Expression of Tenascin-C Is Upregulated in the Early Stages of Radiation Pneumonitis/Fibrosis in a Novel Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice and Pb Shielding Device
2.2. Irradiation and CT Scans
2.3. Histopathological Analysis of Lung Tissue
2.4. Quantification of TNC-Expressing Regions
3. Results
3.1. Sequential CT Image Changes in Focally Irradiated Lung of Mouse with 30 Gy/1fr
3.2. Comparison of CT Image Findings with Pathological Findings
4. Discussion
4.1. Partial Lung Irradiation Model for Mice
4.2. Sequential Changes in CT Images after Irradiation
4.3. Comparison of CT Images and Histological Changes
4.4. TNC Is Upregulated at the Early Stage of Radiation Pneumonitis
4.5. Limitations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Post Irradiation | 12 w | 16 w | 20 w | 24 w | 28 w | 36 w | 44 w | ||
---|---|---|---|---|---|---|---|---|---|
Computed Tomography | − | − | Radiation pneumonia | Radiation fibrsis | |||||
Histology | Inflammation | − | + | + + + | + + | ± | − | − | |
Fibrosis | − | − | + | + | + + | + + + | + + + | ||
Tenascin-C | − | + | + + + | + + | ± | − | − |
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Omori, K.; Takada, A.; Toyomasu, Y.; Tawara, I.; Shintoku, C.; Imanaka-Yoshida, K.; Sakuma, H.; Nomoto, Y. Expression of Tenascin-C Is Upregulated in the Early Stages of Radiation Pneumonitis/Fibrosis in a Novel Mouse Model. Curr. Issues Mol. Biol. 2024, 46, 9674-9685. https://doi.org/10.3390/cimb46090575
Omori K, Takada A, Toyomasu Y, Tawara I, Shintoku C, Imanaka-Yoshida K, Sakuma H, Nomoto Y. Expression of Tenascin-C Is Upregulated in the Early Stages of Radiation Pneumonitis/Fibrosis in a Novel Mouse Model. Current Issues in Molecular Biology. 2024; 46(9):9674-9685. https://doi.org/10.3390/cimb46090575
Chicago/Turabian StyleOmori, Kazuki, Akinori Takada, Yutaka Toyomasu, Isao Tawara, Chihiro Shintoku, Kyoko Imanaka-Yoshida, Hajime Sakuma, and Yoshihito Nomoto. 2024. "Expression of Tenascin-C Is Upregulated in the Early Stages of Radiation Pneumonitis/Fibrosis in a Novel Mouse Model" Current Issues in Molecular Biology 46, no. 9: 9674-9685. https://doi.org/10.3390/cimb46090575