACC CCT CCT GTA |
TNF-α | GGC AGG TTC TGT CCC TTT CAC | TTC TGT GCT CAT GGT GTC TTT TCT |
IL-1β | AAG CCT CGT GCT GTC GGA CC | TGA GGC CCA AGG CCA CAG G |
IL-6 | TTC CAT CCA GTT GCC TTC TTG | GGG AGT GGT ATC CTC TGT GAA GTC |
Ym1 | TGG AGG ATG GAA GTT TGG AC | GAG TAG CAG CCT TGG AAT GT |
IL-10 | CAG CCG GGA AGA CAA TAA CTG | CCG CAG CTC TAG GAG CAT GT |
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Park, S.-Y.; Cha, N.; Kim, S.; Chae, S.; Lee, W.-j.; Jung, H.; Bae, H. Blocking Microglial Proliferation by CSF-1R Inhibitor Does Not Alter the Neuroprotective Effects of Adoptive Regulatory T Cells in 3xTg Alzheimer’s Disease Mice. Curr. Issues Mol. Biol. 2024, 46, 2871-2883. https://doi.org/10.3390/cimb46040180
Park S-Y, Cha N, Kim S, Chae S, Lee W-j, Jung H, Bae H. Blocking Microglial Proliferation by CSF-1R Inhibitor Does Not Alter the Neuroprotective Effects of Adoptive Regulatory T Cells in 3xTg Alzheimer’s Disease Mice. Current Issues in Molecular Biology. 2024; 46(4):2871-2883. https://doi.org/10.3390/cimb46040180
Chicago/Turabian StylePark, Seon-Young, Nari Cha, Soyoung Kim, Songah Chae, Won-jun Lee, Hyunjae Jung, and Hyunsu Bae. 2024. "Blocking Microglial Proliferation by CSF-1R Inhibitor Does Not Alter the Neuroprotective Effects of Adoptive Regulatory T Cells in 3xTg Alzheimer’s Disease Mice" Current Issues in Molecular Biology 46, no. 4: 2871-2883. https://doi.org/10.3390/cimb46040180