miRNA Sequencing Analysis in Maize Roots Treated with Neutral and Alkaline Salts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Maize Seedling Stress Treatment
2.2. miRNA Sequencing Analysis
2.3. RNA Extraction, Quantification and Quantitative Real Time PCR (qPCR)
2.4. Computational Prediction of miRNA Targets
2.5. Ion Content Determination
3. Results
3.1. Phenotypic Identification of Maize Roots under Neutral Salt and Alkaline Stress
3.2. miRNA Sequencing Results and Data Analysis in Maize Roots under Different Salt Stress Conditions
3.3. Differential miRNA Expression Analysis
3.4. Prediction and Bioinformatics Analysis of Differentially Expressed miRNA Target Genes
3.5. Analysis of Differentially Expressed miRNAs Shared by Different Comparison Groups
3.6. qPCR Validation of Sequencing Results
3.7. The Changes in the Content of Different Ions in Maize Roots under Neutral and Alkaline Salt Stress
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, Z.; Liu, Y.; Wang, Q.; Fei, J.; Liu, X.; Zhang, C.; Yin, Y. miRNA Sequencing Analysis in Maize Roots Treated with Neutral and Alkaline Salts. Curr. Issues Mol. Biol. 2024, 46, 8874-8889. https://doi.org/10.3390/cimb46080524
Chen Z, Liu Y, Wang Q, Fei J, Liu X, Zhang C, Yin Y. miRNA Sequencing Analysis in Maize Roots Treated with Neutral and Alkaline Salts. Current Issues in Molecular Biology. 2024; 46(8):8874-8889. https://doi.org/10.3390/cimb46080524
Chicago/Turabian StyleChen, Ziqi, Yang Liu, Qi Wang, Jianbo Fei, Xiangguo Liu, Chuang Zhang, and Yuejia Yin. 2024. "miRNA Sequencing Analysis in Maize Roots Treated with Neutral and Alkaline Salts" Current Issues in Molecular Biology 46, no. 8: 8874-8889. https://doi.org/10.3390/cimb46080524