The Role of Sirtuin-1 Isoforms in Regulating Mitochondrial Function
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and SIRT1 Plasmid Constructs
2.2. Transfections
2.3. Analysis of Mitochondrial Membrane Potential
2.4. Determination of Mitochondrial Respiration Rate, Glycolytic Rate, and ATP Production Rate
2.5. Assessing the Mitochondrial Electron Transport Chain Activity in Intact Cells Using Oroboros High-Resolution Respirometry
2.6. Quantitative Reverse-Transcriptase PCR
2.7. Immunoprecipitation and Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. The SIRT1 Isoforms and Their Impact on Mitochondrial Membrane Potential
3.2. Loss of N-Terminal Region in SIRT1 Isoforms Affected the Regulation of Cellular Metabolism
3.3. Loss of N-Terminal Region in SIRT1 Isoforms Affected the Regulation of Mitochondrial Respiratory Complex I
3.4. Loss of N-Terminal Region in SIRT1 Isoforms Affected the Regulation of Mitochondrial Genes and Histone H4 Acetylation
3.5. The Impact of SIRT1 Isoforms on Cellular Respiration in the SIRT1-Knockout Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patyal, P.; Ameer, F.S.; Verma, A.; Zhang, X.; Azhar, G.; Shrivastava, J.; Sharma, S.; Zhang, R.; Wei, J.Y. The Role of Sirtuin-1 Isoforms in Regulating Mitochondrial Function. Curr. Issues Mol. Biol. 2024, 46, 8835-8851. https://doi.org/10.3390/cimb46080522
Patyal P, Ameer FS, Verma A, Zhang X, Azhar G, Shrivastava J, Sharma S, Zhang R, Wei JY. The Role of Sirtuin-1 Isoforms in Regulating Mitochondrial Function. Current Issues in Molecular Biology. 2024; 46(8):8835-8851. https://doi.org/10.3390/cimb46080522
Chicago/Turabian StylePatyal, Pankaj, Fathima S. Ameer, Ambika Verma, Xiaomin Zhang, Gohar Azhar, Jyotsna Shrivastava, Shakshi Sharma, Rachel Zhang, and Jeanne Y. Wei. 2024. "The Role of Sirtuin-1 Isoforms in Regulating Mitochondrial Function" Current Issues in Molecular Biology 46, no. 8: 8835-8851. https://doi.org/10.3390/cimb46080522