Effect of (1→3),(1→6)-β-Glucan on In Vitro Production of Cytokines by Leukocytes of Patients With Periodontitis
Abstract
Material and Methods. In total, 20 patients suffering from untreated severe chronic generalized periodontitis were enrolled in this study. Periodontitis was confirmed by clinical and radiologic examination. Besides, 20 periodontally healthy patients served as a control group. Peripheral venous blood was sampled from the patients, and isolated leukocytes were treated with (1→3),(1→6)-β-glucan from yeast at different concentrations. The levels of IL-10 and TNF-α secreted by the leukocytes unstimulated and stimulated with unopsonized E. coli in vitro were determined by the enzyme amplified sensitivity immunoassay method.
Results. Our data showed that (1→3),(1→6)-β-glucan induced a significant decrease (P<0.05) in the TNF-α level and a significant increase (P<0.001) in the IL-10 level in the media of unstimulated and stimulated leukocytes of the patients with periodontitis in comparison with those of the healthy subjects.
Conclusions. The present in vitro study showed that (1→3),(1→6)-β-glucan modulated the response of leukocytes of the patients with periodontitis differently in comparison with those of the healthy subjects. It increased the release of IL-10, which is protective of the tooth-supporting tissues in patients with periodontal disease, but decreased the release of TNF-α, which is mainly responsible for the destruction of the tooth-supporting tissues during periodontal disease.
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Akramas, L.; Akramienė, D.; Sakalauskienė, J.; Kubilius, R.; Gleiznys, A. Effect of (1→3),(1→6)-β-Glucan on In Vitro Production of Cytokines by Leukocytes of Patients With Periodontitis. Medicina 2012, 48, 27. https://doi.org/10.3390/medicina48040027
Akramas L, Akramienė D, Sakalauskienė J, Kubilius R, Gleiznys A. Effect of (1→3),(1→6)-β-Glucan on In Vitro Production of Cytokines by Leukocytes of Patients With Periodontitis. Medicina. 2012; 48(4):27. https://doi.org/10.3390/medicina48040027
Chicago/Turabian StyleAkramas, Laimis, Dalia Akramienė, Jurgina Sakalauskienė, Ričardas Kubilius, and Alvydas Gleiznys. 2012. "Effect of (1→3),(1→6)-β-Glucan on In Vitro Production of Cytokines by Leukocytes of Patients With Periodontitis" Medicina 48, no. 4: 27. https://doi.org/10.3390/medicina48040027