Stimulation of Angiotensin II Receptor Subtype 2 Reduces Preeclampsia-like Symptoms in a Mouse Model of Preeclampsia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. C21 Delivery
2.3. Physiological Measurements
2.4. Flow Cytometric Analyses
2.5. B Cell Subset Analysis
2.6. ELISA
2.7. Statistical Analysis
3. Results
3.1. BP and Proteinuria
3.2. Pregnancy Outcomes
3.3. Intracellular Cytokine Profiles
3.4. Flow Cytometry for B Cell Subsets Analysis
3.5. Cytokine Plasma Concentrations
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
List of Abbreviations
AngII | angiotensin II |
AT1R | angiotensin II receptor subtype 1 |
AT2R | angiotensin II receptor subtype 2 |
C21 | compound 21 |
ICR | imprinting control region |
PE | preeclampsia |
sFlt-1 | soluble fms-like tyrosine kinase 1 |
sEng | soluble endoglin |
CD40 | cluster of differentiation 40 |
CD40L | CD40 ligand |
TNF | tumor necrosis factor |
Th | T-helper cell |
RAS | renin–angiotensin system |
BP | blood pressure |
ARBs | angiotensin receptor blockers |
ACEIs | angiotensin-converting enzyme inhibitors |
B2R | bradykinin B2 receptor |
IPF | idiopathic pulmonary fibrosis |
FVC | forced vital capacity |
LacZ | β-galactosidase |
ELISA | enzyme-linked immunosorbent assay |
RT | room temperature |
IFN-γ | interferon-γ |
IL-4 | interleukin-4 |
VEGFR1 | vascular endothelial growth factor receptor 1 |
ANOVAs | one-way analysis of variance |
PlGF | placental growth factor |
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Matsubara, K.; Matsubara, Y.; Uchikura, Y.; Sugiyama, T. Stimulation of Angiotensin II Receptor Subtype 2 Reduces Preeclampsia-like Symptoms in a Mouse Model of Preeclampsia. Curr. Issues Mol. Biol. 2024, 46, 9760-9771. https://doi.org/10.3390/cimb46090579
Matsubara K, Matsubara Y, Uchikura Y, Sugiyama T. Stimulation of Angiotensin II Receptor Subtype 2 Reduces Preeclampsia-like Symptoms in a Mouse Model of Preeclampsia. Current Issues in Molecular Biology. 2024; 46(9):9760-9771. https://doi.org/10.3390/cimb46090579
Chicago/Turabian StyleMatsubara, Keiichi, Yuko Matsubara, Yuka Uchikura, and Takashi Sugiyama. 2024. "Stimulation of Angiotensin II Receptor Subtype 2 Reduces Preeclampsia-like Symptoms in a Mouse Model of Preeclampsia" Current Issues in Molecular Biology 46, no. 9: 9760-9771. https://doi.org/10.3390/cimb46090579