The Diterpene Isopimaric Acid Modulates the Phytohormone Pathway to Promote Oryza sativa L. Rice Seedling Growth
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Culture Conditions
2.2. Extraction of Isopimaric Acid
2.3. Preparation of Reagent Solutions
2.4. Growth of Rice with Different Concentrations of Isopimaric and Gibberellic Acids
2.5. Analysis of Seedling Growth
2.6. Extraction and Quantitative Analysis of Phytohormones
2.7. Phytohormone Standard Curve
2.8. Statistical Analyses
3. Results
3.1. Isopimaric Acid Promotes Rice Seedling Growth
3.2. Isopimaric Acid Activates the Endogenous Phytohormone Pathway Affecting Rice Growth
3.2.1. Quantification of IAA-Related Phytohormones in Seedlings Showing Growth Promotion
3.2.2. Quantification of Cytokinin Concentrations in Rice Seedlings Following Isopimaric Acid Treatment
3.2.3. Quantitative Analysis of Gibberellin-Related (GA-Related) Phytohormones in Rice Seedlings Following Treatment with Isopimaric Acid
3.2.4. Quantification of Defense-Related Phytohormone Concentrations in Rice Seedlings Following Treatment with Isopimaric Acid
3.3. Correlation Analysis of Phytohormone Concentrations with Growth Characteristics in Rice Seedlings Following Treatment with Isopimaric Acid
3.3.1. Correlation Analysis of Growth-Related Phytohormone Concentrations with Growth Characteristics in Rice Seedlings
3.3.2. Correlation of Concentrations of Defense-Related Phytohormones with Growth Characteristics in Rice Seedlings
4. Discussion
4.1. The Diterpene Isopimaric Acid Could Be Developed as a Promising Plant-Derived Growth Regulator
4.2. Isopimaric Acid May Act through Phytohormone Pathways to Promote Rice Growth
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Huang, J.; Hua, J.; Peng, L.; Bai, L.; Luo, S. The Diterpene Isopimaric Acid Modulates the Phytohormone Pathway to Promote Oryza sativa L. Rice Seedling Growth. Curr. Issues Mol. Biol. 2024, 46, 9772-9784. https://doi.org/10.3390/cimb46090580
Huang J, Hua J, Peng L, Bai L, Luo S. The Diterpene Isopimaric Acid Modulates the Phytohormone Pathway to Promote Oryza sativa L. Rice Seedling Growth. Current Issues in Molecular Biology. 2024; 46(9):9772-9784. https://doi.org/10.3390/cimb46090580
Chicago/Turabian StyleHuang, Jiaqi, Juan Hua, Luying Peng, Liping Bai, and Shihong Luo. 2024. "The Diterpene Isopimaric Acid Modulates the Phytohormone Pathway to Promote Oryza sativa L. Rice Seedling Growth" Current Issues in Molecular Biology 46, no. 9: 9772-9784. https://doi.org/10.3390/cimb46090580